Characterization of the Promoter Region of the src Family Gene lyn and Its trans Activation by Human T-Cell Leukemia Virus Type I-Encoded p40tax

Fumiaki Uchiumi, Kentaro Senba, Yuji Yamanashi, Jun Ichi Fujisawa, Mitsuaki Yoshida, Kazushi Inoue, Kumao Toyoshima, Tadashi Yamamoto

Research output: Contribution to journalArticle

39 Citations (Scopus)

Abstract

The src family gene lyn is expressed preferentially in B lymphocytes but very little in normal T lymphocytes. Transcription of the lyn gene in T lymphocytes was shown to be induced by the p40tax protein encoded by human T-cell lymphotropic virus type I. For determination of the mechanism of p40tax-mediated trans activation, the transcriptional promoter region of the lyn gene was characterized. By endonuclease S1 mapping, the transcriptional initiation sites were identified within the 770-bp EcoRI-SacI fragment of the 5′-terminal portion of the human lyn gene. This fragment showed promoter activity when placed upstream of the bacterial chloramphenicol acetyltransferase gene and transfected into various cell lines. Nucleotide sequence analysis revealed that the lyn promoter region contained four GC box-like sequences but not a TATA or CCAAT box. In addition, it contained sequences characteristic of a cyclic AMP-responsive element, octamer-binding motif, PEA3-like motifs, and NFκB-binding motif-like sequence. Mutational analysis suggested that the octamer-binding motif sequence is of primary importance for the lyn promoter activity but that the other elements are not. Cotransfection of various chloramphenicol acetyltransferase constructs containing different length of the lyn promoter together with p40tax expression plasmids into Jurkat T cells showed that the sequence responsible for p40tax-induced transcription is present around the transcription initiation sites.

Original languageEnglish
Pages (from-to)3784-3795
Number of pages12
JournalMolecular and Cellular Biology
Volume12
Issue number9
Publication statusPublished - 1992 Sep
Externally publishedYes

Fingerprint

src Genes
Human T-lymphotropic virus 1
Genetic Promoter Regions
T-Lymphocytes
Chloramphenicol O-Acetyltransferase
Genes
Jurkat Cells
Endonucleases
Transcription Initiation Site
Cyclic AMP
Transcriptional Activation
Sequence Analysis
Plasmids
B-Lymphocytes
Viruses
Cell Line
Proteins

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Cell Biology

Cite this

Characterization of the Promoter Region of the src Family Gene lyn and Its trans Activation by Human T-Cell Leukemia Virus Type I-Encoded p40tax . / Uchiumi, Fumiaki; Senba, Kentaro; Yamanashi, Yuji; Fujisawa, Jun Ichi; Yoshida, Mitsuaki; Inoue, Kazushi; Toyoshima, Kumao; Yamamoto, Tadashi.

In: Molecular and Cellular Biology, Vol. 12, No. 9, 09.1992, p. 3784-3795.

Research output: Contribution to journalArticle

Uchiumi, F, Senba, K, Yamanashi, Y, Fujisawa, JI, Yoshida, M, Inoue, K, Toyoshima, K & Yamamoto, T 1992, 'Characterization of the Promoter Region of the src Family Gene lyn and Its trans Activation by Human T-Cell Leukemia Virus Type I-Encoded p40tax ', Molecular and Cellular Biology, vol. 12, no. 9, pp. 3784-3795.
Uchiumi, Fumiaki ; Senba, Kentaro ; Yamanashi, Yuji ; Fujisawa, Jun Ichi ; Yoshida, Mitsuaki ; Inoue, Kazushi ; Toyoshima, Kumao ; Yamamoto, Tadashi. / Characterization of the Promoter Region of the src Family Gene lyn and Its trans Activation by Human T-Cell Leukemia Virus Type I-Encoded p40tax In: Molecular and Cellular Biology. 1992 ; Vol. 12, No. 9. pp. 3784-3795.
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