Chicken red-sensitive cone visual pigment retains a binding domain for transducin

Yoshitaka Fukada, Toshiyuki Okano, Igor D. Artamonov, Tôru Yoshizawa

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

Iodopsin (a red-sensitive cone visual pigment) and rhodopsin (a rod pigment) were isolated from chicken retina. They were separately reconstituted into phosphatidylcholine liposomes and then mixed with rod transducin (Tα and Tβγ) purified from bovine retina. Iodopsin enhanced, only when irradiated, the binding of GppNHp to Tα to a similar extent to irradiated rhodopsin. Furthermore, the binding of GppNHp to Tα in the presence of a photobleaching intermediate of iodopsin preferably required Tβγ-2 rather than Tβγ-1, which is very similar in profile to that in the presence of the intermediate of rhodopsin (J. Biol. Chem., in press). These results indicate that the binding domain for transducin in iodopsin should closely resemble that in rhodopsin.

Original languageEnglish
Pages (from-to)69-72
Number of pages4
JournalFEBS Letters
Volume246
Issue number1-2
DOIs
Publication statusPublished - 1989 Mar 27
Externally publishedYes

Fingerprint

Transducin
Retinal Pigments
Rhodopsin
Cones
Chickens
Retina
Photobleaching
Phosphatidylcholines
Pigments
Liposomes
iodopsin

Keywords

  • (Chicken retina)
  • Cone visual pigment
  • GTP-binding protein
  • Iodopsin
  • Rhodopsin
  • Transducin

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Chicken red-sensitive cone visual pigment retains a binding domain for transducin. / Fukada, Yoshitaka; Okano, Toshiyuki; Artamonov, Igor D.; Yoshizawa, Tôru.

In: FEBS Letters, Vol. 246, No. 1-2, 27.03.1989, p. 69-72.

Research output: Contribution to journalArticle

Fukada, Yoshitaka ; Okano, Toshiyuki ; Artamonov, Igor D. ; Yoshizawa, Tôru. / Chicken red-sensitive cone visual pigment retains a binding domain for transducin. In: FEBS Letters. 1989 ; Vol. 246, No. 1-2. pp. 69-72.
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