Abstract
The aim of this work is to elucidate the iron uptake mechanism in the magnetic bacterium Aquaspirillum sp. AMB-1, which can utilize a wide variety of iron chelates. However, no siderophore could be detected in spent culture. A 400-bp DNA fragment was amplified by polymerase chain reaction (PCR) from the genomic DNA of AMB-1, using primers designed from the sfuC gene of Serratia marcescens. This gene encodes a nucleotide binding protein in the periplasmic binding protein (PBP) dependent iron transport system. The amplified fragment was homologous with the conserved sequence of sfuC. Our results suggest that a nucleotide binding protein mediated iron transport system similar to that observed in Serratia marcescens is present in Aquaspirillum sp. AMB-1.
Original language | English |
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Pages (from-to) | 169-176 |
Number of pages | 8 |
Journal | Applied Biochemistry and Biotechnology |
Volume | 39-40 |
Issue number | 1 |
DOIs | |
Publication status | Published - 1993 Sep |
Externally published | Yes |
Keywords
- Aquaspirillum
- Biomineralization
- iron uptake
- magnetite
- nucleotide binding protein
ASJC Scopus subject areas
- Bioengineering
- Biotechnology
- Biochemistry
- Biochemistry, Genetics and Molecular Biology(all)