Chemiluminescence enzyme immunoassay using ProteinA-bacterial magnetite complex

Tadashi Matsunaga, Rika Sato, Shinji Kamiya, Tsuyosi Tanaka, Haruko Takeyama

Research output: Contribution to journalConference article

66 Citations (Scopus)

Abstract

Bacterial magnetic particles (BMPs) which have ProteinA expressed on their surface were constructed using magA which is a key gene in BMP biosynthesis in the magnetic bacterium Magnetospirillum sp. AMB-1. Homogenous chemiluminescence enzyme immunoassay using antibody bound ProteinA-BMP complexes was developed for detection of human IgG. A good correlation between the luminescence yield and the concentration of human IgG was obtained in the range of 1-103 ng/ml.

Original languageEnglish
Pages (from-to)126-131
Number of pages6
JournalJournal of Magnetism and Magnetic Materials
Volume194
Issue number1
DOIs
Publication statusPublished - 1999 Apr
EventProceedings of 1998 2nd International Conference on Scientific and Clinical Applications of Magnetic Carriers - Cleveland, OH, USA
Duration: 1998 May 281998 May 30

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ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Condensed Matter Physics

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