Characteristics of bacteria showing high denitrification activity in saline wastewater

S. Yoshie, T. Ogawa, H. Makino, H. Hirosawa, Satoshi Tsuneda, A. Hirata

    Research output: Contribution to journalArticle

    32 Citations (Scopus)

    Abstract

    Aims: Denitrification efficiency at 10% salinity was compared with that at 2% salinity. The characteristics of bacterial strains isolated from the denitrification system, where an improvement of denitrification efficiency was observed at a high salinity were investigated. Methods and Results: Two continuous feeding denitrification systems for saline solutions of 2% and 10% salinity, were operated. Denitrification efficiency at 10% salinity was higher than that at 2% salinity. The bacterial strains were isolated using the trypticase soy agar (TSA) medium at 30°C. The phylogenetic analysis of 16S rRNA gene sequences of isolates indicated that halophilic species were predominant at 10% salinity. Conclusions: The improvement of denitrification efficiency at a high salinity was demonstrated. The strains isolated from the denitrifying system with 10% salinity were halophilic bacteria, Halomonas sp. and Marinobacter sp., suggesting that these bacteria show a high denitrifying activity at 10% salinity. Significance and Impact of the Study: The long-term acclimated sludge used in this study resulted in high denitrification performance at a high salinity, indicating that the design of a high-performance denitrification system for saline wastewater will be possible.

    Original languageEnglish
    Pages (from-to)277-283
    Number of pages7
    JournalLetters in Applied Microbiology
    Volume42
    Issue number3
    DOIs
    Publication statusPublished - 2006 Mar

    Fingerprint

    Denitrification
    Salinity
    Waste Water
    denitrification
    Bacteria
    Wastewater
    Thermodynamic properties
    salinity
    wastewater
    bacterium
    Marinobacter
    Halomonas
    Sodium Chloride
    Agar
    Genes
    Sewage
    rRNA Genes
    agar
    sludge

    Keywords

    • Halomonas
    • Halophilic denitrifying bacteria
    • Improvement of denitrification efficiency
    • Isolation
    • Marinobacter
    • Wastewater treatment system

    ASJC Scopus subject areas

    • Applied Microbiology and Biotechnology
    • Water Science and Technology
    • Fluid Flow and Transfer Processes
    • Microbiology
    • Ecology

    Cite this

    Characteristics of bacteria showing high denitrification activity in saline wastewater. / Yoshie, S.; Ogawa, T.; Makino, H.; Hirosawa, H.; Tsuneda, Satoshi; Hirata, A.

    In: Letters in Applied Microbiology, Vol. 42, No. 3, 03.2006, p. 277-283.

    Research output: Contribution to journalArticle

    Yoshie, S. ; Ogawa, T. ; Makino, H. ; Hirosawa, H. ; Tsuneda, Satoshi ; Hirata, A. / Characteristics of bacteria showing high denitrification activity in saline wastewater. In: Letters in Applied Microbiology. 2006 ; Vol. 42, No. 3. pp. 277-283.
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