Characterization of antioxidant protection of cultured neural progenitor cells (NPC) against methylmercury (MeHg) toxicity

Jun Watanabe, Tomoya Nakamachi, Tetsuo Ogawa, Akira Naganuma, Masahisa Nakamura, Seiji Shioda, Sigeo Nakajo

    Research output: Contribution to journalArticle

    22 Citations (Scopus)

    Abstract

    Methylmercury (MeHg) is an environmental pollutant known to cause neurobehavioral defects and is especially toxic to the developing brain. With recent studies showing that fetal exposure to low-dose MeHg causes developmental abnormalities, it is therefore important to find ways to combat its effects as well as to clarify the mechanism(s) underlying MeHg toxicity. In the present study, the effects of MeHg on cultured neural progenitor cells (NPC) derived from mouse embryonic brain were investigated. We first confirmed the vulnerability of embryonic NPC to MeHg toxicity, NPC from the telencephalon were more sensitive to MeHg compared to those from the diencephalon. Buthionine sulfoximine (BSO) which is known to inhibit glutathione synthesis accelerated MeHg toxicity. Furthermore, antioxidants such as N-acetyl cysteine and α-tocopherol dramatically rescued the NPC from MeHg's toxic effects. Interestingly, a 12 hr delay in the addition of either antioxidant was still able to prevent the cells from undergoing cell death. Although it is now difficult to avoid MeHg exposure from our environment and contaminated foods, taking anti-oxidants from foods or supplements may prevent or diminish the toxicological effects of MeHg.

    Original languageEnglish
    Pages (from-to)315-325
    Number of pages11
    JournalJournal of Toxicological Sciences
    Volume34
    Issue number3
    DOIs
    Publication statusPublished - 2009 Jun

    Fingerprint

    Toxicity
    Stem Cells
    Antioxidants
    Poisons
    Brain
    Acetylcysteine
    Buthionine Sulfoximine
    Environmental Pollutants
    Tocopherols
    Cell death
    Oxidants
    Diencephalon
    Telencephalon
    Glutathione
    Dietary Supplements
    Toxicology
    Cysteine
    Defects
    Cell Death
    Food

    Keywords

    • Antioxidant
    • Cell death
    • Methylmercury
    • Neural progenitor cell
    • Oxidative stress

    ASJC Scopus subject areas

    • Toxicology

    Cite this

    Characterization of antioxidant protection of cultured neural progenitor cells (NPC) against methylmercury (MeHg) toxicity. / Watanabe, Jun; Nakamachi, Tomoya; Ogawa, Tetsuo; Naganuma, Akira; Nakamura, Masahisa; Shioda, Seiji; Nakajo, Sigeo.

    In: Journal of Toxicological Sciences, Vol. 34, No. 3, 06.2009, p. 315-325.

    Research output: Contribution to journalArticle

    Watanabe, J, Nakamachi, T, Ogawa, T, Naganuma, A, Nakamura, M, Shioda, S & Nakajo, S 2009, 'Characterization of antioxidant protection of cultured neural progenitor cells (NPC) against methylmercury (MeHg) toxicity', Journal of Toxicological Sciences, vol. 34, no. 3, pp. 315-325. https://doi.org/10.2131/jts.34.315
    Watanabe, Jun ; Nakamachi, Tomoya ; Ogawa, Tetsuo ; Naganuma, Akira ; Nakamura, Masahisa ; Shioda, Seiji ; Nakajo, Sigeo. / Characterization of antioxidant protection of cultured neural progenitor cells (NPC) against methylmercury (MeHg) toxicity. In: Journal of Toxicological Sciences. 2009 ; Vol. 34, No. 3. pp. 315-325.
    @article{14b5286f20284598a222cef7595d1869,
    title = "Characterization of antioxidant protection of cultured neural progenitor cells (NPC) against methylmercury (MeHg) toxicity",
    abstract = "Methylmercury (MeHg) is an environmental pollutant known to cause neurobehavioral defects and is especially toxic to the developing brain. With recent studies showing that fetal exposure to low-dose MeHg causes developmental abnormalities, it is therefore important to find ways to combat its effects as well as to clarify the mechanism(s) underlying MeHg toxicity. In the present study, the effects of MeHg on cultured neural progenitor cells (NPC) derived from mouse embryonic brain were investigated. We first confirmed the vulnerability of embryonic NPC to MeHg toxicity, NPC from the telencephalon were more sensitive to MeHg compared to those from the diencephalon. Buthionine sulfoximine (BSO) which is known to inhibit glutathione synthesis accelerated MeHg toxicity. Furthermore, antioxidants such as N-acetyl cysteine and α-tocopherol dramatically rescued the NPC from MeHg's toxic effects. Interestingly, a 12 hr delay in the addition of either antioxidant was still able to prevent the cells from undergoing cell death. Although it is now difficult to avoid MeHg exposure from our environment and contaminated foods, taking anti-oxidants from foods or supplements may prevent or diminish the toxicological effects of MeHg.",
    keywords = "Antioxidant, Cell death, Methylmercury, Neural progenitor cell, Oxidative stress",
    author = "Jun Watanabe and Tomoya Nakamachi and Tetsuo Ogawa and Akira Naganuma and Masahisa Nakamura and Seiji Shioda and Sigeo Nakajo",
    year = "2009",
    month = "6",
    doi = "10.2131/jts.34.315",
    language = "English",
    volume = "34",
    pages = "315--325",
    journal = "Journal of Toxicological Sciences",
    issn = "0388-1350",
    publisher = "Japanese Society of Toxicological Sciences",
    number = "3",

    }

    TY - JOUR

    T1 - Characterization of antioxidant protection of cultured neural progenitor cells (NPC) against methylmercury (MeHg) toxicity

    AU - Watanabe, Jun

    AU - Nakamachi, Tomoya

    AU - Ogawa, Tetsuo

    AU - Naganuma, Akira

    AU - Nakamura, Masahisa

    AU - Shioda, Seiji

    AU - Nakajo, Sigeo

    PY - 2009/6

    Y1 - 2009/6

    N2 - Methylmercury (MeHg) is an environmental pollutant known to cause neurobehavioral defects and is especially toxic to the developing brain. With recent studies showing that fetal exposure to low-dose MeHg causes developmental abnormalities, it is therefore important to find ways to combat its effects as well as to clarify the mechanism(s) underlying MeHg toxicity. In the present study, the effects of MeHg on cultured neural progenitor cells (NPC) derived from mouse embryonic brain were investigated. We first confirmed the vulnerability of embryonic NPC to MeHg toxicity, NPC from the telencephalon were more sensitive to MeHg compared to those from the diencephalon. Buthionine sulfoximine (BSO) which is known to inhibit glutathione synthesis accelerated MeHg toxicity. Furthermore, antioxidants such as N-acetyl cysteine and α-tocopherol dramatically rescued the NPC from MeHg's toxic effects. Interestingly, a 12 hr delay in the addition of either antioxidant was still able to prevent the cells from undergoing cell death. Although it is now difficult to avoid MeHg exposure from our environment and contaminated foods, taking anti-oxidants from foods or supplements may prevent or diminish the toxicological effects of MeHg.

    AB - Methylmercury (MeHg) is an environmental pollutant known to cause neurobehavioral defects and is especially toxic to the developing brain. With recent studies showing that fetal exposure to low-dose MeHg causes developmental abnormalities, it is therefore important to find ways to combat its effects as well as to clarify the mechanism(s) underlying MeHg toxicity. In the present study, the effects of MeHg on cultured neural progenitor cells (NPC) derived from mouse embryonic brain were investigated. We first confirmed the vulnerability of embryonic NPC to MeHg toxicity, NPC from the telencephalon were more sensitive to MeHg compared to those from the diencephalon. Buthionine sulfoximine (BSO) which is known to inhibit glutathione synthesis accelerated MeHg toxicity. Furthermore, antioxidants such as N-acetyl cysteine and α-tocopherol dramatically rescued the NPC from MeHg's toxic effects. Interestingly, a 12 hr delay in the addition of either antioxidant was still able to prevent the cells from undergoing cell death. Although it is now difficult to avoid MeHg exposure from our environment and contaminated foods, taking anti-oxidants from foods or supplements may prevent or diminish the toxicological effects of MeHg.

    KW - Antioxidant

    KW - Cell death

    KW - Methylmercury

    KW - Neural progenitor cell

    KW - Oxidative stress

    UR - http://www.scopus.com/inward/record.url?scp=67649208814&partnerID=8YFLogxK

    UR - http://www.scopus.com/inward/citedby.url?scp=67649208814&partnerID=8YFLogxK

    U2 - 10.2131/jts.34.315

    DO - 10.2131/jts.34.315

    M3 - Article

    VL - 34

    SP - 315

    EP - 325

    JO - Journal of Toxicological Sciences

    JF - Journal of Toxicological Sciences

    SN - 0388-1350

    IS - 3

    ER -