Characterization of amyloid β protein species in cerebral amyloid angiopathy of a squirrel monkey by immunocytochemistry and enzyme-linked immunosorbent assay

Naoya Sawamura, Akira Tamaoka, ShiN'Ichi Shoji, Edward H. Koo, Lary C. Walker, Hiroshi Mori

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

We analyzed the composition of amyloid β protein (A β) species in cerebral amyloid angiopathy (CAA) of an aged squirrel monkey. Immunocytochemistry demonstrated that the cerebral cortex contained no lesions other than widespread CAA with A β40 as its apparent major component. However, enzyme-linked immunosorbent assay revealed that A β42(43) predominated over A β40 in a formic acid-extracted cortical fraction. These findings suggest possible underestimation of A β42(43) levels in some previous immunocytochemical investigations.

Original languageEnglish
Pages (from-to)225-229
Number of pages5
JournalBrain Research
Volume764
Issue number1-2
DOIs
Publication statusPublished - 1997 Aug 1
Externally publishedYes

Fingerprint

Cerebral Amyloid Angiopathy
Amyloidogenic Proteins
Saimiri
formic acid
Enzyme-Linked Immunosorbent Assay
Immunohistochemistry
Serum Amyloid A Protein
Cerebral Cortex

Keywords

  • Alzheimer's disease
  • Amyloid β protein
  • Cerebral amyloid angiopathy
  • Enzyme-linked immunosorbent assay
  • Immunocytochemistry
  • Squirrel monkey

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Characterization of amyloid β protein species in cerebral amyloid angiopathy of a squirrel monkey by immunocytochemistry and enzyme-linked immunosorbent assay. / Sawamura, Naoya; Tamaoka, Akira; Shoji, ShiN'Ichi; Koo, Edward H.; Walker, Lary C.; Mori, Hiroshi.

In: Brain Research, Vol. 764, No. 1-2, 01.08.1997, p. 225-229.

Research output: Contribution to journalArticle

Sawamura, Naoya ; Tamaoka, Akira ; Shoji, ShiN'Ichi ; Koo, Edward H. ; Walker, Lary C. ; Mori, Hiroshi. / Characterization of amyloid β protein species in cerebral amyloid angiopathy of a squirrel monkey by immunocytochemistry and enzyme-linked immunosorbent assay. In: Brain Research. 1997 ; Vol. 764, No. 1-2. pp. 225-229.
@article{a3a24a657920496a9e997bfd2cc9b582,
title = "Characterization of amyloid β protein species in cerebral amyloid angiopathy of a squirrel monkey by immunocytochemistry and enzyme-linked immunosorbent assay",
abstract = "We analyzed the composition of amyloid β protein (A β) species in cerebral amyloid angiopathy (CAA) of an aged squirrel monkey. Immunocytochemistry demonstrated that the cerebral cortex contained no lesions other than widespread CAA with A β40 as its apparent major component. However, enzyme-linked immunosorbent assay revealed that A β42(43) predominated over A β40 in a formic acid-extracted cortical fraction. These findings suggest possible underestimation of A β42(43) levels in some previous immunocytochemical investigations.",
keywords = "Alzheimer's disease, Amyloid β protein, Cerebral amyloid angiopathy, Enzyme-linked immunosorbent assay, Immunocytochemistry, Squirrel monkey",
author = "Naoya Sawamura and Akira Tamaoka and ShiN'Ichi Shoji and Koo, {Edward H.} and Walker, {Lary C.} and Hiroshi Mori",
year = "1997",
month = "8",
day = "1",
doi = "10.1016/S0006-8993(97)00624-0",
language = "English",
volume = "764",
pages = "225--229",
journal = "Brain Research",
issn = "0006-8993",
publisher = "Elsevier",
number = "1-2",

}

TY - JOUR

T1 - Characterization of amyloid β protein species in cerebral amyloid angiopathy of a squirrel monkey by immunocytochemistry and enzyme-linked immunosorbent assay

AU - Sawamura, Naoya

AU - Tamaoka, Akira

AU - Shoji, ShiN'Ichi

AU - Koo, Edward H.

AU - Walker, Lary C.

AU - Mori, Hiroshi

PY - 1997/8/1

Y1 - 1997/8/1

N2 - We analyzed the composition of amyloid β protein (A β) species in cerebral amyloid angiopathy (CAA) of an aged squirrel monkey. Immunocytochemistry demonstrated that the cerebral cortex contained no lesions other than widespread CAA with A β40 as its apparent major component. However, enzyme-linked immunosorbent assay revealed that A β42(43) predominated over A β40 in a formic acid-extracted cortical fraction. These findings suggest possible underestimation of A β42(43) levels in some previous immunocytochemical investigations.

AB - We analyzed the composition of amyloid β protein (A β) species in cerebral amyloid angiopathy (CAA) of an aged squirrel monkey. Immunocytochemistry demonstrated that the cerebral cortex contained no lesions other than widespread CAA with A β40 as its apparent major component. However, enzyme-linked immunosorbent assay revealed that A β42(43) predominated over A β40 in a formic acid-extracted cortical fraction. These findings suggest possible underestimation of A β42(43) levels in some previous immunocytochemical investigations.

KW - Alzheimer's disease

KW - Amyloid β protein

KW - Cerebral amyloid angiopathy

KW - Enzyme-linked immunosorbent assay

KW - Immunocytochemistry

KW - Squirrel monkey

UR - http://www.scopus.com/inward/record.url?scp=0030852418&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0030852418&partnerID=8YFLogxK

U2 - 10.1016/S0006-8993(97)00624-0

DO - 10.1016/S0006-8993(97)00624-0

M3 - Article

C2 - 9295214

AN - SCOPUS:0030852418

VL - 764

SP - 225

EP - 229

JO - Brain Research

JF - Brain Research

SN - 0006-8993

IS - 1-2

ER -