Chd2 interacts with H3.3 to determine myogenic cell fate

Akihito Harada, Seiji Okada, Daijiro Konno, Jun Odawara, Tomohiko Yoshimi, Saori Yoshimura, Hiromi Kumamaru, Hirokazu Saiwai, Toshiaki Tsubota, Hitoshi Kurumizaka, Koichi Akashi, Taro Tachibana, Anthony N. Imbalzano, Yasuyuki Ohkawa

    Research output: Contribution to journalArticle

    66 Citations (Scopus)

    Abstract

    Cell differentiation is mediated by lineage-determining transcription factors. We show that chromodomain helicase DNA-binding domain 2 (Chd2), a SNF2 chromatin remodelling enzyme family member, interacts with MyoD and myogenic gene regulatory sequences to specifically mark these loci via deposition of the histone variant H3.3 prior to cell differentiation. Directed and genome-wide analysis of endogenous H3.3 incorporation demonstrates that knockdown of Chd2 prevents H3.3 deposition at differentiation-dependent, but not housekeeping, genes and inhibits myogenic gene activation. The data indicate that MyoD determines cell fate and facilitates differentiation-dependent gene expression through Chd2-dependent deposition of H3.3 at myogenic loci prior to differentiation.

    Original languageEnglish
    Pages (from-to)2994-3007
    Number of pages14
    JournalEMBO Journal
    Volume31
    Issue number13
    DOIs
    Publication statusPublished - 2012 Jul 4

    Fingerprint

    DNA Helicases
    Genes
    Cell Differentiation
    Chromatin Assembly and Disassembly
    Essential Genes
    Regulator Genes
    Histones
    Transcriptional Activation
    Transcription Factors
    Gene expression
    Genome
    Chromatin
    Gene Expression
    Chemical activation
    Enzymes

    Keywords

    • chromatin
    • differentiation
    • histone variant
    • myogenesis

    ASJC Scopus subject areas

    • Molecular Biology
    • Biochemistry, Genetics and Molecular Biology(all)
    • Immunology and Microbiology(all)
    • Neuroscience(all)

    Cite this

    Harada, A., Okada, S., Konno, D., Odawara, J., Yoshimi, T., Yoshimura, S., ... Ohkawa, Y. (2012). Chd2 interacts with H3.3 to determine myogenic cell fate. EMBO Journal, 31(13), 2994-3007. https://doi.org/10.1038/emboj.2012.136

    Chd2 interacts with H3.3 to determine myogenic cell fate. / Harada, Akihito; Okada, Seiji; Konno, Daijiro; Odawara, Jun; Yoshimi, Tomohiko; Yoshimura, Saori; Kumamaru, Hiromi; Saiwai, Hirokazu; Tsubota, Toshiaki; Kurumizaka, Hitoshi; Akashi, Koichi; Tachibana, Taro; Imbalzano, Anthony N.; Ohkawa, Yasuyuki.

    In: EMBO Journal, Vol. 31, No. 13, 04.07.2012, p. 2994-3007.

    Research output: Contribution to journalArticle

    Harada, A, Okada, S, Konno, D, Odawara, J, Yoshimi, T, Yoshimura, S, Kumamaru, H, Saiwai, H, Tsubota, T, Kurumizaka, H, Akashi, K, Tachibana, T, Imbalzano, AN & Ohkawa, Y 2012, 'Chd2 interacts with H3.3 to determine myogenic cell fate', EMBO Journal, vol. 31, no. 13, pp. 2994-3007. https://doi.org/10.1038/emboj.2012.136
    Harada A, Okada S, Konno D, Odawara J, Yoshimi T, Yoshimura S et al. Chd2 interacts with H3.3 to determine myogenic cell fate. EMBO Journal. 2012 Jul 4;31(13):2994-3007. https://doi.org/10.1038/emboj.2012.136
    Harada, Akihito ; Okada, Seiji ; Konno, Daijiro ; Odawara, Jun ; Yoshimi, Tomohiko ; Yoshimura, Saori ; Kumamaru, Hiromi ; Saiwai, Hirokazu ; Tsubota, Toshiaki ; Kurumizaka, Hitoshi ; Akashi, Koichi ; Tachibana, Taro ; Imbalzano, Anthony N. ; Ohkawa, Yasuyuki. / Chd2 interacts with H3.3 to determine myogenic cell fate. In: EMBO Journal. 2012 ; Vol. 31, No. 13. pp. 2994-3007.
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