Characterization of a novel gene involved in cadmium accumulation screened from sponge-associated bacterial metagenome

Tetsushi Mori, Koji Iwamoto, Satoshi Wakaoji, Hiroya Araie, Yotaro Kohara, Yoshiko Okamura, Yoshihiro Shiraiwa, Haruko Takeyama

    Research output: Contribution to journalArticle

    6 Citations (Scopus)

    Abstract

    Metagenome research has brought much attention for the identification of important and novel genes of industrial and pharmaceutical value. Here, using a metagenome library constructed from bacteria associated with the marine sponge, Styllisa massa, a high-throughput screening technique using radioisotope was implemented to screen for cadmium (Cd) binding or accumulation genes. From a total of 3301 randomly selected clones, a clone 247-11C was identified as harboring an open reading frame (ORF) showing Cd accumulation characteristics. The ORF, termed as ORF5, was further analyzed by protein functional studies to reveal the presence of a protein, Cdae-1. Cdae-1, composed of a signal peptide and domain harboring an E(G/A)KCG pentapeptide motif, enhanced Cd accumulation when expressed in Escherichia coli. Although showing no direct binding to Cd in vitro, the presence of important amino acid residues related to Cd detoxification suggests that Cdae-1 may possess a different mechanism from known Cd binding proteins such as metallothioneins (MTs) and phytochelatins (PCs). In summary, using the advantage of bacterial metagenomes, our findings in this work suggest the first report on the identification of a unique protein involved in Cd accumulation from bacteria associated with a marine sponge.

    Original languageEnglish
    Pages (from-to)618-625
    Number of pages8
    JournalGene
    Volume576
    Issue number2
    DOIs
    Publication statusPublished - 2016 Feb 1

    Fingerprint

    Metagenome
    Porifera
    Cadmium
    Genes
    Open Reading Frames
    Phytochelatins
    Clone Cells
    Bacteria
    Proteins
    Metallothionein
    Protein Sorting Signals
    Radioisotopes
    Escherichia coli
    Amino Acids
    Research
    Pharmaceutical Preparations

    Keywords

    • Cd accumulation
    • Pentapeptide motif
    • Sponge-associated bacterial metagenome

    ASJC Scopus subject areas

    • Genetics

    Cite this

    Characterization of a novel gene involved in cadmium accumulation screened from sponge-associated bacterial metagenome. / Mori, Tetsushi; Iwamoto, Koji; Wakaoji, Satoshi; Araie, Hiroya; Kohara, Yotaro; Okamura, Yoshiko; Shiraiwa, Yoshihiro; Takeyama, Haruko.

    In: Gene, Vol. 576, No. 2, 01.02.2016, p. 618-625.

    Research output: Contribution to journalArticle

    Mori, T, Iwamoto, K, Wakaoji, S, Araie, H, Kohara, Y, Okamura, Y, Shiraiwa, Y & Takeyama, H 2016, 'Characterization of a novel gene involved in cadmium accumulation screened from sponge-associated bacterial metagenome', Gene, vol. 576, no. 2, pp. 618-625. https://doi.org/10.1016/j.gene.2015.10.018
    Mori, Tetsushi ; Iwamoto, Koji ; Wakaoji, Satoshi ; Araie, Hiroya ; Kohara, Yotaro ; Okamura, Yoshiko ; Shiraiwa, Yoshihiro ; Takeyama, Haruko. / Characterization of a novel gene involved in cadmium accumulation screened from sponge-associated bacterial metagenome. In: Gene. 2016 ; Vol. 576, No. 2. pp. 618-625.
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