Characterization of microbial community in nitrogen removal process of metallurgic wastewater by PCR-DGGE

S. Yoshie, N. Noda, T. Miyano, Satoshi Tsuneda, A. Hirata, Y. Inamori

    Research output: Contribution to journalArticle

    9 Citations (Scopus)

    Abstract

    The metallurgic wastewater generated from the processes of recovering precious metals from industrial wastes contains high concentrations of nitrogen compounds such as ammonia and nitric acid and of salts such as sodium chloride and sodium sulfate. Biological nitrogen removal from this wastewater was attempted by a circulating bioreactor system equipped with an anoxic packed bed and an aerobic fluidized bed. The anoxic packed bed of this system was found to effectively remove nitrite and nitrate from the wastewater by denitrification at a removal ratio of 97%. As a result of denitrification activity tests at various NaCl concentrations, the sludge obtained from the anoxic packed bed exhibited accumulation of nitrite at 5.0 and 8.4% NaCl concentrations, suggesting that the reduction of nitrite is the key step in the denitrification pathway under hypersaline conditions. The microbial community analysis by denaturing gradient gel electrophoresis (DGGE) of PCR-amplified 16S ribosomal DNA (rDNA) fragments revealed that the community diversity varied in accordance with water temperature, nitrate-loading rate and ionic strength. When particular major DGGE bands were excised, reamplified and directly sequenced, the dominant species in the anoxic packed bed were affiliated with the beta and gamma subclasses of the class Proteobacteria such as Alcaligenes defragrans and Pseudomonas spp., respectively.

    Original languageEnglish
    Pages (from-to)93-98
    Number of pages6
    JournalWater Science and Technology
    Volume46
    Issue number11-12
    Publication statusPublished - 2002

    Fingerprint

    Nitrogen removal
    Packed beds
    Electrophoresis
    nitrite
    denitrification
    Denitrification
    microbial community
    electrokinesis
    Wastewater
    Gels
    gel
    wastewater
    nitrogen
    nitrate
    Nitrates
    nitrogen compound
    precious metal
    sodium chloride
    nitric acid
    industrial waste

    Keywords

    • 16S rDNA
    • Denitrification
    • Hypersaline
    • Metallurgic wastewater
    • Microbial community
    • PCR-DGGE

    ASJC Scopus subject areas

    • Water Science and Technology

    Cite this

    Characterization of microbial community in nitrogen removal process of metallurgic wastewater by PCR-DGGE. / Yoshie, S.; Noda, N.; Miyano, T.; Tsuneda, Satoshi; Hirata, A.; Inamori, Y.

    In: Water Science and Technology, Vol. 46, No. 11-12, 2002, p. 93-98.

    Research output: Contribution to journalArticle

    Yoshie, S. ; Noda, N. ; Miyano, T. ; Tsuneda, Satoshi ; Hirata, A. ; Inamori, Y. / Characterization of microbial community in nitrogen removal process of metallurgic wastewater by PCR-DGGE. In: Water Science and Technology. 2002 ; Vol. 46, No. 11-12. pp. 93-98.
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