Characterization of iron uptake in the magnetic bacterium Aquaspirillum sp. AMB-1

Chikashi Nakamura; Toshifumi Sakaguchi; Satoko Kudo; J. Grant Burgess; Koji Sode; Tadashi Matsunaga

doi:10.1007/BF02918987

Characterization of iron uptake in the magnetic bacterium Aquaspirillum sp. AMB-1

Chikashi Nakamura, Toshifumi Sakaguchi, Satoko Kudo, J. Grant Burgess, Koji Sode, Tadashi Matsunaga

Research output: Contribution to journal › Article › peer-review

16 Citations (Scopus)

Abstract

The aim of this work is to elucidate the iron uptake mechanism in the magnetic bacterium Aquaspirillum sp. AMB-1, which can utilize a wide variety of iron chelates. However, no siderophore could be detected in spent culture. A 400-bp DNA fragment was amplified by polymerase chain reaction (PCR) from the genomic DNA of AMB-1, using primers designed from the sfuC gene of Serratia marcescens. This gene encodes a nucleotide binding protein in the periplasmic binding protein (PBP) dependent iron transport system. The amplified fragment was homologous with the conserved sequence of sfuC. Our results suggest that a nucleotide binding protein mediated iron transport system similar to that observed in Serratia marcescens is present in Aquaspirillum sp. AMB-1.

Original language	English
Pages (from-to)	169-176
Number of pages	8
Journal	Applied Biochemistry and Biotechnology
Volume	39-40
Issue number	1
DOIs	https://doi.org/10.1007/BF02918987
Publication status	Published - 1993 Sep
Externally published	Yes

Keywords

Aquaspirillum
Biomineralization
iron uptake
magnetite
nucleotide binding protein

ASJC Scopus subject areas

Bioengineering
Biotechnology
Biochemistry
Biochemistry, Genetics and Molecular Biology(all)

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10.1007/BF02918987

Cite this

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abstract = "The aim of this work is to elucidate the iron uptake mechanism in the magnetic bacterium Aquaspirillum sp. AMB-1, which can utilize a wide variety of iron chelates. However, no siderophore could be detected in spent culture. A 400-bp DNA fragment was amplified by polymerase chain reaction (PCR) from the genomic DNA of AMB-1, using primers designed from the sfuC gene of Serratia marcescens. This gene encodes a nucleotide binding protein in the periplasmic binding protein (PBP) dependent iron transport system. The amplified fragment was homologous with the conserved sequence of sfuC. Our results suggest that a nucleotide binding protein mediated iron transport system similar to that observed in Serratia marcescens is present in Aquaspirillum sp. AMB-1.",

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author = "Chikashi Nakamura and Toshifumi Sakaguchi and Satoko Kudo and Burgess, {J. Grant} and Koji Sode and Tadashi Matsunaga",

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AU - Nakamura, Chikashi

AU - Sakaguchi, Toshifumi

AU - Kudo, Satoko

AU - Burgess, J. Grant

AU - Sode, Koji

AU - Matsunaga, Tadashi

PY - 1993/9

Y1 - 1993/9

N2 - The aim of this work is to elucidate the iron uptake mechanism in the magnetic bacterium Aquaspirillum sp. AMB-1, which can utilize a wide variety of iron chelates. However, no siderophore could be detected in spent culture. A 400-bp DNA fragment was amplified by polymerase chain reaction (PCR) from the genomic DNA of AMB-1, using primers designed from the sfuC gene of Serratia marcescens. This gene encodes a nucleotide binding protein in the periplasmic binding protein (PBP) dependent iron transport system. The amplified fragment was homologous with the conserved sequence of sfuC. Our results suggest that a nucleotide binding protein mediated iron transport system similar to that observed in Serratia marcescens is present in Aquaspirillum sp. AMB-1.

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KW - Aquaspirillum

KW - Biomineralization

KW - iron uptake

KW - magnetite

KW - nucleotide binding protein

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Characterization of iron uptake in the magnetic bacterium Aquaspirillum sp. AMB-1

Abstract

Keywords

ASJC Scopus subject areas

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