Characterization of soil microflora on a successional glacier foreland in the high Arctic on Ellesmere Island, Nunavut, Canada using phospholipid fatty acid analysis

Shimpei Yoshitake, Masaki Uchida, Takayuki Nakatsubo, Hiroshi Kanda

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

We investigated soil microbial biomass and community structure along a primary successional gradient after deglaciation in the high Arctic, at Ellesmere Island, Nunavut, Canada (80° 50′N, 82° 45′W). Soil samples were collected from five glacial moraines (M1 to M5) with different developmental periods. Time since the recession of glaciers at M1, M3, and M5 was estimated to be 300, 9000, and over 17000 years, respectively. Soil samples from five points in each moraine were subjected to phospholipid fatty acid (PLFA) analysis. Total PLFA content (an index of microbial biomass) in M1 was significantly lower than those in older moraines (M2-M5), whereas the content remained at an almost constant level from M2 to M5. Significant differences in PLFA composition (an index of microbial community structure) were also observed between M1 and older moraines (M2-M5); the proportion of straight chain saturated fatty acids in M1 was higher than those in older moraines (M2-M5), whereas those of branched fatty acids and unsaturated fatty acids in M1 were lower than those in older moraines (M2-M5). These results suggest that changes of microflora occurred in the early phase of succession after deglaciation and became stable thereafter. Microbial biomass had a positive correlation with soil carbon and nitrogen contents over the successional chronosequence, suggesting that development of soil microflora was affected in part by organic matter accumulation.

Original languageEnglish
Pages (from-to)73-84
Number of pages12
JournalPolar Bioscience
Issue number19
Publication statusPublished - 2006 Jan 1
Externally publishedYes

Fingerprint

phospholipid
glacier
fatty acid
soil
deglaciation
biomass
community structure
chronosequence
moraine
soil nitrogen
soil carbon
analysis
microbial community
organic matter

Keywords

  • High Arctic
  • Microbial biomass
  • Microbial community structure
  • Phospholipid fatty acid (PLFA)
  • Primary succession after deglaciation

ASJC Scopus subject areas

  • Environmental Science (miscellaneous)
  • Earth and Planetary Sciences (miscellaneous)

Cite this

Characterization of soil microflora on a successional glacier foreland in the high Arctic on Ellesmere Island, Nunavut, Canada using phospholipid fatty acid analysis. / Yoshitake, Shimpei; Uchida, Masaki; Nakatsubo, Takayuki; Kanda, Hiroshi.

In: Polar Bioscience, No. 19, 01.01.2006, p. 73-84.

Research output: Contribution to journalArticle

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