Characterization of extracellular RNAs produced by the marine photosynthetic bacterium Rhodovulum sulfidophilum

Tomoaki Ando, Hiromichi Suzuki, Seiichiro Nishimura, Terumichi Tanaka, Akira Hiraishi, Yo Kikuchi

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

The marine photosynthetic bacterium Rhodovulum sulfidophilum produces extracellular nucleic acids that are involved in its flocculation. These were found to be produced concomitantly with cell growth. The RNA fraction of these extracellular nucleic acids was subjected to cDNA analysis by applying a micro RNA cloning method and found to contain mainly fully mature-sized tRNAs and fragments of 16S and 23S rRNAs. Analyses of modified bases and genes of the RNAs revealed no structural difference between the intracellular and extracellular RNAs. This is the first report of structural analyses of bacterial extracellular RNAs.

Original languageEnglish
Pages (from-to)805-811
Number of pages7
JournalJournal of Biochemistry
Volume139
Issue number4
DOIs
Publication statusPublished - 2006 Apr
Externally publishedYes

Fingerprint

Rhodovulum
Bacteria
RNA
Nucleic Acids
Bacterial RNA
Flocculation
Transfer RNA
MicroRNAs
Organism Cloning
Cloning
Cell growth
Complementary DNA
Genes
Growth

Keywords

  • Extracellular RNA
  • Flocculation
  • Photosynthetic bacterium
  • Rhodovulum sulfidophilum
  • RNA cloning

ASJC Scopus subject areas

  • Biochemistry

Cite this

Characterization of extracellular RNAs produced by the marine photosynthetic bacterium Rhodovulum sulfidophilum. / Ando, Tomoaki; Suzuki, Hiromichi; Nishimura, Seiichiro; Tanaka, Terumichi; Hiraishi, Akira; Kikuchi, Yo.

In: Journal of Biochemistry, Vol. 139, No. 4, 04.2006, p. 805-811.

Research output: Contribution to journalArticle

Ando, T, Suzuki, H, Nishimura, S, Tanaka, T, Hiraishi, A & Kikuchi, Y 2006, 'Characterization of extracellular RNAs produced by the marine photosynthetic bacterium Rhodovulum sulfidophilum', Journal of Biochemistry, vol. 139, no. 4, pp. 805-811. https://doi.org/10.1093/jb/mvj091
Ando, Tomoaki ; Suzuki, Hiromichi ; Nishimura, Seiichiro ; Tanaka, Terumichi ; Hiraishi, Akira ; Kikuchi, Yo. / Characterization of extracellular RNAs produced by the marine photosynthetic bacterium Rhodovulum sulfidophilum. In: Journal of Biochemistry. 2006 ; Vol. 139, No. 4. pp. 805-811.
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