Characterization of aldehyde ferredoxin oxidoreductase gene defective mutant in Magnetospirillum magneticum AMB-1

Aris Tri Wahyudi, Haruko Takeyama, Yoshiko Okamura, Yorikane Fukuda, Tadashi Matsunaga

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

A non-magnetic mutant of Magnetospirillum magneticum AMB-1, designated as NMA21, was generated by mini-Tn5 transposon mutagenesis to identify genes involved in bacterial magnetic particle (BMP) synthesis. Alignment of the DNA sequences flanking the transposon allowed the isolation of an open reading frame (ORF2) within an operon consisting of five genes. The amino acid sequence of ORF2 showed homology with tungsten-containing aldehyde ferredoxin oxidoreductase (AOR) from Pyrococcus furiosus (48% identity and 64% similarity), which functions for aldehyde oxidation. AOR was found to be expressed under microaerobic conditions and localized in the cytoplasm of AMB-1. Iron uptake and growth of NMA21 were lower than wild type. Transmission electron microscopy (TEM) of NMA21 revealed that no BMPs were completely synthesized, but polyhydroxybutyrate (PHB)-like granules were persistently produced. These results indicate that AOR may contribute to ferric iron reduction during BMP synthesis in M. magneticum AMB-1 under microaerobic respiration.

Original languageEnglish
Pages (from-to)223-229
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume303
Issue number1
DOIs
Publication statusPublished - 2003 Mar 28
Externally publishedYes

Fingerprint

Magnetospirillum
Genes
Iron
Pyrococcus furiosus
Mutagenesis
Tungsten
DNA sequences
Operon
Transmission Electron Microscopy
Aldehydes
Open Reading Frames
Amino Acid Sequence
Respiration
Cytoplasm
Transmission electron microscopy
Amino Acids
Oxidation
Growth
aldehyde ferredoxin oxidoreductase

Keywords

  • Aldehyde ferredoxin oxidoreductase
  • Bacterial magnetic particle
  • Magnetospirillum magneticum AMB-1
  • Non-magnetic mutant

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Characterization of aldehyde ferredoxin oxidoreductase gene defective mutant in Magnetospirillum magneticum AMB-1. / Wahyudi, Aris Tri; Takeyama, Haruko; Okamura, Yoshiko; Fukuda, Yorikane; Matsunaga, Tadashi.

In: Biochemical and Biophysical Research Communications, Vol. 303, No. 1, 28.03.2003, p. 223-229.

Research output: Contribution to journalArticle

Wahyudi, Aris Tri ; Takeyama, Haruko ; Okamura, Yoshiko ; Fukuda, Yorikane ; Matsunaga, Tadashi. / Characterization of aldehyde ferredoxin oxidoreductase gene defective mutant in Magnetospirillum magneticum AMB-1. In: Biochemical and Biophysical Research Communications. 2003 ; Vol. 303, No. 1. pp. 223-229.
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