Characterization and tRNA recognition of mammalian mitochondrial seryl- tRNA synthetase

Takashi Yokogawa, Nobukazu Shimada, Nono Takeuchi, Lisa Benkowski, Tsutomu Suzuki, Akira Omori, Takuya Ueda, Kazuya Nishikawa, Linda L. Spremulli, Kimitsuna Watanabe

Research output: Contribution to journalArticle

37 Citations (Scopus)

Abstract

Animal mitochondrial protein synthesis systems contain two serine tRNAs (tRNAs(Ser)) corresponding to the codons AGY and UCN, each possessing an unusual secondary structure; the former lacks the entire D arm, and the latter has a slightly different cloverleaf structure. To elucidate whether these two tRNAs(Ser) can be recognized by the single animal mitochondrial seryl-tRNA synthetase (mt SerRS), we purified mt SerRS from bovine liver 2400-fold and showed that it can aminoacylate both of them. Specific interaction between mt SerRS and either of the tRNAs(Ser) was also observed in a gel retardation assay. cDNA cloning of bovine mt SerRS revealed that the deduced amino acid sequence of the enzyme contains 518 amino acid residues. The cDNAs of human and mouse mt SerRS were obtained by reverse transcription- polymerase chain reaction and expressed sequence tag data base searches. Elaborate inspection of primary sequences of mammalian mt SerRSs revealed diversity in the N-terminal domain responsible for tRNA recognition, indicating that the recognition mechanism of mammalian mt SerRS differs considerably from that of its prokaryotic counterpart. In addition, the human mt SerRS gene was found to be located on chromosome 19q13.1, to which the autosomal deafness locus DFNA4 is mapped.

Original languageEnglish
Pages (from-to)19913-19920
Number of pages8
JournalJournal of Biological Chemistry
Volume275
Issue number26
DOIs
Publication statusPublished - 2000 Jun 30
Externally publishedYes

Fingerprint

Serine-tRNA Ligase
Transfer RNA
RNA, Transfer, Ser
Animals
Complementary DNA
Amino Acids
Cloning
Polymerase chain reaction
Mitochondrial Proteins
Expressed Sequence Tags
Electrophoretic Mobility Shift Assay
Deafness
Transcription
Chromosomes
Codon
Liver
Serine
Reverse Transcription
Organism Cloning
Amino Acid Sequence

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Yokogawa, T., Shimada, N., Takeuchi, N., Benkowski, L., Suzuki, T., Omori, A., ... Watanabe, K. (2000). Characterization and tRNA recognition of mammalian mitochondrial seryl- tRNA synthetase. Journal of Biological Chemistry, 275(26), 19913-19920. https://doi.org/10.1074/jbc.M908473199

Characterization and tRNA recognition of mammalian mitochondrial seryl- tRNA synthetase. / Yokogawa, Takashi; Shimada, Nobukazu; Takeuchi, Nono; Benkowski, Lisa; Suzuki, Tsutomu; Omori, Akira; Ueda, Takuya; Nishikawa, Kazuya; Spremulli, Linda L.; Watanabe, Kimitsuna.

In: Journal of Biological Chemistry, Vol. 275, No. 26, 30.06.2000, p. 19913-19920.

Research output: Contribution to journalArticle

Yokogawa, T, Shimada, N, Takeuchi, N, Benkowski, L, Suzuki, T, Omori, A, Ueda, T, Nishikawa, K, Spremulli, LL & Watanabe, K 2000, 'Characterization and tRNA recognition of mammalian mitochondrial seryl- tRNA synthetase', Journal of Biological Chemistry, vol. 275, no. 26, pp. 19913-19920. https://doi.org/10.1074/jbc.M908473199
Yokogawa T, Shimada N, Takeuchi N, Benkowski L, Suzuki T, Omori A et al. Characterization and tRNA recognition of mammalian mitochondrial seryl- tRNA synthetase. Journal of Biological Chemistry. 2000 Jun 30;275(26):19913-19920. https://doi.org/10.1074/jbc.M908473199
Yokogawa, Takashi ; Shimada, Nobukazu ; Takeuchi, Nono ; Benkowski, Lisa ; Suzuki, Tsutomu ; Omori, Akira ; Ueda, Takuya ; Nishikawa, Kazuya ; Spremulli, Linda L. ; Watanabe, Kimitsuna. / Characterization and tRNA recognition of mammalian mitochondrial seryl- tRNA synthetase. In: Journal of Biological Chemistry. 2000 ; Vol. 275, No. 26. pp. 19913-19920.
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