Chemokine receptor CCR8 is required for lipopolysaccharide-triggered cytokine production in mouse peritoneal macrophages

Tomoyuki Oshio, Rei Kawashima, Yuki I. Kawamura, Teruki Hagiwara, Noriko Mizutani, Toshihiko Okada, Takeshi Otsubo, Kyoko Inagaki-Ohara, Akihiro Matsukawa, Tatsuya Haga, Shigeru Kakuta, Yoichiro Iwakura, Seijiro Hosokawa, Taeko Dohi

    Research output: Contribution to journalArticle

    13 Citations (Scopus)

    Abstract

    Chemokine (C-C motif) receptor 8 (CCR8), the chemokine receptor for chemokine (C-C motif) ligand 1 (CCL1), is expressed in T-helper type-2 lymphocytes and peritoneal macrophages (PMφ) and is involved in various pathological conditions, including peritoneal adhesions. However, the role of CCR8 in inflammatory responses is not fully elucidated. To investigate the function of CCR8 in macrophages, we compared cytokine secretion from mouse PMφ or bone marrow-derived macrophages (BMMφ) stimulated with various Toll-like receptor (TLR) ligands in CCR8 deficient (CCR8-/-) and wild-type (WT) mice. We found that CCR8-/- PMφ demonstrated attenuated secretion of tumor necrosis factor (TNF)-α, interleukin (IL)-6, and IL-10 when stimulated with lipopolysaccharide (LPS). In particular, LPS-induced IL-10 production absolutely required CCR8. CCR8-dependent cytokine secretion was characteristic of PMφ but not BMMφ. To further investigate this result, we selected the small molecule compound R243 from a library of compounds with CCR8-antagonistic effects on CCL1-induced Ca2+ flux and CCL1-driven PMφ aggregation. Similar to CCR8-/- PMφ, R243 attenuated secretion of TNF-α, IL-6, and most strikingly IL-10 from WT PMφ, but not BMMφ. CCR8-/- PMφ and R243-treated WT PMφ both showed suppressed c-jun N-terminal kinase activity and nuclear factor-κB signaling after LPS treatment when compared with WT PMφ. A c-Jun signaling pathway inhibitor also produced an inhibitory effect on LPS-induced cytokine secretion that was similar to that of CCR8 deficiency or R243 treatment. As seen in CCR8-/- mice, administration of R243 attenuated peritoneal adhesions in vivo. R243 also prevented hapten-induced colitis. These results are indicative of cross talk between signaling pathways downstream of CCR8 and TLR-4 that induces cytokine production by PMφ. Through use of CCR8-/- mice and the new CCR8 inhibitor, R243, we identified a novel macrophage innate immune response pathway that involves a chemokine receptor.

    Original languageEnglish
    Article numbere94445
    JournalPLoS One
    Volume9
    Issue number4
    DOIs
    Publication statusPublished - 2014 Apr 8

    Fingerprint

    CCR Receptors
    Macrophages
    Peritoneal Macrophages
    lipopolysaccharides
    Lipopolysaccharides
    macrophages
    cytokines
    Cytokines
    receptors
    mice
    Interleukin-10
    Ligands
    secretion
    interleukin-10
    bone marrow
    Bone
    Interleukin-6
    chemokine receptors
    tumor necrosis factors
    Chemokine Receptors

    ASJC Scopus subject areas

    • Agricultural and Biological Sciences(all)
    • Biochemistry, Genetics and Molecular Biology(all)
    • Medicine(all)

    Cite this

    Oshio, T., Kawashima, R., Kawamura, Y. I., Hagiwara, T., Mizutani, N., Okada, T., ... Dohi, T. (2014). Chemokine receptor CCR8 is required for lipopolysaccharide-triggered cytokine production in mouse peritoneal macrophages. PLoS One, 9(4), [e94445]. https://doi.org/10.1371/journal.pone.0094445

    Chemokine receptor CCR8 is required for lipopolysaccharide-triggered cytokine production in mouse peritoneal macrophages. / Oshio, Tomoyuki; Kawashima, Rei; Kawamura, Yuki I.; Hagiwara, Teruki; Mizutani, Noriko; Okada, Toshihiko; Otsubo, Takeshi; Inagaki-Ohara, Kyoko; Matsukawa, Akihiro; Haga, Tatsuya; Kakuta, Shigeru; Iwakura, Yoichiro; Hosokawa, Seijiro; Dohi, Taeko.

    In: PLoS One, Vol. 9, No. 4, e94445, 08.04.2014.

    Research output: Contribution to journalArticle

    Oshio, T, Kawashima, R, Kawamura, YI, Hagiwara, T, Mizutani, N, Okada, T, Otsubo, T, Inagaki-Ohara, K, Matsukawa, A, Haga, T, Kakuta, S, Iwakura, Y, Hosokawa, S & Dohi, T 2014, 'Chemokine receptor CCR8 is required for lipopolysaccharide-triggered cytokine production in mouse peritoneal macrophages', PLoS One, vol. 9, no. 4, e94445. https://doi.org/10.1371/journal.pone.0094445
    Oshio, Tomoyuki ; Kawashima, Rei ; Kawamura, Yuki I. ; Hagiwara, Teruki ; Mizutani, Noriko ; Okada, Toshihiko ; Otsubo, Takeshi ; Inagaki-Ohara, Kyoko ; Matsukawa, Akihiro ; Haga, Tatsuya ; Kakuta, Shigeru ; Iwakura, Yoichiro ; Hosokawa, Seijiro ; Dohi, Taeko. / Chemokine receptor CCR8 is required for lipopolysaccharide-triggered cytokine production in mouse peritoneal macrophages. In: PLoS One. 2014 ; Vol. 9, No. 4.
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    AU - Kawashima, Rei

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    AU - Hagiwara, Teruki

    AU - Mizutani, Noriko

    AU - Okada, Toshihiko

    AU - Otsubo, Takeshi

    AU - Inagaki-Ohara, Kyoko

    AU - Matsukawa, Akihiro

    AU - Haga, Tatsuya

    AU - Kakuta, Shigeru

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