Characterization of iron uptake in the magnetic bacterium Aquaspirillum sp. AMB-1

Chikashi Nakamura, Toshifumi Sakaguchi, Satoko Kudo, J. Grant Burgess, Koji Sode, Tadashi Matsunaga

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

The aim of this work is to elucidate the iron uptake mechanism in the magnetic bacterium Aquaspirillum sp. AMB-1, which can utilize a wide variety of iron chelates. However, no siderophore could be detected in spent culture. A 400-bp DNA fragment was amplified by polymerase chain reaction (PCR) from the genomic DNA of AMB-1, using primers designed from the sfuC gene of Serratia marcescens. This gene encodes a nucleotide binding protein in the periplasmic binding protein (PBP) dependent iron transport system. The amplified fragment was homologous with the conserved sequence of sfuC. Our results suggest that a nucleotide binding protein mediated iron transport system similar to that observed in Serratia marcescens is present in Aquaspirillum sp. AMB-1.

Original languageEnglish
Pages (from-to)169-176
Number of pages8
JournalApplied Biochemistry and Biotechnology
Volume39-40
Issue number1
DOIs
Publication statusPublished - 1993 Sep
Externally publishedYes

Fingerprint

Serratia marcescens
Bacteria
Nucleotides
Iron
Periplasmic Binding Proteins
Iron-Binding Proteins
Iron Chelating Agents
Siderophores
Conserved Sequence
DNA
Carrier Proteins
Genes
Polymerase chain reaction
Polymerase Chain Reaction

Keywords

  • Aquaspirillum
  • Biomineralization
  • iron uptake
  • magnetite
  • nucleotide binding protein

ASJC Scopus subject areas

  • Bioengineering
  • Biotechnology
  • Biochemistry
  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Nakamura, C., Sakaguchi, T., Kudo, S., Burgess, J. G., Sode, K., & Matsunaga, T. (1993). Characterization of iron uptake in the magnetic bacterium Aquaspirillum sp. AMB-1. Applied Biochemistry and Biotechnology, 39-40(1), 169-176. https://doi.org/10.1007/BF02918987

Characterization of iron uptake in the magnetic bacterium Aquaspirillum sp. AMB-1. / Nakamura, Chikashi; Sakaguchi, Toshifumi; Kudo, Satoko; Burgess, J. Grant; Sode, Koji; Matsunaga, Tadashi.

In: Applied Biochemistry and Biotechnology, Vol. 39-40, No. 1, 09.1993, p. 169-176.

Research output: Contribution to journalArticle

Nakamura, C, Sakaguchi, T, Kudo, S, Burgess, JG, Sode, K & Matsunaga, T 1993, 'Characterization of iron uptake in the magnetic bacterium Aquaspirillum sp. AMB-1', Applied Biochemistry and Biotechnology, vol. 39-40, no. 1, pp. 169-176. https://doi.org/10.1007/BF02918987
Nakamura, Chikashi ; Sakaguchi, Toshifumi ; Kudo, Satoko ; Burgess, J. Grant ; Sode, Koji ; Matsunaga, Tadashi. / Characterization of iron uptake in the magnetic bacterium Aquaspirillum sp. AMB-1. In: Applied Biochemistry and Biotechnology. 1993 ; Vol. 39-40, No. 1. pp. 169-176.
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