CHO1, a mammalian kinesin-like protein, interacts with F-actin and is involved in the terminal phase of cytokinesis

Ryoko Kuriyama, Charles Gustus, Yasuhiko Terada, Yumi Uetake, Jurgita Matuliene

Research output: Contribution to journalArticle

106 Citations (Scopus)

Abstract

CHO1 is a kinesin-like protein of the mitotic kinesin-like protein (MKLP)1 subfamily present in central spindles and midbodies in mammalian cells. It is different from other subfamily members in that it contains an extra ∼300 bp in the COOH-terminal tail. Analysis of the chicken genomic sequence showed that heterogeneity is derived from alternative splicing, and exon 18 is expressed in only the CHO1 isoform. CHO1 and its truncated isoform MKLP1 are coexpressed in a single cell. Surprisingly, the sequence encoded by exon 18 possesses a capability to interact with F-actin, suggesting that CHO1 can associate with both microtubule and actin cytoskeletons. Microinjection of exon 18-specific antibodies did not result in any inhibitory effects on karyokinesis and early stages of cytokinesis. However, almost completely separated daughter cells became reunited to form a binulceate cell, suggesting that the exon 18 protein may not have a role in the formation and ingression of the contractile ring in the cortex. Rather, it might be involved directly or indirectly in the membrane events necessary for completion of the terminal phase of cytokinesis.

Original languageEnglish
Pages (from-to)783-790
Number of pages8
JournalJournal of Cell Biology
Volume156
Issue number5
DOIs
Publication statusPublished - 2002 Mar 4
Externally publishedYes

Fingerprint

Kinesin
Cytokinesis
Actins
Exons
Protein Isoforms
Proteins
Cell Nucleus Division
Alternative Splicing
Microinjections
Actin Cytoskeleton
Microtubules
Tail
Chickens
Membranes
Antibodies

Keywords

  • Actin
  • Alternative splicing
  • Cytokinesis
  • Kinesin-like protein
  • Midbody

ASJC Scopus subject areas

  • Cell Biology

Cite this

CHO1, a mammalian kinesin-like protein, interacts with F-actin and is involved in the terminal phase of cytokinesis. / Kuriyama, Ryoko; Gustus, Charles; Terada, Yasuhiko; Uetake, Yumi; Matuliene, Jurgita.

In: Journal of Cell Biology, Vol. 156, No. 5, 04.03.2002, p. 783-790.

Research output: Contribution to journalArticle

Kuriyama, Ryoko ; Gustus, Charles ; Terada, Yasuhiko ; Uetake, Yumi ; Matuliene, Jurgita. / CHO1, a mammalian kinesin-like protein, interacts with F-actin and is involved in the terminal phase of cytokinesis. In: Journal of Cell Biology. 2002 ; Vol. 156, No. 5. pp. 783-790.
@article{a18ed908624945cc979d88893e3a4865,
title = "CHO1, a mammalian kinesin-like protein, interacts with F-actin and is involved in the terminal phase of cytokinesis",
abstract = "CHO1 is a kinesin-like protein of the mitotic kinesin-like protein (MKLP)1 subfamily present in central spindles and midbodies in mammalian cells. It is different from other subfamily members in that it contains an extra ∼300 bp in the COOH-terminal tail. Analysis of the chicken genomic sequence showed that heterogeneity is derived from alternative splicing, and exon 18 is expressed in only the CHO1 isoform. CHO1 and its truncated isoform MKLP1 are coexpressed in a single cell. Surprisingly, the sequence encoded by exon 18 possesses a capability to interact with F-actin, suggesting that CHO1 can associate with both microtubule and actin cytoskeletons. Microinjection of exon 18-specific antibodies did not result in any inhibitory effects on karyokinesis and early stages of cytokinesis. However, almost completely separated daughter cells became reunited to form a binulceate cell, suggesting that the exon 18 protein may not have a role in the formation and ingression of the contractile ring in the cortex. Rather, it might be involved directly or indirectly in the membrane events necessary for completion of the terminal phase of cytokinesis.",
keywords = "Actin, Alternative splicing, Cytokinesis, Kinesin-like protein, Midbody",
author = "Ryoko Kuriyama and Charles Gustus and Yasuhiko Terada and Yumi Uetake and Jurgita Matuliene",
year = "2002",
month = "3",
day = "4",
doi = "10.1083/jcb.200109090",
language = "English",
volume = "156",
pages = "783--790",
journal = "Journal of Cell Biology",
issn = "0021-9525",
publisher = "Rockefeller University Press",
number = "5",

}

TY - JOUR

T1 - CHO1, a mammalian kinesin-like protein, interacts with F-actin and is involved in the terminal phase of cytokinesis

AU - Kuriyama, Ryoko

AU - Gustus, Charles

AU - Terada, Yasuhiko

AU - Uetake, Yumi

AU - Matuliene, Jurgita

PY - 2002/3/4

Y1 - 2002/3/4

N2 - CHO1 is a kinesin-like protein of the mitotic kinesin-like protein (MKLP)1 subfamily present in central spindles and midbodies in mammalian cells. It is different from other subfamily members in that it contains an extra ∼300 bp in the COOH-terminal tail. Analysis of the chicken genomic sequence showed that heterogeneity is derived from alternative splicing, and exon 18 is expressed in only the CHO1 isoform. CHO1 and its truncated isoform MKLP1 are coexpressed in a single cell. Surprisingly, the sequence encoded by exon 18 possesses a capability to interact with F-actin, suggesting that CHO1 can associate with both microtubule and actin cytoskeletons. Microinjection of exon 18-specific antibodies did not result in any inhibitory effects on karyokinesis and early stages of cytokinesis. However, almost completely separated daughter cells became reunited to form a binulceate cell, suggesting that the exon 18 protein may not have a role in the formation and ingression of the contractile ring in the cortex. Rather, it might be involved directly or indirectly in the membrane events necessary for completion of the terminal phase of cytokinesis.

AB - CHO1 is a kinesin-like protein of the mitotic kinesin-like protein (MKLP)1 subfamily present in central spindles and midbodies in mammalian cells. It is different from other subfamily members in that it contains an extra ∼300 bp in the COOH-terminal tail. Analysis of the chicken genomic sequence showed that heterogeneity is derived from alternative splicing, and exon 18 is expressed in only the CHO1 isoform. CHO1 and its truncated isoform MKLP1 are coexpressed in a single cell. Surprisingly, the sequence encoded by exon 18 possesses a capability to interact with F-actin, suggesting that CHO1 can associate with both microtubule and actin cytoskeletons. Microinjection of exon 18-specific antibodies did not result in any inhibitory effects on karyokinesis and early stages of cytokinesis. However, almost completely separated daughter cells became reunited to form a binulceate cell, suggesting that the exon 18 protein may not have a role in the formation and ingression of the contractile ring in the cortex. Rather, it might be involved directly or indirectly in the membrane events necessary for completion of the terminal phase of cytokinesis.

KW - Actin

KW - Alternative splicing

KW - Cytokinesis

KW - Kinesin-like protein

KW - Midbody

UR - http://www.scopus.com/inward/record.url?scp=0037018147&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0037018147&partnerID=8YFLogxK

U2 - 10.1083/jcb.200109090

DO - 10.1083/jcb.200109090

M3 - Article

VL - 156

SP - 783

EP - 790

JO - Journal of Cell Biology

JF - Journal of Cell Biology

SN - 0021-9525

IS - 5

ER -