TY - JOUR
T1 - Characterization of a novel alphaherpesvirus isolated from the fruit bat Pteropus lylei in Vietnam
AU - Inagaki, Takuya
AU - Yamada, Souichi
AU - Fujii, Hikaru
AU - Yoshikawa, Tomoki
AU - Shibamura, Miho
AU - Harada, Shizuko
AU - Fukushi, Shuetsu
AU - Le, Mai Quynh
AU - Nguyen, Co Thach
AU - Nguyen, Thi Thu Thuy
AU - Nguyen, Thanh Thuy
AU - Nguyen, Thanh Thuy
AU - Quach, Van Tay
AU - Thong, Vu Dinh
AU - Mori, Kazuki
AU - Sasaki, Michihito
AU - Setiyono, Agus
AU - Handharyani, Ekowati
AU - Takeyama, Haruko
AU - Hasebe, Futoshi
AU - Saijo, Masayuki
N1 - Funding Information:
This work was funded by a Grant-in-Aid for Scientific Research from the Ministry of Education, Culture, Sports, Science and Technology (grant number 18K07894) and by the Japan Agency for Medical Research and Development under grant numbers JP19fm0108001 and JP20wm0125006 (Japan Initiative for Global Research Network on Infectious Diseases). We declare that there are no conflicts of interest with regard to this work.
Funding Information:
We thank Hirofumi Sawa (Hokkaido University), Ken Maeda (Yamaguchi University), and Yasushi Kawaguchi (University of Tokyo) for providing us with PtAHV1, the FBKT1 cell line, and HSV-1 F and HSV-2 186, respectively. We thank Yoshiko Fukui and Mihoko Tsuda for their assistance with this study. This work was funded by a Grant-in-Aid for Scientific Research from the Ministry of Education, Culture, Sports, Science and Technology (grant number 18K07894) and by the Japan Agency for Medical Research and Development under grant numbers JP19fm0108001 and JP20wm0125006 (Japan Initiative for Global Research Network on Infectious Diseases). We declare that there are no conflicts of interest with regard to this work.
Publisher Copyright:
Copyright © 2020 American Society for Microbiology. All Rights Reserved.
PY - 2020/9/1
Y1 - 2020/9/1
N2 - Herpesviruses exist in nature within each host animal. Ten herpesviruses have been isolated from bats and their biological properties reported. A novel bat alphaherpesvirus, which we propose to name “Pteropus lylei-associated alphaherpesvirus (PLAHV),” was isolated from urine of the fruit bat Pteropus lylei in Vietnam and characterized. The entire genome sequence was determined to be 144,008 bp in length and predicted to include 72 genes. PLAHV was assigned to genus Simplexvirus with other bat alphaherpesviruses isolated from pteropodid bats in Southeast Asia and Africa. The replication capacity of PLAHV in several cells was evaluated in comparison with that of herpes simplex virus 1 (HSV-1). PLAHV replicated better in the bat-originated cell line and less in human embryonic lung fibroblasts than HSV-1 did. PLAHV was serologically related to another bat alphaherpesvirus, Pteropodid alphaherpesvirus 1 (PtAHV1), isolated from a Pteropus hypomelanus-related bat captured in Indonesia, but not with HSV-1. PLAHV caused lethal infection in mice. PLAHV was as susceptible to acyclovir as HSV-1 was. Characterization of this new member of bat alphaherpesviruses, PLAHV, expands the knowledge on bat-associated alphaherpesvirology. IMPORTANCE A novel bat alphaherpesvirus, Pteropus lylei-associated alphaherpesvirus (PLAHV), was isolated from urine of the fruit bat Pteropus lylei in Vietnam. The whole-genome sequence was determined and was predicted to include 72 open reading frames in the 144,008-bp genome. PLAHV is circulating in a species of fruit bats, Pteropus lylei, in Asia. This study expands the knowledge on bat-associated alphaherpesvirology.
AB - Herpesviruses exist in nature within each host animal. Ten herpesviruses have been isolated from bats and their biological properties reported. A novel bat alphaherpesvirus, which we propose to name “Pteropus lylei-associated alphaherpesvirus (PLAHV),” was isolated from urine of the fruit bat Pteropus lylei in Vietnam and characterized. The entire genome sequence was determined to be 144,008 bp in length and predicted to include 72 genes. PLAHV was assigned to genus Simplexvirus with other bat alphaherpesviruses isolated from pteropodid bats in Southeast Asia and Africa. The replication capacity of PLAHV in several cells was evaluated in comparison with that of herpes simplex virus 1 (HSV-1). PLAHV replicated better in the bat-originated cell line and less in human embryonic lung fibroblasts than HSV-1 did. PLAHV was serologically related to another bat alphaherpesvirus, Pteropodid alphaherpesvirus 1 (PtAHV1), isolated from a Pteropus hypomelanus-related bat captured in Indonesia, but not with HSV-1. PLAHV caused lethal infection in mice. PLAHV was as susceptible to acyclovir as HSV-1 was. Characterization of this new member of bat alphaherpesviruses, PLAHV, expands the knowledge on bat-associated alphaherpesvirology. IMPORTANCE A novel bat alphaherpesvirus, Pteropus lylei-associated alphaherpesvirus (PLAHV), was isolated from urine of the fruit bat Pteropus lylei in Vietnam. The whole-genome sequence was determined and was predicted to include 72 open reading frames in the 144,008-bp genome. PLAHV is circulating in a species of fruit bats, Pteropus lylei, in Asia. This study expands the knowledge on bat-associated alphaherpesvirology.
KW - Bat alphaherpesvirus
KW - Pteropus lylei
KW - Vietnam
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U2 - 10.1128/JVI.00673-20
DO - 10.1128/JVI.00673-20
M3 - Article
C2 - 32669329
AN - SCOPUS:85090176332
SN - 0022-538X
VL - 94
JO - Journal of Virology
JF - Journal of Virology
IS - 18
M1 - e00673-20
ER -