Chicken pineal Cry genes: Light-dependent up-regulation of cCry1 and cCry2 transcripts

Kazuyuki Yamamoto, Toshiyuki Okano, Yoshitaka Fukada

Research output: Contribution to journalArticle

62 Citations (Scopus)

Abstract

Vertebrate cryptochrome homologs (CRYs) are negative regulators for the transcription/translation-based autoregulatory feedback loop of the circadian clock. In this study we identified two Cry genes in the chicken, cCry1 and cCry2, which are expressed in the pineal gland. Messenger RNA levels of both cCry1 and cCry2 displayed circadian oscillation in cultured pineal cells under light/dark and constant darkness conditions. Noticeably, their mRNA levels during the light period were significantly higher than those in the dark, indicating light-dependent up-regulation of the two Cry genes mediated by photoreceptor(s) intrinsic to the chick pineal cells. These cCRYs inhibited E-box element-dependent cBMAL1/2-cCLOCK-induced transcription, suggesting that the chick pineal circadian oscillator is composed of molecules that are functionally similar to those of mammals but are subject to light-regulation distinct from the mammalian clockwork.

Original languageEnglish
Pages (from-to)13-16
Number of pages4
JournalNeuroscience Letters
Volume313
Issue number1-2
DOIs
Publication statusPublished - 2001 Nov 2
Externally publishedYes

Fingerprint

Chickens
Up-Regulation
Light
Genes
Cryptochromes
E-Box Elements
Messenger RNA
Circadian Clocks
Pineal Gland
Darkness
Vertebrates
Mammals
Cultured Cells

Keywords

  • Chicken
  • Circadian clock
  • Clock gene
  • Cryptochrome
  • Pineal gland
  • Transcriptional inhibitor

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Chicken pineal Cry genes : Light-dependent up-regulation of cCry1 and cCry2 transcripts. / Yamamoto, Kazuyuki; Okano, Toshiyuki; Fukada, Yoshitaka.

In: Neuroscience Letters, Vol. 313, No. 1-2, 02.11.2001, p. 13-16.

Research output: Contribution to journalArticle

Yamamoto, Kazuyuki ; Okano, Toshiyuki ; Fukada, Yoshitaka. / Chicken pineal Cry genes : Light-dependent up-regulation of cCry1 and cCry2 transcripts. In: Neuroscience Letters. 2001 ; Vol. 313, No. 1-2. pp. 13-16.
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