Characterization of neural stem cells and their progeny in the adult zebrafish optic tectum

Yoko Ito, Hideomi Tanaka, Hitoshi Okamoto, Toshio Ohshima

    Research output: Contribution to journalArticle

    69 Citations (Scopus)

    Abstract

    In the adult teleost brain, proliferating cells are observed in a broad area, while these cells have a restricted distribution in adult mammalian brains. In the adult teleost optic tectum, most of the proliferating cells are distributed in the caudal margin of the periventricular gray zone (PGZ). We found that the PGZ is largely divided into 3 regions: 1 mitotic region and 2 post-mitotic regions-the superficial and deep layers. These regions are distinguished by the differential expression of several marker genes: pcna, sox2, msi1, elavl3, gfap, fabp7a, and s100β. Using transgenic zebrafish Tg (gfap:GFP), we found that the deep layer cells specifically express gfap:GFP and have a radial glial morphology. We noted that bromodeoxyuridine (BrdU)-positive cells in the mitotic region did not exhibit glial properties, but maintained neuroepithelial characteristics. Pulse chase experiments with BrdU-positive cells revealed the presence of self-renewing stem cells within the mitotic region. BrdU-positive cells differentiate into glutamatergic or GABAergic neurons and oligodendrocytes in the superficial layer and into radial glial cells in the deep layer. These results demonstrate that the proliferating cells in the PGZ contribute to neuronal and glial lineages to maintain the structure of the optic tectum in adult zebrafish.

    Original languageEnglish
    Pages (from-to)26-38
    Number of pages13
    JournalDevelopmental Biology
    Volume342
    Issue number1
    DOIs
    Publication statusPublished - 2010 Jun

    Fingerprint

    Neural Stem Cells
    Superior Colliculi
    Zebrafish
    Bromodeoxyuridine
    Neuroglia
    Ependymoglial Cells
    GABAergic Neurons
    Oligodendroglia
    Brain
    Stem Cells

    Keywords

    • Adult neurogenesis
    • Neural stem cells
    • Optic tectum
    • Radial glia
    • Zebrafish

    ASJC Scopus subject areas

    • Developmental Biology
    • Cell Biology
    • Molecular Biology

    Cite this

    Characterization of neural stem cells and their progeny in the adult zebrafish optic tectum. / Ito, Yoko; Tanaka, Hideomi; Okamoto, Hitoshi; Ohshima, Toshio.

    In: Developmental Biology, Vol. 342, No. 1, 06.2010, p. 26-38.

    Research output: Contribution to journalArticle

    Ito, Yoko ; Tanaka, Hideomi ; Okamoto, Hitoshi ; Ohshima, Toshio. / Characterization of neural stem cells and their progeny in the adult zebrafish optic tectum. In: Developmental Biology. 2010 ; Vol. 342, No. 1. pp. 26-38.
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