Characterization of magnetic nanoparticles modified with thiol functionalized PAMAM dendron for DNA recovery

Tsuyoshi Tanaka, Keiyu Shibata, Masahito Hosokawa, Keiichi Hatakeyama, Atsushi Arakaki, Hideyuki Gomyo, Takeyuki Mogi, Tomoyuki Taguchi, Hitoshi Wake, Takeo Tanaami, Tadashi Matsunaga

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

Magnetic nanoparticles (MNPs) modified with the thiol functionalized polyamidoamine (PAMAM) dendron were synthesized to estimate their DNA recovery capabilities. Aminosilane-modified MNPs and MNPs surrounded by a phospholipid (distearoylphosphatidylethanolamine (DSPE)) bilayer were used as core particles. Cystamine-core PAMAM dendrimers were reduced by dithiothreitol to dendron thiols and chemically conjugated to the core particles. Characterization of the synthesis revealed an increase of the surface amine charge from generation 1 (G1) to G6, starting with an aminosilane initiator. Particle size distribution analysis indicated that G6 PAMAM-modified MNPs exhibited monodispersity in an aqueous solution. G6 PAMAM-MNPs and G6 PAMAM-PE-MNPs synthesized by the proposed method have equivalent DNA recovery abilities to PAMAM-MNPs prepared by the conventional divergent synthesis method. In optimized conditions, 96% of λDNA was recovered using G6 PAMAM-PE-MNPs. Therefore, the method for preparing PAMAM-MNPs and PAMAM-PE-MNPs proposed in this study will be a novel approach for producing DNA carriers for efficient DNA purification by magnetic separation.

Original languageEnglish
Pages (from-to)469-475
Number of pages7
JournalJournal of Colloid and Interface Science
Volume377
Issue number1
DOIs
Publication statusPublished - 2012 Jul 1
Externally publishedYes

Keywords

  • Dendrimer
  • DNA carrier
  • Magnetic nanoparticle
  • Phospholipid bilayer
  • Polyamidoamine

ASJC Scopus subject areas

  • Surfaces, Coatings and Films
  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Colloid and Surface Chemistry

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    Tanaka, T., Shibata, K., Hosokawa, M., Hatakeyama, K., Arakaki, A., Gomyo, H., Mogi, T., Taguchi, T., Wake, H., Tanaami, T., & Matsunaga, T. (2012). Characterization of magnetic nanoparticles modified with thiol functionalized PAMAM dendron for DNA recovery. Journal of Colloid and Interface Science, 377(1), 469-475. https://doi.org/10.1016/j.jcis.2012.03.039