Characterization of denitrifying polyphosphate-accumulating organisms in activated sludge based on nitrite reductase gene

Satoshi Tsuneda, Ryuki Miyauchi, Takashi Ohno, Akira Hirata

    Research output: Contribution to journalArticle

    43 Citations (Scopus)

    Abstract

    Nitrite reductase gene (nirS) fragments in the activated sludge obtained from a sequencing batch reactor (SBR) under anaerobic-aerobic condition were cloned and classified by restriction fragment length polymorphism (RFLP) analysis, and representative fragments were sequenced. One of the nirS clones was approximately 70% of all nirS clones in anaerobic/aerobic (existing oxygen and nitrate) cycle operation in which a large amount of anoxic phosphate uptake was observed. Although the activated sludge samples analyzed might contain bacteria that did not accumulate polyphosphate, it was likely that this nirS fragment sequence was that from denitrifying polyphosphate-accumulating organisms (DNPAOs) which can utilize both oxygen and nitrate as electron acceptors. The sequence was similar to the nirS sequences of Thauera mechernichensis (83% similarity) and Azoarcus tolulyticus (83% similarity) both of which belong to the Rhodocyclus group.

    Original languageEnglish
    Pages (from-to)403-407
    Number of pages5
    JournalJournal of Bioscience and Bioengineering
    Volume99
    Issue number4
    DOIs
    Publication statusPublished - 2005 Apr

    Fingerprint

    Nitrite Reductases
    Polyphosphates
    Sewage
    Nitrates
    Rhodocyclaceae
    Thauera
    Azoarcus
    Clone Cells
    Genes
    Oxygen
    Batch reactors
    Polymorphism
    Restriction Fragment Length Polymorphisms
    Bacteria
    Phosphates
    Electrons
    Oxidoreductases

    Keywords

    • Activated sludge
    • Denitrifying polyphosphate-accumulating organisms (DNPAOs)
    • Enhanced biological phosphorus removal (EBPR)
    • Nitrite reductase gene (nirS)
    • Polymerase chain reaction (PCR)
    • Polyphosphate-accumulating organisms (PAOs)

    ASJC Scopus subject areas

    • Biotechnology
    • Bioengineering

    Cite this

    Characterization of denitrifying polyphosphate-accumulating organisms in activated sludge based on nitrite reductase gene. / Tsuneda, Satoshi; Miyauchi, Ryuki; Ohno, Takashi; Hirata, Akira.

    In: Journal of Bioscience and Bioengineering, Vol. 99, No. 4, 04.2005, p. 403-407.

    Research output: Contribution to journalArticle

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