Characterization of a flavin reductase from a thermophilic dibenzothiophene-desulfurizing bacterium, Bacillus subtilis WU-S2B

Shusuke Takahashi; Toshiki Furuya; Yoshitaka Ishii; Kuniki Kino; Kohtaro Kirimura

doi:10.1016/j.jbiosc.2008.09.008

Characterization of a flavin reductase from a thermophilic dibenzothiophene-desulfurizing bacterium, Bacillus subtilis WU-S2B

Shusuke Takahashi, Toshiki Furuya, Yoshitaka Ishii, Kuniki Kino, Kohtaro Kirimura^*

^*Corresponding author for this work

School of Advanced Science and Engineering

Research output: Contribution to journal › Article › peer-review

10 Citations (Scopus)

Abstract

Bacillus subtilis WU-S2B is a thermophilic dibenzothiophene (DBT)-desulfurizing bacterium and produces a flavin reductase (Frb) that couples with DBT and DBT sulfone monooxygenases. The recombinant Frb was purified from Escherichia coli cells expressing the frb gene and was characterized. The purified Frb exhibited high stability over wide temperature and pH ranges of 20-55 °C and 2-12, respectively. Frb contained FMN and exhibited both flavin reductase and nitroreductase activities.

Original language	English
Pages (from-to)	38-41
Number of pages	4
Journal	Journal of Bioscience and Bioengineering
Volume	107
Issue number	1
DOIs	https://doi.org/10.1016/j.jbiosc.2008.09.008
Publication status	Published - 2009 Jan

Keywords

Bacillus subtilis
Desulfurization
Flavin reductase
Flavin-dependent monooxygenase
Nitroreductase

ASJC Scopus subject areas

Biotechnology
Bioengineering
Applied Microbiology and Biotechnology

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10.1016/j.jbiosc.2008.09.008

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title = "Characterization of a flavin reductase from a thermophilic dibenzothiophene-desulfurizing bacterium, Bacillus subtilis WU-S2B",

abstract = "Bacillus subtilis WU-S2B is a thermophilic dibenzothiophene (DBT)-desulfurizing bacterium and produces a flavin reductase (Frb) that couples with DBT and DBT sulfone monooxygenases. The recombinant Frb was purified from Escherichia coli cells expressing the frb gene and was characterized. The purified Frb exhibited high stability over wide temperature and pH ranges of 20-55 °C and 2-12, respectively. Frb contained FMN and exhibited both flavin reductase and nitroreductase activities.",

keywords = "Bacillus subtilis, Desulfurization, Flavin reductase, Flavin-dependent monooxygenase, Nitroreductase",

author = "Shusuke Takahashi and Toshiki Furuya and Yoshitaka Ishii and Kuniki Kino and Kohtaro Kirimura",

note = "Funding Information: This work was partially supported by the Global COE program “Center for Practical Chemical Wisdom” by the Ministry of Education, Culture, Sports, Science and Technology (MEXT), Japan.",

year = "2009",

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doi = "10.1016/j.jbiosc.2008.09.008",

language = "English",

volume = "107",

pages = "38--41",

journal = "Journal of Bioscience and Bioengineering",

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T1 - Characterization of a flavin reductase from a thermophilic dibenzothiophene-desulfurizing bacterium, Bacillus subtilis WU-S2B

AU - Takahashi, Shusuke

AU - Furuya, Toshiki

AU - Ishii, Yoshitaka

AU - Kino, Kuniki

AU - Kirimura, Kohtaro

N1 - Funding Information: This work was partially supported by the Global COE program “Center for Practical Chemical Wisdom” by the Ministry of Education, Culture, Sports, Science and Technology (MEXT), Japan.

PY - 2009/1

Y1 - 2009/1

N2 - Bacillus subtilis WU-S2B is a thermophilic dibenzothiophene (DBT)-desulfurizing bacterium and produces a flavin reductase (Frb) that couples with DBT and DBT sulfone monooxygenases. The recombinant Frb was purified from Escherichia coli cells expressing the frb gene and was characterized. The purified Frb exhibited high stability over wide temperature and pH ranges of 20-55 °C and 2-12, respectively. Frb contained FMN and exhibited both flavin reductase and nitroreductase activities.

AB - Bacillus subtilis WU-S2B is a thermophilic dibenzothiophene (DBT)-desulfurizing bacterium and produces a flavin reductase (Frb) that couples with DBT and DBT sulfone monooxygenases. The recombinant Frb was purified from Escherichia coli cells expressing the frb gene and was characterized. The purified Frb exhibited high stability over wide temperature and pH ranges of 20-55 °C and 2-12, respectively. Frb contained FMN and exhibited both flavin reductase and nitroreductase activities.

KW - Bacillus subtilis

KW - Desulfurization

KW - Flavin reductase

KW - Flavin-dependent monooxygenase

KW - Nitroreductase

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SN - 1389-1723

VL - 107

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JO - Journal of Bioscience and Bioengineering

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Characterization of a flavin reductase from a thermophilic dibenzothiophene-desulfurizing bacterium, Bacillus subtilis WU-S2B

Abstract

Keywords

ASJC Scopus subject areas

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