Chromosome arm length, and a species-specific determinant, define chromosome arm width

Yasutaka Kakui*, Christopher Barrington, Yoshiharu Kusano, Rahul Thadani, Todd Fallesen, Toru Hirota, Frank Uhlmann

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract

Mitotic chromosomes in different organisms adopt various dimensions. What defines these dimensions is scarcely understood. Here, we compare mitotic chromosomes in budding and fission yeasts harboring similarly sized genomes distributed among 16 or 3 chromosomes, respectively. Hi-C analyses and superresolution microscopy reveal that budding yeast chromosomes are characterized by shorter-ranging mitotic chromatin contacts and are thinner compared with the thicker fission yeast chromosomes that contain longer-ranging mitotic contacts. These distinctions persist even after budding yeast chromosomes are fused to form three fission-yeast-length entities, revealing a species-specific organizing principle. Species-specific widths correlate with the known binding site intervals of the chromosomal condensin complex. Unexpectedly, within each species, we find that longer chromosome arms are always thicker and harbor longer-ranging contacts, a trend that we also observe with human chromosomes. Arm length as a chromosome width determinant informs mitotic chromosome formation models.

Original languageEnglish
Article number111753
JournalCell Reports
Volume41
Issue number10
DOIs
Publication statusPublished - 2022 Dec 6

Keywords

  • CP: Cell biology
  • H. sapiens
  • Hi-C
  • S. cerevisiae
  • S. pombe
  • chromatin interactions
  • chromosomes
  • condensin
  • mitosis
  • superresolution microscopy

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

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