Chemiluminescence enzyme immunoassay using ProteinA-bacterial magnetite complex

Tadashi Matsunaga, Rika Sato, Shinji Kamiya, Tsuyosi Tanaka, Haruko Takeyama

Research output: Contribution to journalArticle

66 Citations (Scopus)

Abstract

Bacterial magnetic particles (BMPs) which have ProteinA expressed on their surface were constructed using magA which is a key gene in BMP biosynthesis in the magnetic bacterium Magnetospirillum sp. AMB-1. Homogenous chemiluminescence enzyme immunoassay using antibody bound ProteinA-BMP complexes was developed for detection of human IgG. A good correlation between the luminescence yield and the concentration of human IgG was obtained in the range of 1-103 ng/ml.

Original languageEnglish
Pages (from-to)126-131
Number of pages6
JournalJournal of Magnetism and Magnetic Materials
Volume194
Issue number1
DOIs
Publication statusPublished - 1999 Apr
Externally publishedYes

Fingerprint

Ferrosoferric Oxide
immunoassay
Chemiluminescence
Biosynthesis
chemiluminescence
Magnetite
Antibodies
magnetite
Luminescence
enzymes
Bacteria
Enzymes
Immunoglobulin G
Genes
biosynthesis
antibodies
genes
bacteria
luminescence

ASJC Scopus subject areas

  • Condensed Matter Physics

Cite this

Chemiluminescence enzyme immunoassay using ProteinA-bacterial magnetite complex. / Matsunaga, Tadashi; Sato, Rika; Kamiya, Shinji; Tanaka, Tsuyosi; Takeyama, Haruko.

In: Journal of Magnetism and Magnetic Materials, Vol. 194, No. 1, 04.1999, p. 126-131.

Research output: Contribution to journalArticle

Matsunaga, Tadashi ; Sato, Rika ; Kamiya, Shinji ; Tanaka, Tsuyosi ; Takeyama, Haruko. / Chemiluminescence enzyme immunoassay using ProteinA-bacterial magnetite complex. In: Journal of Magnetism and Magnetic Materials. 1999 ; Vol. 194, No. 1. pp. 126-131.
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