Abstract
Tax1, a transcriptional trans-activator of the Human T-cell leukemia virus type I (HTLV-I), induces the expression of many cellular genes through interaction with at least three distinct cellular transcription factors; CREB/ATF, NF-κB, and SRF. This Tax1-induced activation of cellular genes is considered to be a critical event in T-cell transformation by HTLV-I. To elucidate the role of each Tax1-inducible transcriptional pathway in T-cell transformation, we introduced Tax1 mutants with different trans-activating phenotypes into peripheral blood lymphocytes (PBL) by retroviral vectors. Analysis of these PBLs revealed that activation of the NF-κB pathway is sufficient to promote the growth response to IL-2. However, for the clonal expansion of CD4+ T-cells, which is a characteristic result of HTLV-I infection, activation of the CREB/ATF and SRF pathways is also required.
| Original language | English |
|---|---|
| Pages (from-to) | 2071-2078 |
| Number of pages | 8 |
| Journal | Oncogene |
| Volume | 14 |
| Issue number | 17 |
| Publication status | Published - 1997 |
| Externally published | Yes |
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Keywords
- CD4
- CD8
- CREB/ATF
- HTLV-I
- NF-κB
- Tax
ASJC Scopus subject areas
- Cancer Research
- Genetics
- Molecular Biology
Cite this
Characterization of peripheral blood T-lymphocytes transduced with HTLV-I Tax mutants with different trans-activating phenotypes. / Akagi, Tsuyoshi; Ono, Hiroaki; Nyunoya, Hiroshi; Shimotohno, Kunitada.
In: Oncogene, Vol. 14, No. 17, 1997, p. 2071-2078.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Characterization of peripheral blood T-lymphocytes transduced with HTLV-I Tax mutants with different trans-activating phenotypes
AU - Akagi, Tsuyoshi
AU - Ono, Hiroaki
AU - Nyunoya, Hiroshi
AU - Shimotohno, Kunitada
PY - 1997
Y1 - 1997
N2 - Tax1, a transcriptional trans-activator of the Human T-cell leukemia virus type I (HTLV-I), induces the expression of many cellular genes through interaction with at least three distinct cellular transcription factors; CREB/ATF, NF-κB, and SRF. This Tax1-induced activation of cellular genes is considered to be a critical event in T-cell transformation by HTLV-I. To elucidate the role of each Tax1-inducible transcriptional pathway in T-cell transformation, we introduced Tax1 mutants with different trans-activating phenotypes into peripheral blood lymphocytes (PBL) by retroviral vectors. Analysis of these PBLs revealed that activation of the NF-κB pathway is sufficient to promote the growth response to IL-2. However, for the clonal expansion of CD4+ T-cells, which is a characteristic result of HTLV-I infection, activation of the CREB/ATF and SRF pathways is also required.
AB - Tax1, a transcriptional trans-activator of the Human T-cell leukemia virus type I (HTLV-I), induces the expression of many cellular genes through interaction with at least three distinct cellular transcription factors; CREB/ATF, NF-κB, and SRF. This Tax1-induced activation of cellular genes is considered to be a critical event in T-cell transformation by HTLV-I. To elucidate the role of each Tax1-inducible transcriptional pathway in T-cell transformation, we introduced Tax1 mutants with different trans-activating phenotypes into peripheral blood lymphocytes (PBL) by retroviral vectors. Analysis of these PBLs revealed that activation of the NF-κB pathway is sufficient to promote the growth response to IL-2. However, for the clonal expansion of CD4+ T-cells, which is a characteristic result of HTLV-I infection, activation of the CREB/ATF and SRF pathways is also required.
KW - CD4
KW - CD8
KW - CREB/ATF
KW - HTLV-I
KW - NF-κB
KW - Tax
UR - http://www.scopus.com/inward/record.url?scp=0030976361&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0030976361&partnerID=8YFLogxK
M3 - Article
C2 - 9160887
AN - SCOPUS:0030976361
VL - 14
SP - 2071
EP - 2078
JO - Oncogene
JF - Oncogene
SN - 0950-9232
IS - 17
ER -