Characterization of a staurosporine- and temperature-sensitive mutant, stt1, of Saccharomyces cerevisiae: STT1 is allelic to PKC1

Satoshi Yoshida, Eri Ikeda, Isao Uno, Hiroshi Mitsuzawa

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Abstract

Staurosporine is an antibiotic that specifically inhibits protein kinase C. Fourteen staurosporine- and temperature-sensitive (stt) mutants of Saccharomyces cerevisiae were isolated and characterized. These mutants were divided into ten complementation groups, and characterized for their cross-sensitivity to K-252a, neomycin, or CaCl2, The STT1 gene was cloned and sequenced. The nucleotide sequence of the STT1 gene revealed that STT1 is the same gene as PKC1. The STT1 gene conferred resistance to staurosporine on wild-type cells, when present on a high copy number plasmid. STT1/stt1::HIS3 diploid cells were more sensitive to staurosporine than STT1/STT1 diploid cells. Analysis of temperature-sensitive stt1 mutants showed that the STT1 gene product functioned in S or G2/M phase. These results suggest that a protein kinase (the STT1 gene product) is one of the essential targets of staurosporine in yeast cells.

Original languageEnglish
Pages (from-to)337-344
Number of pages8
JournalMGG Molecular & General Genetics
Volume231
Issue number3
DOIs
Publication statusPublished - 1992 Feb 1

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Keywords

  • Cell cycle
  • Protein kinase C
  • Saccharomyces cerevisiae
  • Staurosporine

ASJC Scopus subject areas

  • Genetics

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