Characterization of antisera directed against follistatin/activin-binding protein peptides

S. Saito, K. Sugino, K. Yamanouchi, K. Kogawa, K. Titani, K. Shiota, M. Takahashi, H. Sugino

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

An attempt was made to develop immunologic probes directed against follistatin/activin-binding protein (ABP), for use in investigating the distribution of ABP in various tissues. Five oligopeptides corresponding to different regions of the predicted ABP amino acid sequence (peptides 1-12, 28-43, 123-134, 270-281 and 300-315) were synthesized chemically, and coupled to Limulus polyphemus hemolymph hemocyanin. The peptide-hemocyanin conjugates were then used to immunize rabbits, and the immunoresponses were monitored by enzyme-linked immunosorbent assay. Reactivity of the antipeptide antisera with ABP was determined by SDS-polyacrylamide gel electrophoresis and immunoblotting analysis. All of the peptides produced immune responses. The antiserum to peptide 123-134 recognized all forms of ABP, whereas the antiserum to peptide 300-315 reacted specifically and sensitively with the long forms of ABP. These two antisera exhibited only a limited cross-reaction with other proteins or none at all. Therefore, they will be useful for studying the distribution of ABP in various tissues.

Original languageEnglish
Pages (from-to)377-382
Number of pages6
JournalEndocrinologia Japonica
Volume38
Issue number4
Publication statusPublished - 1991
Externally publishedYes

    Fingerprint

ASJC Scopus subject areas

  • Endocrinology

Cite this

Saito, S., Sugino, K., Yamanouchi, K., Kogawa, K., Titani, K., Shiota, K., Takahashi, M., & Sugino, H. (1991). Characterization of antisera directed against follistatin/activin-binding protein peptides. Endocrinologia Japonica, 38(4), 377-382.