Characterization of the promoter region of the c-yes proto-oncogene

The importance of the GC boxes on its promoter activity

Yumiko Matsuzawa, Kentaro Senba, Junko Kawamura-Tsuzuku, Tatsuhiko Sudo, Shunsuke Ishii, Kumao Toyoshima, Tadashi Yamamoto

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

In this study we cloned the 5′ flanking sequence of the human c-yes gene and identified its promoter region. A 0.53 kilobase pair (kbp) fragment containing the 5′ terminus of the c-yes gene showed strong promoter activity when placed upstream of the bacterial chloramphenicol acetyltransferase (CAT) gene and transfected into monkey CV-1 cells. By nuclease S1 mapping multiple transcriptional start sites were detected within the promoter region. Nucleotide sequence analysis revealed that the c-yes promoter region had high G+C contents (64%) and contained six GC box-like sequences (one at the 5′ distal region and five in a cluster at the 5′ proximal region), but not a TATA box. These features of the c-yes promoter region are similar to those of other protooncogenes, ras-family genes and c-raf-1, and some house-keeping genes. Deletion analysis suggested that the most downstream 0.21 kbp region is primarily important for the promoter activity. This 0.21 kbp region contains one major and another minor transcriptional start site. Five GC box-like sequences were located within this region, and four of them were shown to bind with purified Sp1 transcription factor. Furthermore, using the basesubstituted mutants of the Sp1-binding sites, each GC box in the cluster (GC1 to GC4) was shown to affect the c-yes gene expression.

Original languageEnglish
Pages (from-to)1561-1567
Number of pages7
JournalOncogene
Volume6
Issue number9
Publication statusPublished - 1991 Sep
Externally publishedYes

Fingerprint

Proto-Oncogenes
Genetic Promoter Regions
Sp1 Transcription Factor
Genes
TATA Box
Chloramphenicol O-Acetyltransferase
ras Genes
5' Flanking Region
Essential Genes
Base Composition
Haplorhini
Sequence Analysis
Binding Sites
Gene Expression

ASJC Scopus subject areas

  • Cancer Research
  • Genetics
  • Molecular Biology

Cite this

Matsuzawa, Y., Senba, K., Kawamura-Tsuzuku, J., Sudo, T., Ishii, S., Toyoshima, K., & Yamamoto, T. (1991). Characterization of the promoter region of the c-yes proto-oncogene: The importance of the GC boxes on its promoter activity. Oncogene, 6(9), 1561-1567.

Characterization of the promoter region of the c-yes proto-oncogene : The importance of the GC boxes on its promoter activity. / Matsuzawa, Yumiko; Senba, Kentaro; Kawamura-Tsuzuku, Junko; Sudo, Tatsuhiko; Ishii, Shunsuke; Toyoshima, Kumao; Yamamoto, Tadashi.

In: Oncogene, Vol. 6, No. 9, 09.1991, p. 1561-1567.

Research output: Contribution to journalArticle

Matsuzawa, Y, Senba, K, Kawamura-Tsuzuku, J, Sudo, T, Ishii, S, Toyoshima, K & Yamamoto, T 1991, 'Characterization of the promoter region of the c-yes proto-oncogene: The importance of the GC boxes on its promoter activity', Oncogene, vol. 6, no. 9, pp. 1561-1567.
Matsuzawa Y, Senba K, Kawamura-Tsuzuku J, Sudo T, Ishii S, Toyoshima K et al. Characterization of the promoter region of the c-yes proto-oncogene: The importance of the GC boxes on its promoter activity. Oncogene. 1991 Sep;6(9):1561-1567.
Matsuzawa, Yumiko ; Senba, Kentaro ; Kawamura-Tsuzuku, Junko ; Sudo, Tatsuhiko ; Ishii, Shunsuke ; Toyoshima, Kumao ; Yamamoto, Tadashi. / Characterization of the promoter region of the c-yes proto-oncogene : The importance of the GC boxes on its promoter activity. In: Oncogene. 1991 ; Vol. 6, No. 9. pp. 1561-1567.
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