Characterization of a flavin reductase from a thermophilic dibenzothiophene-desulfurizing bacterium, Bacillus subtilis WU-S2B

Shusuke Takahashi, Toshiki Furuya, Yoshitaka Ishii, Kuniki Kino, Kotaro Kirimura

    Research output: Contribution to journalArticle

    9 Citations (Scopus)

    Abstract

    Bacillus subtilis WU-S2B is a thermophilic dibenzothiophene (DBT)-desulfurizing bacterium and produces a flavin reductase (Frb) that couples with DBT and DBT sulfone monooxygenases. The recombinant Frb was purified from Escherichia coli cells expressing the frb gene and was characterized. The purified Frb exhibited high stability over wide temperature and pH ranges of 20-55 °C and 2-12, respectively. Frb contained FMN and exhibited both flavin reductase and nitroreductase activities.

    Original languageEnglish
    Pages (from-to)38-41
    Number of pages4
    JournalJournal of Bioscience and Bioengineering
    Volume107
    Issue number1
    DOIs
    Publication statusPublished - 2009 Jan

    Fingerprint

    Bacilli
    Desulfurization
    Bacillus subtilis
    Bacteria
    Oxidoreductases
    Nitroreductases
    Flavin Mononucleotide
    Escherichia coli
    Genes
    Cells
    Temperature
    dibenzothiophene
    4,6-dinitro-o-cresol
    dibenzothiophene sulfone monooxygenase
    flavin nitroreductase

    Keywords

    • Bacillus subtilis
    • Desulfurization
    • Flavin reductase
    • Flavin-dependent monooxygenase
    • Nitroreductase

    ASJC Scopus subject areas

    • Biotechnology
    • Applied Microbiology and Biotechnology
    • Bioengineering

    Cite this

    Characterization of a flavin reductase from a thermophilic dibenzothiophene-desulfurizing bacterium, Bacillus subtilis WU-S2B. / Takahashi, Shusuke; Furuya, Toshiki; Ishii, Yoshitaka; Kino, Kuniki; Kirimura, Kotaro.

    In: Journal of Bioscience and Bioengineering, Vol. 107, No. 1, 01.2009, p. 38-41.

    Research output: Contribution to journalArticle

    @article{c443a63b3eba4464af3bdc7b7fff43e8,
    title = "Characterization of a flavin reductase from a thermophilic dibenzothiophene-desulfurizing bacterium, Bacillus subtilis WU-S2B",
    abstract = "Bacillus subtilis WU-S2B is a thermophilic dibenzothiophene (DBT)-desulfurizing bacterium and produces a flavin reductase (Frb) that couples with DBT and DBT sulfone monooxygenases. The recombinant Frb was purified from Escherichia coli cells expressing the frb gene and was characterized. The purified Frb exhibited high stability over wide temperature and pH ranges of 20-55 °C and 2-12, respectively. Frb contained FMN and exhibited both flavin reductase and nitroreductase activities.",
    keywords = "Bacillus subtilis, Desulfurization, Flavin reductase, Flavin-dependent monooxygenase, Nitroreductase",
    author = "Shusuke Takahashi and Toshiki Furuya and Yoshitaka Ishii and Kuniki Kino and Kotaro Kirimura",
    year = "2009",
    month = "1",
    doi = "10.1016/j.jbiosc.2008.09.008",
    language = "English",
    volume = "107",
    pages = "38--41",
    journal = "Journal of Bioscience and Bioengineering",
    issn = "1389-1723",
    publisher = "Elsevier",
    number = "1",

    }

    TY - JOUR

    T1 - Characterization of a flavin reductase from a thermophilic dibenzothiophene-desulfurizing bacterium, Bacillus subtilis WU-S2B

    AU - Takahashi, Shusuke

    AU - Furuya, Toshiki

    AU - Ishii, Yoshitaka

    AU - Kino, Kuniki

    AU - Kirimura, Kotaro

    PY - 2009/1

    Y1 - 2009/1

    N2 - Bacillus subtilis WU-S2B is a thermophilic dibenzothiophene (DBT)-desulfurizing bacterium and produces a flavin reductase (Frb) that couples with DBT and DBT sulfone monooxygenases. The recombinant Frb was purified from Escherichia coli cells expressing the frb gene and was characterized. The purified Frb exhibited high stability over wide temperature and pH ranges of 20-55 °C and 2-12, respectively. Frb contained FMN and exhibited both flavin reductase and nitroreductase activities.

    AB - Bacillus subtilis WU-S2B is a thermophilic dibenzothiophene (DBT)-desulfurizing bacterium and produces a flavin reductase (Frb) that couples with DBT and DBT sulfone monooxygenases. The recombinant Frb was purified from Escherichia coli cells expressing the frb gene and was characterized. The purified Frb exhibited high stability over wide temperature and pH ranges of 20-55 °C and 2-12, respectively. Frb contained FMN and exhibited both flavin reductase and nitroreductase activities.

    KW - Bacillus subtilis

    KW - Desulfurization

    KW - Flavin reductase

    KW - Flavin-dependent monooxygenase

    KW - Nitroreductase

    UR - http://www.scopus.com/inward/record.url?scp=58149310658&partnerID=8YFLogxK

    UR - http://www.scopus.com/inward/citedby.url?scp=58149310658&partnerID=8YFLogxK

    U2 - 10.1016/j.jbiosc.2008.09.008

    DO - 10.1016/j.jbiosc.2008.09.008

    M3 - Article

    VL - 107

    SP - 38

    EP - 41

    JO - Journal of Bioscience and Bioengineering

    JF - Journal of Bioscience and Bioengineering

    SN - 1389-1723

    IS - 1

    ER -