Characterization of DNA hybridization on partially aminated diamond by aromatic compounds

Jung Hoon Yang, Kwang Soup Song, Guo Jun Zhang, Munenori Degawa, Yoshinori Sasaki, Iwao Ohdomari, Hiroshi Kawarada

Research output: Contribution to journalArticle

39 Citations (Scopus)

Abstract

Here, we report a novel method of micropatterning oligonucleotides via aromatic groups as linkers on partially amino-terminated diamond and the inherence on subsequent hybridization. The covalent immobilization of probe oligonucleotides and characterization of immobilized probe oligonucleotides with carboxylic compounds were investigated by X-ray photoelectron spectroscopy (XPS). To confirm the effects of linker flexibility in a low amino group on diamond for probe oligonucleotides, three kinds of dicarboxylic compound - adipic acid, terephthalic acid, and trimesic acid - were used for immobilization of probe oligonucleotides, like linkers; and these oligonucleotides were hybridized with target oligonucleotides labeled with Cy 5 on the micropatterned diamond surface. The hybridization intensities determined by epifluorescence microscopy were compared and analyzed.

Original languageEnglish
Pages (from-to)11245-11250
Number of pages6
JournalLangmuir
Volume22
Issue number26
DOIs
Publication statusPublished - 2006 Dec 19

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ASJC Scopus subject areas

  • Materials Science(all)
  • Condensed Matter Physics
  • Surfaces and Interfaces
  • Spectroscopy
  • Electrochemistry

Cite this

Yang, J. H., Song, K. S., Zhang, G. J., Degawa, M., Sasaki, Y., Ohdomari, I., & Kawarada, H. (2006). Characterization of DNA hybridization on partially aminated diamond by aromatic compounds. Langmuir, 22(26), 11245-11250. https://doi.org/10.1021/la060677l