Characterization and cloning of TMV resistance gene n homologues from nicotiana tabacum

Jun Shan Gao, Yan Meng, Nobumitsu Sasaki, Hiromi Kanegae, Naomi Hayashi, Hiroshi Nyunoya

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Tobacco cultivars Nicotiana tabacum cv. Samsun NN plants carrying the N gene contain a multitude of N-related genes. We cloned a few N homologues and isolated two full-length cDNAs of NL-C26 and NL-B69 genes from N. tabacum cv. Samsun NN. Nucleotide sequence analysis showed that the coding regions of NL-C26 (3,498 bp) and NL-B69 (3,510 bp) had 86 and 83% nucleotide identities with the N gene, respectively. Amino acid sequence analysis revealed that NL-C26 and NL-B69 had the Toll-interleukin-1 receptor/nucleotide-binding site/leucine-rich repeat (TIR/NBS/LRR) structure with 78 and 73% identities to N, respectively. These result indicated that NL-C26 was more similar to N than NL-B69. Tobacco mosaic virus (TMV) infection experiments suggest that NL-C26 and NL-B69 could interact with distinct avirulence (Avr) proteins of yet unidentified pathogens and function as potential HR-inducing resistance proteins similar to N.

Original languageEnglish
Pages (from-to)7998-8006
Number of pages9
JournalAfrican Journal of Biotechnology
Volume9
Issue number47
Publication statusPublished - 2010 Nov 22
Externally publishedYes

Fingerprint

Tobacco Mosaic Virus
Tobacco mosaic virus
Nicotiana tabacum
Tobacco
Organism Cloning
molecular cloning
Genes
genes
Nucleotides
sequence analysis
nucleotides
Interleukin-1 Receptors
Protein Sequence Analysis
interleukin-1
Virus Diseases
Leucine
leucine
Sequence Analysis
binding sites
Proteins

Keywords

  • Hypersensitive response
  • N gene
  • N homologue
  • Nicotiana tabacum
  • Tobacco mosaic virus (TMV)

ASJC Scopus subject areas

  • Biotechnology
  • Applied Microbiology and Biotechnology
  • Genetics
  • Molecular Biology
  • Agronomy and Crop Science

Cite this

Characterization and cloning of TMV resistance gene n homologues from nicotiana tabacum. / Gao, Jun Shan; Meng, Yan; Sasaki, Nobumitsu; Kanegae, Hiromi; Hayashi, Naomi; Nyunoya, Hiroshi.

In: African Journal of Biotechnology, Vol. 9, No. 47, 22.11.2010, p. 7998-8006.

Research output: Contribution to journalArticle

Gao, JS, Meng, Y, Sasaki, N, Kanegae, H, Hayashi, N & Nyunoya, H 2010, 'Characterization and cloning of TMV resistance gene n homologues from nicotiana tabacum', African Journal of Biotechnology, vol. 9, no. 47, pp. 7998-8006.
Gao, Jun Shan ; Meng, Yan ; Sasaki, Nobumitsu ; Kanegae, Hiromi ; Hayashi, Naomi ; Nyunoya, Hiroshi. / Characterization and cloning of TMV resistance gene n homologues from nicotiana tabacum. In: African Journal of Biotechnology. 2010 ; Vol. 9, No. 47. pp. 7998-8006.
@article{b32c5639ad724eaf8ff4ef12fdd06809,
title = "Characterization and cloning of TMV resistance gene n homologues from nicotiana tabacum",
abstract = "Tobacco cultivars Nicotiana tabacum cv. Samsun NN plants carrying the N gene contain a multitude of N-related genes. We cloned a few N homologues and isolated two full-length cDNAs of NL-C26 and NL-B69 genes from N. tabacum cv. Samsun NN. Nucleotide sequence analysis showed that the coding regions of NL-C26 (3,498 bp) and NL-B69 (3,510 bp) had 86 and 83{\%} nucleotide identities with the N gene, respectively. Amino acid sequence analysis revealed that NL-C26 and NL-B69 had the Toll-interleukin-1 receptor/nucleotide-binding site/leucine-rich repeat (TIR/NBS/LRR) structure with 78 and 73{\%} identities to N, respectively. These result indicated that NL-C26 was more similar to N than NL-B69. Tobacco mosaic virus (TMV) infection experiments suggest that NL-C26 and NL-B69 could interact with distinct avirulence (Avr) proteins of yet unidentified pathogens and function as potential HR-inducing resistance proteins similar to N.",
keywords = "Hypersensitive response, N gene, N homologue, Nicotiana tabacum, Tobacco mosaic virus (TMV)",
author = "Gao, {Jun Shan} and Yan Meng and Nobumitsu Sasaki and Hiromi Kanegae and Naomi Hayashi and Hiroshi Nyunoya",
year = "2010",
month = "11",
day = "22",
language = "English",
volume = "9",
pages = "7998--8006",
journal = "African Journal of Biotechnology",
issn = "1684-5315",
publisher = "Academic Journals",
number = "47",

}

TY - JOUR

T1 - Characterization and cloning of TMV resistance gene n homologues from nicotiana tabacum

AU - Gao, Jun Shan

AU - Meng, Yan

AU - Sasaki, Nobumitsu

AU - Kanegae, Hiromi

AU - Hayashi, Naomi

AU - Nyunoya, Hiroshi

PY - 2010/11/22

Y1 - 2010/11/22

N2 - Tobacco cultivars Nicotiana tabacum cv. Samsun NN plants carrying the N gene contain a multitude of N-related genes. We cloned a few N homologues and isolated two full-length cDNAs of NL-C26 and NL-B69 genes from N. tabacum cv. Samsun NN. Nucleotide sequence analysis showed that the coding regions of NL-C26 (3,498 bp) and NL-B69 (3,510 bp) had 86 and 83% nucleotide identities with the N gene, respectively. Amino acid sequence analysis revealed that NL-C26 and NL-B69 had the Toll-interleukin-1 receptor/nucleotide-binding site/leucine-rich repeat (TIR/NBS/LRR) structure with 78 and 73% identities to N, respectively. These result indicated that NL-C26 was more similar to N than NL-B69. Tobacco mosaic virus (TMV) infection experiments suggest that NL-C26 and NL-B69 could interact with distinct avirulence (Avr) proteins of yet unidentified pathogens and function as potential HR-inducing resistance proteins similar to N.

AB - Tobacco cultivars Nicotiana tabacum cv. Samsun NN plants carrying the N gene contain a multitude of N-related genes. We cloned a few N homologues and isolated two full-length cDNAs of NL-C26 and NL-B69 genes from N. tabacum cv. Samsun NN. Nucleotide sequence analysis showed that the coding regions of NL-C26 (3,498 bp) and NL-B69 (3,510 bp) had 86 and 83% nucleotide identities with the N gene, respectively. Amino acid sequence analysis revealed that NL-C26 and NL-B69 had the Toll-interleukin-1 receptor/nucleotide-binding site/leucine-rich repeat (TIR/NBS/LRR) structure with 78 and 73% identities to N, respectively. These result indicated that NL-C26 was more similar to N than NL-B69. Tobacco mosaic virus (TMV) infection experiments suggest that NL-C26 and NL-B69 could interact with distinct avirulence (Avr) proteins of yet unidentified pathogens and function as potential HR-inducing resistance proteins similar to N.

KW - Hypersensitive response

KW - N gene

KW - N homologue

KW - Nicotiana tabacum

KW - Tobacco mosaic virus (TMV)

UR - http://www.scopus.com/inward/record.url?scp=78649639245&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=78649639245&partnerID=8YFLogxK

M3 - Article

VL - 9

SP - 7998

EP - 8006

JO - African Journal of Biotechnology

JF - African Journal of Biotechnology

SN - 1684-5315

IS - 47

ER -