Characterization and cloning of TMV resistance gene n homologues from nicotiana tabacum

Jun Shan Gao, Yan Meng, Nobumitsu Sasaki, Hiromi Kanegae, Naomi Hayashi, Hiroshi Nyunoya

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Tobacco cultivars Nicotiana tabacum cv. Samsun NN plants carrying the N gene contain a multitude of N-related genes. We cloned a few N homologues and isolated two full-length cDNAs of NL-C26 and NL-B69 genes from N. tabacum cv. Samsun NN. Nucleotide sequence analysis showed that the coding regions of NL-C26 (3,498 bp) and NL-B69 (3,510 bp) had 86 and 83% nucleotide identities with the N gene, respectively. Amino acid sequence analysis revealed that NL-C26 and NL-B69 had the Toll-interleukin-1 receptor/nucleotide-binding site/leucine-rich repeat (TIR/NBS/LRR) structure with 78 and 73% identities to N, respectively. These result indicated that NL-C26 was more similar to N than NL-B69. Tobacco mosaic virus (TMV) infection experiments suggest that NL-C26 and NL-B69 could interact with distinct avirulence (Avr) proteins of yet unidentified pathogens and function as potential HR-inducing resistance proteins similar to N.

Original languageEnglish
Pages (from-to)7998-8006
Number of pages9
JournalAfrican Journal of Biotechnology
Volume9
Issue number47
DOIs
Publication statusPublished - 2010 Nov 22

Keywords

  • Hypersensitive response
  • N gene
  • N homologue
  • Nicotiana tabacum
  • Tobacco mosaic virus (TMV)

ASJC Scopus subject areas

  • Biotechnology
  • Applied Microbiology and Biotechnology
  • Molecular Biology
  • Genetics
  • Agronomy and Crop Science

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