Characterization of peripheral blood T-lymphocytes transduced with HTLV-I Tax mutants with different trans-activating phenotypes

Tsuyoshi Akagi, Hiroaki Ono, Hiroshi Nyunoya, Kunitada Shimotohno

Research output: Contribution to journalArticle

79 Citations (Scopus)

Abstract

Tax1, a transcriptional trans-activator of the Human T-cell leukemia virus type I (HTLV-I), induces the expression of many cellular genes through interaction with at least three distinct cellular transcription factors; CREB/ATF, NF-κB, and SRF. This Tax1-induced activation of cellular genes is considered to be a critical event in T-cell transformation by HTLV-I. To elucidate the role of each Tax1-inducible transcriptional pathway in T-cell transformation, we introduced Tax1 mutants with different trans-activating phenotypes into peripheral blood lymphocytes (PBL) by retroviral vectors. Analysis of these PBLs revealed that activation of the NF-κB pathway is sufficient to promote the growth response to IL-2. However, for the clonal expansion of CD4+ T-cells, which is a characteristic result of HTLV-I infection, activation of the CREB/ATF and SRF pathways is also required.

Original languageEnglish
Pages (from-to)2071-2078
Number of pages8
JournalOncogene
Volume14
Issue number17
DOIs
Publication statusPublished - 1997 Jan 1

Keywords

  • CD4
  • CD8
  • CREB/ATF
  • HTLV-I
  • NF-κB
  • Tax

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Cancer Research

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