Characterization of iron uptake in the magnetic bacterium Aquaspirillum sp. AMB-1

Chikashi Nakamura, Toshifumi Sakaguchi, Satoko Kudo, J. Grant Burgess, Koji Sode, Tadashi Matsunaga

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Abstract

The aim of this work is to elucidate the iron uptake mechanism in the magnetic bacterium Aquaspirillum sp. AMB-1, which can utilize a wide variety of iron chelates. However, no siderophore could be detected in spent culture. A 400-bp DNA fragment was amplified by polymerase chain reaction (PCR) from the genomic DNA of AMB-1, using primers designed from the sfuC gene of Serratia marcescens. This gene encodes a nucleotide binding protein in the periplasmic binding protein (PBP) dependent iron transport system. The amplified fragment was homologous with the conserved sequence of sfuC. Our results suggest that a nucleotide binding protein mediated iron transport system similar to that observed in Serratia marcescens is present in Aquaspirillum sp. AMB-1.

Original languageEnglish
Pages (from-to)169-176
Number of pages8
JournalApplied Biochemistry and Biotechnology
Volume39-40
Issue number1
DOIs
Publication statusPublished - 1993 Sep
Externally publishedYes

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Keywords

  • Aquaspirillum
  • Biomineralization
  • iron uptake
  • magnetite
  • nucleotide binding protein

ASJC Scopus subject areas

  • Bioengineering
  • Biotechnology
  • Biochemistry
  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Nakamura, C., Sakaguchi, T., Kudo, S., Burgess, J. G., Sode, K., & Matsunaga, T. (1993). Characterization of iron uptake in the magnetic bacterium Aquaspirillum sp. AMB-1. Applied Biochemistry and Biotechnology, 39-40(1), 169-176. https://doi.org/10.1007/BF02918987