Characterization of iron uptake in the magnetic bacterium Aquaspirillum sp. AMB-1

Chikashi Nakamura; Toshifumi Sakaguchi; Satoko Kudo; J. Grant Burgess; Koji Sode; Tadashi Matsunaga

doi:10.1007/BF02918987

Characterization of iron uptake in the magnetic bacterium Aquaspirillum sp. AMB-1

Chikashi Nakamura, Toshifumi Sakaguchi, Satoko Kudo, J. Grant Burgess, Koji Sode, Tadashi Matsunaga

Research output: Contribution to journal › Article

16 Citations (Scopus)

Abstract

The aim of this work is to elucidate the iron uptake mechanism in the magnetic bacterium Aquaspirillum sp. AMB-1, which can utilize a wide variety of iron chelates. However, no siderophore could be detected in spent culture. A 400-bp DNA fragment was amplified by polymerase chain reaction (PCR) from the genomic DNA of AMB-1, using primers designed from the sfuC gene of Serratia marcescens. This gene encodes a nucleotide binding protein in the periplasmic binding protein (PBP) dependent iron transport system. The amplified fragment was homologous with the conserved sequence of sfuC. Our results suggest that a nucleotide binding protein mediated iron transport system similar to that observed in Serratia marcescens is present in Aquaspirillum sp. AMB-1.

Original language	English
Pages (from-to)	169-176
Number of pages	8
Journal	Applied Biochemistry and Biotechnology
Volume	39-40
Issue number	1
DOIs	https://doi.org/10.1007/BF02918987
Publication status	Published - 1993 Sep
Externally published	Yes

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Keywords

Aquaspirillum
Biomineralization
iron uptake
magnetite
nucleotide binding protein

ASJC Scopus subject areas

Bioengineering
Biotechnology
Biochemistry
Biochemistry, Genetics and Molecular Biology(all)

Cite this

Nakamura, C., Sakaguchi, T., Kudo, S., Burgess, J. G., Sode, K., & Matsunaga, T. (1993). Characterization of iron uptake in the magnetic bacterium Aquaspirillum sp. AMB-1. Applied Biochemistry and Biotechnology, 39-40(1), 169-176. https://doi.org/10.1007/BF02918987

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10.1007/BF02918987