Characterization of the mouse α1D-adrenergic receptor gene

Kei Arai, Akito Tanoue, Nobuhito Goda, Masayuki Takeda, Kota Takahashi, Gozoh Tsujimoto

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

α1-Adrenergic receptors (α1-ARs) play critical roles in the regulation of a variety of physiological processes. Increasing evidence suggests that multiple receptor subtypes of α1-ARs regulate these physiological processes. Molecular cloning has identified three distinct cDNAs encoding α1-AR subtypes (α1A, α1B and α1D) that are structurally homologous. Among the α1-AR subtypes, the function of the α1D-AR remains unclear. In order to examine the physiological role of α1D-AR, we cloned and characterized a gene for the mouse α1D-AR. Using a mouse α1D-AR cDNA as a probe, we isolated the gene for the mouse α1D-AR from a mouse genomic library. The α1D-AR consists of two exons and an intron that interrupts the coding region of the putative sixth transmembrane domain. The 5'-flanking region of exon 1 contains neither a TATA box nor a CAAT box but is high in GC content and contains several Sp1 binding sites (GC boxes). This pattern is similar to promoters described for other members of α1-ARs. The untranslated region also contains putative cyclic AMP response elements. Isolation of this gene will allow further investigation, via gene knock-outs and deletion mutants, of the mechanisms of transcriptional regulation and a greater understanding of the physiological role of α1D-AR.

Original languageEnglish
Pages (from-to)271-278
Number of pages8
JournalJapanese Journal of Pharmacology
Volume81
Issue number3
DOIs
Publication statusPublished - 1999
Externally publishedYes

Fingerprint

Adrenergic Receptors
Physiological Phenomena
Genes
Exons
Complementary DNA
Untranslated Regions
TATA Box
Genomic Library
5' Flanking Region
Base Composition
Molecular Cloning
Response Elements
Cyclic AMP
Introns
Binding Sites

Keywords

  • α1D-Adrenergic receptor
  • Gene

ASJC Scopus subject areas

  • Pharmacology
  • Molecular Medicine

Cite this

Arai, K., Tanoue, A., Goda, N., Takeda, M., Takahashi, K., & Tsujimoto, G. (1999). Characterization of the mouse α1D-adrenergic receptor gene. Japanese Journal of Pharmacology, 81(3), 271-278. https://doi.org/10.1254/jjp.81.271

Characterization of the mouse α1D-adrenergic receptor gene. / Arai, Kei; Tanoue, Akito; Goda, Nobuhito; Takeda, Masayuki; Takahashi, Kota; Tsujimoto, Gozoh.

In: Japanese Journal of Pharmacology, Vol. 81, No. 3, 1999, p. 271-278.

Research output: Contribution to journalArticle

Arai, K, Tanoue, A, Goda, N, Takeda, M, Takahashi, K & Tsujimoto, G 1999, 'Characterization of the mouse α1D-adrenergic receptor gene', Japanese Journal of Pharmacology, vol. 81, no. 3, pp. 271-278. https://doi.org/10.1254/jjp.81.271
Arai, Kei ; Tanoue, Akito ; Goda, Nobuhito ; Takeda, Masayuki ; Takahashi, Kota ; Tsujimoto, Gozoh. / Characterization of the mouse α1D-adrenergic receptor gene. In: Japanese Journal of Pharmacology. 1999 ; Vol. 81, No. 3. pp. 271-278.
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