Characteristics of Hb-vesicles and encapsulation procedure

Shinji Takeoka, H. Sakai, K. Terase, Hiroyuki Nishide, E. Tsuchida

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

The performance of Hb-vesicles depends on the weight ratio of Hb to lipid ([Hb]/[Lipid]). This value is improved by lowering the number of bilayer membrane of the vesicle and raising the concentration of Hb in the interior of the vesicle. Maximum [Hb]/[Lipid] ratio was obtained at ca. pH 7, that would relate to the isoelectric point (pI) of Hb at 25°C. On the other hand, the [Hb]/[Lipid] ratio decreased with ionic strength and increased with lowering temperature. The Hb-vesicles encapsulating 40 g/dl Hb with only one bilayer membrane were isolated by using the difference in the density of the vesicles.

Original languageEnglish
Pages (from-to)861-866
Number of pages6
JournalArtificial Cells, Blood Substitutes, and Immobilization Biotechnology
Volume22
Issue number3
Publication statusPublished - 1994

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ASJC Scopus subject areas

  • Biotechnology
  • Biomedical Engineering
  • Biomaterials
  • Hematology

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