Characterization of a putative promoter region of the human poly(ADP-ribose) polymerase gene: Structural similarity to that of the DNA polymerase β gene

Tsutomu Ogura, Hiroshi Nyunoya, Mitsuko Takahashi-Masutani, Masanao Miwa, Takashi Sugimura, Hiroyasu Esumi

Research output: Contribution to journalArticle

36 Citations (Scopus)

Abstract

The 5′-flanking region of the human poly(ADP-ribose) polymerase gene was isolated and characterized. The nucleotide sequence of a part of the poly(ADP-ribose) polymerase gene completely matched that of the cDNA. The transcriptional initiation sites (cap sites) of this gene, located about 166-bp upstream from the translational initiation site, were identified by S1 mapping analysis. Neither CAAT box nor TATA box was found within 500-bp upstream from the cap sites of poly(ADP-ribose) polymerase gene. The 200-bp immediately upstream of the cap site had a high G+C content (76.5%) and contained double repeats of the sequence CCGCCC, putative Sp1 binding sites, and a palindromic structure. The 5′-flanking region of poly(ADP-ribose) polymerase gene also showed promoter activity in chloramphenicol acetyltransferase assay and structural similarity to that of DNA polymerase β gene.

Original languageEnglish
Pages (from-to)701-710
Number of pages10
JournalBiochemical and Biophysical Research Communications
Volume167
Issue number2
DOIs
Publication statusPublished - 1990 Mar 16
Externally publishedYes

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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