Characterization of antisera directed against follistatin/activin-binding protein peptides

S. Saito, K. Sugino, K. Yamanouchi, K. Kogawa, K. Titani, K. Shiota, M. Takahashi, H. Sugino

Research output: Contribution to journalArticle

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Abstract

An attempt was made to develop immunologic probes directed against follistatin/activin-binding protein (ABP), for use in investigating the distribution of ABP in various tissues. Five oligopeptides corresponding to different regions of the predicted ABP amino acid sequence (peptides 1-12, 28-43, 123-134, 270-281 and 300-315) were synthesized chemically, and coupled to Limulus polyphemus hemolymph hemocyanin. The peptide-hemocyanin conjugates were then used to immunize rabbits, and the immunoresponses were monitored by enzyme-linked immunosorbent assay. Reactivity of the antipeptide antisera with ABP was determined by SDS-polyacrylamide gel electrophoresis and immunoblotting analysis. All of the peptides produced immune responses. The antiserum to peptide 123-134 recognized all forms of ABP, whereas the antiserum to peptide 300-315 reacted specifically and sensitively with the long forms of ABP. These two antisera exhibited only a limited cross-reaction with other proteins or none at all. Therefore, they will be useful for studying the distribution of ABP in various tissues.

Original languageEnglish
Pages (from-to)377-382
Number of pages6
JournalEndocrinologia Japonica
Volume38
Issue number4
Publication statusPublished - 1991
Externally publishedYes

Fingerprint

Follistatin
Immune Sera
Peptides
Hemocyanin
Horseshoe Crabs
Oligopeptides
Hemolymph
Cross Reactions
Immunoblotting
Polyacrylamide Gel Electrophoresis
Amino Acid Sequence
Enzyme-Linked Immunosorbent Assay
Rabbits

ASJC Scopus subject areas

  • Endocrinology

Cite this

Saito, S., Sugino, K., Yamanouchi, K., Kogawa, K., Titani, K., Shiota, K., ... Sugino, H. (1991). Characterization of antisera directed against follistatin/activin-binding protein peptides. Endocrinologia Japonica, 38(4), 377-382.

Characterization of antisera directed against follistatin/activin-binding protein peptides. / Saito, S.; Sugino, K.; Yamanouchi, K.; Kogawa, K.; Titani, K.; Shiota, K.; Takahashi, M.; Sugino, H.

In: Endocrinologia Japonica, Vol. 38, No. 4, 1991, p. 377-382.

Research output: Contribution to journalArticle

Saito, S, Sugino, K, Yamanouchi, K, Kogawa, K, Titani, K, Shiota, K, Takahashi, M & Sugino, H 1991, 'Characterization of antisera directed against follistatin/activin-binding protein peptides', Endocrinologia Japonica, vol. 38, no. 4, pp. 377-382.
Saito S, Sugino K, Yamanouchi K, Kogawa K, Titani K, Shiota K et al. Characterization of antisera directed against follistatin/activin-binding protein peptides. Endocrinologia Japonica. 1991;38(4):377-382.
Saito, S. ; Sugino, K. ; Yamanouchi, K. ; Kogawa, K. ; Titani, K. ; Shiota, K. ; Takahashi, M. ; Sugino, H. / Characterization of antisera directed against follistatin/activin-binding protein peptides. In: Endocrinologia Japonica. 1991 ; Vol. 38, No. 4. pp. 377-382.
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