TY - JOUR
T1 - Characterization of peripheral blood T-lymphocytes transduced with HTLV-I Tax mutants with different trans-activating phenotypes
AU - Akagi, Tsuyoshi
AU - Ono, Hiroaki
AU - Nyunoya, Hiroshi
AU - Shimotohno, Kunitada
N1 - Funding Information:
We thank Dr M Fujii (Tokyo Medical and Dental University) and Dr M Seiki (Kanazawa University) for providing pCArG-CAT. We also thank Dr WC Greene and Dr J Elwood (University of California San Francisco) for providing the Tax M22 mutant expression vector, and Dr T Sekine (National Cancer Center) for supplying recombinant IL-2. This work was supported in part by Grants-in-Aid for Cancer Research, Grants-in-Aid for a 2nd-term Comprehensive 10-year Strategy for Cancer Control from the Ministry of Health and Welfare of Japan, and Grants-in-Aid from the Ministry of Education, Science and Culture.
PY - 1997
Y1 - 1997
N2 - Tax1, a transcriptional trans-activator of the Human T-cell leukemia virus type I (HTLV-I), induces the expression of many cellular genes through interaction with at least three distinct cellular transcription factors; CREB/ATF, NF-κB, and SRF. This Tax1-induced activation of cellular genes is considered to be a critical event in T-cell transformation by HTLV-I. To elucidate the role of each Tax1-inducible transcriptional pathway in T-cell transformation, we introduced Tax1 mutants with different trans-activating phenotypes into peripheral blood lymphocytes (PBL) by retroviral vectors. Analysis of these PBLs revealed that activation of the NF-κB pathway is sufficient to promote the growth response to IL-2. However, for the clonal expansion of CD4+ T-cells, which is a characteristic result of HTLV-I infection, activation of the CREB/ATF and SRF pathways is also required.
AB - Tax1, a transcriptional trans-activator of the Human T-cell leukemia virus type I (HTLV-I), induces the expression of many cellular genes through interaction with at least three distinct cellular transcription factors; CREB/ATF, NF-κB, and SRF. This Tax1-induced activation of cellular genes is considered to be a critical event in T-cell transformation by HTLV-I. To elucidate the role of each Tax1-inducible transcriptional pathway in T-cell transformation, we introduced Tax1 mutants with different trans-activating phenotypes into peripheral blood lymphocytes (PBL) by retroviral vectors. Analysis of these PBLs revealed that activation of the NF-κB pathway is sufficient to promote the growth response to IL-2. However, for the clonal expansion of CD4+ T-cells, which is a characteristic result of HTLV-I infection, activation of the CREB/ATF and SRF pathways is also required.
KW - CD4
KW - CD8
KW - CREB/ATF
KW - HTLV-I
KW - NF-κB
KW - Tax
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U2 - 10.1038/sj.onc.1201045
DO - 10.1038/sj.onc.1201045
M3 - Article
C2 - 9160887
AN - SCOPUS:0030976361
VL - 14
SP - 2071
EP - 2078
JO - Oncogene
JF - Oncogene
SN - 0950-9232
IS - 17
ER -