CHIP-dependent termination of MEKK2 regulates temporal ERK activation required for proper hyperosmotic response

Takeshi Maruyama, Hisae Kadowaki, Noriaki Okamoto, Atsushi Nagai, Isao Naguro, Atsushi Matsuzawa, Hiroshi Shibuya, Keiji Tanaka, Shigeo Murata, Kohsuke Takeda, Hideki Nishitoh, Hidenori Ichijo

Research output: Contribution to journalArticle

32 Citations (Scopus)

Abstract

The extracellular signal-regulated kinase (ERK) pathway is an important signalling pathway that regulates a large number of cellular processes, including proliferation, differentiation and gene expression. Hyperosmotic stress activates the ERK pathway, whereas little is known about the regulatory mechanisms and physiological functions of ERK activation in hyperosmotic response. Here, we show that MAPK/ERK kinase kinase 2 (MEKK2), a member of the MAPKKK family, mediated the specific and transient activation of ERK, which was required for the induction of aquaporin 1 (AQP1) and AQP5 gene expression in response to hyperosmotic stress. Moreover, we identified the E3 ubiquitin ligase carboxyl terminus of Hsc70-interacting protein (CHIP) as a binding partner of MEKK2. Depletion of CHIP by small-interference RNA or gene targeting attenuated the degradation of MEKK2 and prolonged the ERK activity. Interestingly, hyperosmolality-induced gene expression of AQP1 and AQP5 was suppressed by CHIP depletion and was reversed by inhibition of the prolonged phase of ERK activity. These findings show that transient activation of the ERK pathway, which depends not only on MEKK2 activation, but also on CHIP-dependent MEKK2 degradation, is crucial for proper gene expression in hyperosmotic stress response.

Original languageEnglish
Pages (from-to)2501-2514
Number of pages14
JournalEMBO Journal
Volume29
Issue number15
DOIs
Publication statusPublished - 2010 Aug 4
Externally publishedYes

Fingerprint

HSC70 Heat-Shock Proteins
MAP Kinase Kinase Kinases
Extracellular Signal-Regulated MAP Kinases
Chemical activation
Gene expression
Aquaporin 1
Proteins
Gene Expression
Phosphotransferases
Ubiquitin-Protein Ligases
Gene Targeting
Mitogen-Activated Protein Kinase 1
Degradation
RNA Interference
Genes
RNA

Keywords

  • CHIP
  • hyperosmotic stress
  • MEKK2
  • ubiquitinproteasomal degradation

ASJC Scopus subject areas

  • Neuroscience(all)
  • Molecular Biology
  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)

Cite this

CHIP-dependent termination of MEKK2 regulates temporal ERK activation required for proper hyperosmotic response. / Maruyama, Takeshi; Kadowaki, Hisae; Okamoto, Noriaki; Nagai, Atsushi; Naguro, Isao; Matsuzawa, Atsushi; Shibuya, Hiroshi; Tanaka, Keiji; Murata, Shigeo; Takeda, Kohsuke; Nishitoh, Hideki; Ichijo, Hidenori.

In: EMBO Journal, Vol. 29, No. 15, 04.08.2010, p. 2501-2514.

Research output: Contribution to journalArticle

Maruyama, T, Kadowaki, H, Okamoto, N, Nagai, A, Naguro, I, Matsuzawa, A, Shibuya, H, Tanaka, K, Murata, S, Takeda, K, Nishitoh, H & Ichijo, H 2010, 'CHIP-dependent termination of MEKK2 regulates temporal ERK activation required for proper hyperosmotic response', EMBO Journal, vol. 29, no. 15, pp. 2501-2514. https://doi.org/10.1038/emboj.2010.141
Maruyama, Takeshi ; Kadowaki, Hisae ; Okamoto, Noriaki ; Nagai, Atsushi ; Naguro, Isao ; Matsuzawa, Atsushi ; Shibuya, Hiroshi ; Tanaka, Keiji ; Murata, Shigeo ; Takeda, Kohsuke ; Nishitoh, Hideki ; Ichijo, Hidenori. / CHIP-dependent termination of MEKK2 regulates temporal ERK activation required for proper hyperosmotic response. In: EMBO Journal. 2010 ; Vol. 29, No. 15. pp. 2501-2514.
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