Characterization of DNA hybridization on partially aminated diamond by aromatic compounds

Jung Hoon Yang, Kwang Soup Song, Guo Jun Zhang, Munenori Degawa, Yoshinori Sasaki, Iwao Ohdomari, Hiroshi Kawarada

    Research output: Contribution to journalArticle

    39 Citations (Scopus)

    Abstract

    Here, we report a novel method of micropatterning oligonucleotides via aromatic groups as linkers on partially amino-terminated diamond and the inherence on subsequent hybridization. The covalent immobilization of probe oligonucleotides and characterization of immobilized probe oligonucleotides with carboxylic compounds were investigated by X-ray photoelectron spectroscopy (XPS). To confirm the effects of linker flexibility in a low amino group on diamond for probe oligonucleotides, three kinds of dicarboxylic compound - adipic acid, terephthalic acid, and trimesic acid - were used for immobilization of probe oligonucleotides, like linkers; and these oligonucleotides were hybridized with target oligonucleotides labeled with Cy 5 on the micropatterned diamond surface. The hybridization intensities determined by epifluorescence microscopy were compared and analyzed.

    Original languageEnglish
    Pages (from-to)11245-11250
    Number of pages6
    JournalLangmuir
    Volume22
    Issue number26
    DOIs
    Publication statusPublished - 2006 Dec 19

    Fingerprint

    Diamond
    Oligonucleotide Probes
    oligonucleotides
    aromatic compounds
    Aromatic compounds
    Oligonucleotides
    Diamonds
    DNA
    deoxyribonucleic acid
    diamonds
    Immobilized Nucleic Acids
    probes
    immobilization
    acids
    Acids
    Microscopic examination
    X ray photoelectron spectroscopy
    flexibility
    photoelectron spectroscopy
    microscopy

    ASJC Scopus subject areas

    • Physical and Theoretical Chemistry
    • Colloid and Surface Chemistry

    Cite this

    Yang, J. H., Song, K. S., Zhang, G. J., Degawa, M., Sasaki, Y., Ohdomari, I., & Kawarada, H. (2006). Characterization of DNA hybridization on partially aminated diamond by aromatic compounds. Langmuir, 22(26), 11245-11250. https://doi.org/10.1021/la060677l

    Characterization of DNA hybridization on partially aminated diamond by aromatic compounds. / Yang, Jung Hoon; Song, Kwang Soup; Zhang, Guo Jun; Degawa, Munenori; Sasaki, Yoshinori; Ohdomari, Iwao; Kawarada, Hiroshi.

    In: Langmuir, Vol. 22, No. 26, 19.12.2006, p. 11245-11250.

    Research output: Contribution to journalArticle

    Yang, JH, Song, KS, Zhang, GJ, Degawa, M, Sasaki, Y, Ohdomari, I & Kawarada, H 2006, 'Characterization of DNA hybridization on partially aminated diamond by aromatic compounds', Langmuir, vol. 22, no. 26, pp. 11245-11250. https://doi.org/10.1021/la060677l
    Yang JH, Song KS, Zhang GJ, Degawa M, Sasaki Y, Ohdomari I et al. Characterization of DNA hybridization on partially aminated diamond by aromatic compounds. Langmuir. 2006 Dec 19;22(26):11245-11250. https://doi.org/10.1021/la060677l
    Yang, Jung Hoon ; Song, Kwang Soup ; Zhang, Guo Jun ; Degawa, Munenori ; Sasaki, Yoshinori ; Ohdomari, Iwao ; Kawarada, Hiroshi. / Characterization of DNA hybridization on partially aminated diamond by aromatic compounds. In: Langmuir. 2006 ; Vol. 22, No. 26. pp. 11245-11250.
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