Characterization of poly(N-isopropylacrylamide) gel grafted polydimethylsiloxane as temperature-responsive cell culture substrate

Yoshikatsu Akiyama, Miki Matsuyama, Naoya Takeda, Masayuki Yamato, Teruo Okano

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Poly(N-isopropylacrylamide) (PIPAAm) hydrogel modified poly(dimethylsiloxane) (PDMS) surface (PIPAAm-PDMS) was prepared as a new temperature-responsive cell culture surface by using electron beam (EB) irradiation method. PIPAAm-PDMS surface properties at unstretched and stretched states were characterized by using various analytical method. As the result, we found that PIPAAm-PDMS surface properties as well as thickness of the grafted PIPAAm gel were altered by applied uniaxial mechanical stretching stress. The mechanical stress can affect the degree of hydration of grafted PIPAAm chains.

Original languageEnglish
Title of host publication2016 International Symposium on Micro-NanoMechatronics and Human Science, MHS 2016
PublisherInstitute of Electrical and Electronics Engineers Inc.
ISBN (Electronic)9781509027842
DOIs
Publication statusPublished - 2017 Jan 18
Externally publishedYes
Event27th International Symposium on Micro-NanoMechatronics and Human Science, MHS 2016 - Nagoya, Japan
Duration: 2016 Nov 282016 Nov 30

Other

Other27th International Symposium on Micro-NanoMechatronics and Human Science, MHS 2016
CountryJapan
CityNagoya
Period16/11/2816/11/30

Fingerprint

Polydimethylsiloxane
Cell culture
Hydrogels
surface properties
Gels
Cell Culture Techniques
gels
Temperature
Surface properties
Substrates
Hydrogel
hydration
electron beams
Mechanical Stress
Surface Properties
Hydration
Stretching
irradiation
temperature
Electron beams

ASJC Scopus subject areas

  • Medicine (miscellaneous)
  • Biomedical Engineering
  • Mechanical Engineering
  • Human-Computer Interaction
  • Instrumentation
  • Computer Science Applications
  • Biotechnology
  • Artificial Intelligence

Cite this

Akiyama, Y., Matsuyama, M., Takeda, N., Yamato, M., & Okano, T. (2017). Characterization of poly(N-isopropylacrylamide) gel grafted polydimethylsiloxane as temperature-responsive cell culture substrate. In 2016 International Symposium on Micro-NanoMechatronics and Human Science, MHS 2016 [7824169] Institute of Electrical and Electronics Engineers Inc.. https://doi.org/10.1109/MHS.2016.7824169

Characterization of poly(N-isopropylacrylamide) gel grafted polydimethylsiloxane as temperature-responsive cell culture substrate. / Akiyama, Yoshikatsu; Matsuyama, Miki; Takeda, Naoya; Yamato, Masayuki; Okano, Teruo.

2016 International Symposium on Micro-NanoMechatronics and Human Science, MHS 2016. Institute of Electrical and Electronics Engineers Inc., 2017. 7824169.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Akiyama, Y, Matsuyama, M, Takeda, N, Yamato, M & Okano, T 2017, Characterization of poly(N-isopropylacrylamide) gel grafted polydimethylsiloxane as temperature-responsive cell culture substrate. in 2016 International Symposium on Micro-NanoMechatronics and Human Science, MHS 2016., 7824169, Institute of Electrical and Electronics Engineers Inc., 27th International Symposium on Micro-NanoMechatronics and Human Science, MHS 2016, Nagoya, Japan, 16/11/28. https://doi.org/10.1109/MHS.2016.7824169
Akiyama Y, Matsuyama M, Takeda N, Yamato M, Okano T. Characterization of poly(N-isopropylacrylamide) gel grafted polydimethylsiloxane as temperature-responsive cell culture substrate. In 2016 International Symposium on Micro-NanoMechatronics and Human Science, MHS 2016. Institute of Electrical and Electronics Engineers Inc. 2017. 7824169 https://doi.org/10.1109/MHS.2016.7824169
Akiyama, Yoshikatsu ; Matsuyama, Miki ; Takeda, Naoya ; Yamato, Masayuki ; Okano, Teruo. / Characterization of poly(N-isopropylacrylamide) gel grafted polydimethylsiloxane as temperature-responsive cell culture substrate. 2016 International Symposium on Micro-NanoMechatronics and Human Science, MHS 2016. Institute of Electrical and Electronics Engineers Inc., 2017.
@inproceedings{977b536f741843b3ac46ed9b72d5e7dc,
title = "Characterization of poly(N-isopropylacrylamide) gel grafted polydimethylsiloxane as temperature-responsive cell culture substrate",
abstract = "Poly(N-isopropylacrylamide) (PIPAAm) hydrogel modified poly(dimethylsiloxane) (PDMS) surface (PIPAAm-PDMS) was prepared as a new temperature-responsive cell culture surface by using electron beam (EB) irradiation method. PIPAAm-PDMS surface properties at unstretched and stretched states were characterized by using various analytical method. As the result, we found that PIPAAm-PDMS surface properties as well as thickness of the grafted PIPAAm gel were altered by applied uniaxial mechanical stretching stress. The mechanical stress can affect the degree of hydration of grafted PIPAAm chains.",
author = "Yoshikatsu Akiyama and Miki Matsuyama and Naoya Takeda and Masayuki Yamato and Teruo Okano",
year = "2017",
month = "1",
day = "18",
doi = "10.1109/MHS.2016.7824169",
language = "English",
booktitle = "2016 International Symposium on Micro-NanoMechatronics and Human Science, MHS 2016",
publisher = "Institute of Electrical and Electronics Engineers Inc.",
address = "United States",

}

TY - GEN

T1 - Characterization of poly(N-isopropylacrylamide) gel grafted polydimethylsiloxane as temperature-responsive cell culture substrate

AU - Akiyama, Yoshikatsu

AU - Matsuyama, Miki

AU - Takeda, Naoya

AU - Yamato, Masayuki

AU - Okano, Teruo

PY - 2017/1/18

Y1 - 2017/1/18

N2 - Poly(N-isopropylacrylamide) (PIPAAm) hydrogel modified poly(dimethylsiloxane) (PDMS) surface (PIPAAm-PDMS) was prepared as a new temperature-responsive cell culture surface by using electron beam (EB) irradiation method. PIPAAm-PDMS surface properties at unstretched and stretched states were characterized by using various analytical method. As the result, we found that PIPAAm-PDMS surface properties as well as thickness of the grafted PIPAAm gel were altered by applied uniaxial mechanical stretching stress. The mechanical stress can affect the degree of hydration of grafted PIPAAm chains.

AB - Poly(N-isopropylacrylamide) (PIPAAm) hydrogel modified poly(dimethylsiloxane) (PDMS) surface (PIPAAm-PDMS) was prepared as a new temperature-responsive cell culture surface by using electron beam (EB) irradiation method. PIPAAm-PDMS surface properties at unstretched and stretched states were characterized by using various analytical method. As the result, we found that PIPAAm-PDMS surface properties as well as thickness of the grafted PIPAAm gel were altered by applied uniaxial mechanical stretching stress. The mechanical stress can affect the degree of hydration of grafted PIPAAm chains.

UR - http://www.scopus.com/inward/record.url?scp=85013682709&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85013682709&partnerID=8YFLogxK

U2 - 10.1109/MHS.2016.7824169

DO - 10.1109/MHS.2016.7824169

M3 - Conference contribution

BT - 2016 International Symposium on Micro-NanoMechatronics and Human Science, MHS 2016

PB - Institute of Electrical and Electronics Engineers Inc.

ER -