Chemiluminescence Enzyms lmmunoassay Using Bacterial Magnetic Particles

Tadashi Matsunaga, Masashi Kawasaki, Xie Yu, Noriyuki Tsujimura, Noriyuki Nakamura

Research output: Contribution to journalArticle

108 Citations (Scopus)

Abstract

A novel chemiluminescence enzyme immunoassay using bacterial magnetic particles (BMPs) has been developed for highly sensitive and rapid detection of immunoglobulin G. Antibody was immobilized onto BMPs using the heterobifunctional reagents sulfosuccinimidyl 6-[3'-(2-pyridyldithio)propionamido]hexanoate (sulfo-LC-SPDP) and sulfosuccinimidyl 4-(N-maleimidomethyl)cyclohexane-1 -carboxylate (sulfo-SMCC). For the highly sensitive immunoassay method using these BMPs, a good relationship was obtained between the luminescence intensity and mouse IgG concentration in the range of 1-105 fg/mL. Furthermore, in order to reduce assay time and to simplify operations, a rapid chemiluminescence enzyme immunoassay method has been developed. The rapid method was completed in 10 min. A linear relationship was obtained between the luminescence and mouse IgG concentration hi the range of 10-1000 ng/mL,.

Original languageEnglish
Pages (from-to)3551-3554
Number of pages4
JournalAnalytical Chemistry
Volume68
Issue number20
Publication statusPublished - 1996 Oct 15
Externally publishedYes

ASJC Scopus subject areas

  • Analytical Chemistry

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  • Cite this

    Matsunaga, T., Kawasaki, M., Yu, X., Tsujimura, N., & Nakamura, N. (1996). Chemiluminescence Enzyms lmmunoassay Using Bacterial Magnetic Particles. Analytical Chemistry, 68(20), 3551-3554.