Characterization of the mouse α1D-adrenergic receptor gene

Kei Arai, Akito Tanoue, Nobuhito Goda, Masayuki Takeda, Kota Takahashi, Gozoh Tsujimoto

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

α1-Adrenergic receptors (α1-ARs) play critical roles in the regulation of a variety of physiological processes. Increasing evidence suggests that multiple receptor subtypes of α1-ARs regulate these physiological processes. Molecular cloning has identified three distinct cDNAs encoding α1-AR subtypes (α1A, α1B and α1D) that are structurally homologous. Among the α1-AR subtypes, the function of the α1D-AR remains unclear. In order to examine the physiological role of α1D-AR, we cloned and characterized a gene for the mouse α1D-AR. Using a mouse α1D-AR cDNA as a probe, we isolated the gene for the mouse α1D-AR from a mouse genomic library. The α1D-AR consists of two exons and an intron that interrupts the coding region of the putative sixth transmembrane domain. The 5'-flanking region of exon 1 contains neither a TATA box nor a CAAT box but is high in GC content and contains several Sp1 binding sites (GC boxes). This pattern is similar to promoters described for other members of α1-ARs. The untranslated region also contains putative cyclic AMP response elements. Isolation of this gene will allow further investigation, via gene knock-outs and deletion mutants, of the mechanisms of transcriptional regulation and a greater understanding of the physiological role of α1D-AR.

Original languageEnglish
Pages (from-to)271-278
Number of pages8
JournalJapanese Journal of Pharmacology
Volume81
Issue number3
DOIs
Publication statusPublished - 1999 Jan 1

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Keywords

  • Gene
  • α1D-Adrenergic receptor

ASJC Scopus subject areas

  • Pharmacology

Cite this

Arai, K., Tanoue, A., Goda, N., Takeda, M., Takahashi, K., & Tsujimoto, G. (1999). Characterization of the mouse α1D-adrenergic receptor gene. Japanese Journal of Pharmacology, 81(3), 271-278. https://doi.org/10.1254/jjp.81.271