Abstract
The aim of this work is to elucidate the iron uptake mechanism in the magnetic bacterium Aquaspirillum sp. AMB-1, which can utilize a wide variety of iron chelates. However, no siderophore could be detected in spent culture. A 400-bp DNA fragment was amplified by polymerase chain reaction (PCR) from the genomic DNA of AMB-1, using primers designed from the sfuC gene of Serratia marcescens. This gene encodes a nucleotide binding protein in the periplasmic binding protein (PBP) dependent iron transport system. The amplified fragment was homologous with the conserved sequence of sfuC. Our results suggest that a nucleotide binding protein mediated iron transport system similar to that observed in Serratia marcescens is present in Aquaspirillum sp. AMB-1.
| Original language | English |
|---|---|
| Pages (from-to) | 169-176 |
| Number of pages | 8 |
| Journal | Applied Biochemistry and Biotechnology |
| Volume | 39-40 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - 1993 Sep |
| Externally published | Yes |
Fingerprint
Keywords
- Aquaspirillum
- Biomineralization
- iron uptake
- magnetite
- nucleotide binding protein
ASJC Scopus subject areas
- Bioengineering
- Biotechnology
- Biochemistry
- Biochemistry, Genetics and Molecular Biology(all)
Cite this
Characterization of iron uptake in the magnetic bacterium Aquaspirillum sp. AMB-1. / Nakamura, Chikashi; Sakaguchi, Toshifumi; Kudo, Satoko; Burgess, J. Grant; Sode, Koji; Matsunaga, Tadashi.
In: Applied Biochemistry and Biotechnology, Vol. 39-40, No. 1, 09.1993, p. 169-176.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Characterization of iron uptake in the magnetic bacterium Aquaspirillum sp. AMB-1
AU - Nakamura, Chikashi
AU - Sakaguchi, Toshifumi
AU - Kudo, Satoko
AU - Burgess, J. Grant
AU - Sode, Koji
AU - Matsunaga, Tadashi
PY - 1993/9
Y1 - 1993/9
N2 - The aim of this work is to elucidate the iron uptake mechanism in the magnetic bacterium Aquaspirillum sp. AMB-1, which can utilize a wide variety of iron chelates. However, no siderophore could be detected in spent culture. A 400-bp DNA fragment was amplified by polymerase chain reaction (PCR) from the genomic DNA of AMB-1, using primers designed from the sfuC gene of Serratia marcescens. This gene encodes a nucleotide binding protein in the periplasmic binding protein (PBP) dependent iron transport system. The amplified fragment was homologous with the conserved sequence of sfuC. Our results suggest that a nucleotide binding protein mediated iron transport system similar to that observed in Serratia marcescens is present in Aquaspirillum sp. AMB-1.
AB - The aim of this work is to elucidate the iron uptake mechanism in the magnetic bacterium Aquaspirillum sp. AMB-1, which can utilize a wide variety of iron chelates. However, no siderophore could be detected in spent culture. A 400-bp DNA fragment was amplified by polymerase chain reaction (PCR) from the genomic DNA of AMB-1, using primers designed from the sfuC gene of Serratia marcescens. This gene encodes a nucleotide binding protein in the periplasmic binding protein (PBP) dependent iron transport system. The amplified fragment was homologous with the conserved sequence of sfuC. Our results suggest that a nucleotide binding protein mediated iron transport system similar to that observed in Serratia marcescens is present in Aquaspirillum sp. AMB-1.
KW - Aquaspirillum
KW - Biomineralization
KW - iron uptake
KW - magnetite
KW - nucleotide binding protein
UR - http://www.scopus.com/inward/record.url?scp=0002657104&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0002657104&partnerID=8YFLogxK
U2 - 10.1007/BF02918987
DO - 10.1007/BF02918987
M3 - Article
AN - SCOPUS:0002657104
VL - 39-40
SP - 169
EP - 176
JO - Applied Biochemistry and Biotechnology
JF - Applied Biochemistry and Biotechnology
SN - 0273-2289
IS - 1
ER -