characterization of rat mid-pregnancy-specific placental lactogen produced by baculovirus / insect cell expression system

Mitsuko Hirosawa-Takamori, Yoshiharu Matsuura, Satoshi Tanaka, Tomoya Ogawa, Kunio Shiota

Research output: Contribution to journalArticle

Abstract

The placenta produces prolactin (PRL)- or growth hormone-like hormones known as placental lactogens (PLs) which play roles in the maternal endocrine system and fetal growth development. We previously cloned a cDNA encoding a novel rat PL, PL-I mosaic (PL-Im), which is specifically expressed at mid-pregnancy. In this study, the biological activity of PL-Im was evaluated by producing the recombinant protein (recPL-Im) by means of a baculovirus/insect cell expression system. RecPL-Im demonstrated binding activity for PRL receptor in a radioreceptor assay (RRA) system with rat liver membrane fraction and 125I-ovine PRL (oPRL). In addition, the activity of recPL-Im is comparable to oPRL in the Nb2 cell proliferation assay. Interestingly, recPLIm stimulated progesterone secretion in a concentration-dependent manner in a primary culture of luteal cells from pregnant rat, whereas oPRL had no effect on luteal cells. The luteotropic function of recPL-Im was accompanied by suppression of the secretion of 20oc-dihydroprogesterone (20ocOHP) in the culture. PL-Im therefore has ability to bind PRL-receptor and express PRL-like activity. In addition, recPL-Im has luteotropic hormone (LTH) activity and its activity is qualitatively different from that of PRL.

Original languageEnglish
Pages (from-to)43-50
Number of pages8
JournalJournal of Reproduction and Development
Volume45
Issue number1
Publication statusPublished - 1999
Externally publishedYes

Fingerprint

choriomammotropin
Baculoviridae
prolactin
pregnancy
insects
rats
luteal cells
sheep
cells
hormones
secretion
endocrine system
fetal development
assays
placenta
recombinant proteins
somatotropin
bioactive properties
progesterone
cell proliferation

Keywords

  • Corpus lutea
  • Luteotropic
  • Placental lactogen

ASJC Scopus subject areas

  • Animal Science and Zoology

Cite this

characterization of rat mid-pregnancy-specific placental lactogen produced by baculovirus / insect cell expression system. / Hirosawa-Takamori, Mitsuko; Matsuura, Yoshiharu; Tanaka, Satoshi; Ogawa, Tomoya; Shiota, Kunio.

In: Journal of Reproduction and Development, Vol. 45, No. 1, 1999, p. 43-50.

Research output: Contribution to journalArticle

Hirosawa-Takamori, Mitsuko ; Matsuura, Yoshiharu ; Tanaka, Satoshi ; Ogawa, Tomoya ; Shiota, Kunio. / characterization of rat mid-pregnancy-specific placental lactogen produced by baculovirus / insect cell expression system. In: Journal of Reproduction and Development. 1999 ; Vol. 45, No. 1. pp. 43-50.
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