Characterization of a flavin reductase from a thermophilic dibenzothiophene-desulfurizing bacterium, Bacillus subtilis WU-S2B

Shusuke Takahashi, Toshiki Furuya, Yoshitaka Ishii, Kuniki Kino, Kohtaro Kirimura

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Bacillus subtilis WU-S2B is a thermophilic dibenzothiophene (DBT)-desulfurizing bacterium and produces a flavin reductase (Frb) that couples with DBT and DBT sulfone monooxygenases. The recombinant Frb was purified from Escherichia coli cells expressing the frb gene and was characterized. The purified Frb exhibited high stability over wide temperature and pH ranges of 20-55 °C and 2-12, respectively. Frb contained FMN and exhibited both flavin reductase and nitroreductase activities.

Original languageEnglish
Pages (from-to)38-41
Number of pages4
JournalJournal of Bioscience and Bioengineering
Volume107
Issue number1
DOIs
Publication statusPublished - 2009 Jan 1

Keywords

  • Bacillus subtilis
  • Desulfurization
  • Flavin reductase
  • Flavin-dependent monooxygenase
  • Nitroreductase

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology

Fingerprint Dive into the research topics of 'Characterization of a flavin reductase from a thermophilic dibenzothiophene-desulfurizing bacterium, Bacillus subtilis WU-S2B'. Together they form a unique fingerprint.

  • Cite this