Characterization of a flavin reductase from a thermophilic dibenzothiophene-desulfurizing bacterium, Bacillus subtilis WU-S2B

Shusuke Takahashi, Toshiki Furuya, Yoshitaka Ishii, Kuniki Kino, Kotaro Kirimura

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    10 Citations (Scopus)

    Abstract

    Bacillus subtilis WU-S2B is a thermophilic dibenzothiophene (DBT)-desulfurizing bacterium and produces a flavin reductase (Frb) that couples with DBT and DBT sulfone monooxygenases. The recombinant Frb was purified from Escherichia coli cells expressing the frb gene and was characterized. The purified Frb exhibited high stability over wide temperature and pH ranges of 20-55 °C and 2-12, respectively. Frb contained FMN and exhibited both flavin reductase and nitroreductase activities.

    Original languageEnglish
    Pages (from-to)38-41
    Number of pages4
    JournalJournal of Bioscience and Bioengineering
    Volume107
    Issue number1
    DOIs
    Publication statusPublished - 2009 Jan

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    Keywords

    • Bacillus subtilis
    • Desulfurization
    • Flavin reductase
    • Flavin-dependent monooxygenase
    • Nitroreductase

    ASJC Scopus subject areas

    • Biotechnology
    • Applied Microbiology and Biotechnology
    • Bioengineering

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