Chromosome of the enterohemorrhagic Escherichia coli O157: H7; Comparative analysis with K-12 MG1655 revealed the acquisition of a large amount of foreign DNAs

Makoto Ohnishi, Chiho Tanaka, Satoru Kuhara, Kazuo Ishii, Masahira Hattori, Ken Kurokawa, Teruo Yasunaga, Kozo Makino, Hideo Shinagawa, Takahiro Murata, Keisuke Nakayama, Yoshiro Terawaki, Tetsuya Hayashi

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

A complete Xba I and Bln I cleavage map was constructed for the chromosome of an enterohemorrhagic Escherichia coli (EHEC) O157:H7 strain isolated from an outbreak in Sakai City, Japan, in 1996. A comparative chromosome analysis with E. coli K-12 strain MG1655 was made. The EHEC chromosome was approximately 5600 kb in length, 1 Mb larger than that of MG1655. Despite the marked difference in chromosome length, the location and direction of seven rRNA operons of the EHEC strain were similar to those for MG1655. Overall organization of genes common in both strains is also highly conserved. Chromosome expansion was observed throughout the EHEC chromosome, albeit in an uneven manner. A large portion of the chromosome enlargement was observed in the region surrounding the replication terminus, particularly in a segment containing the terA locus. Sample sequencing of 3627 random shotgun clones suggested the presence of approximately 1550 kb strain-specific DNAs on the EHEC chromosome, most of which are likely to be of foreign origin.

Original languageEnglish
Pages (from-to)361-368
Number of pages8
JournalDNA Research
Volume6
Issue number6
Publication statusPublished - 1999
Externally publishedYes

Fingerprint

Enterohemorrhagic Escherichia coli
Escherichia coli O157
Chromosomes
DNA
rRNA Operon
Firearms
Disease Outbreaks
Japan
Clone Cells
Escherichia coli

Keywords

  • Chromosome enlargement
  • Enterohemorrhagic E. coli O157:H7
  • Horizontal gene transfer
  • Physical map
  • rrn operon

ASJC Scopus subject areas

  • Genetics
  • Molecular Biology

Cite this

Chromosome of the enterohemorrhagic Escherichia coli O157 : H7; Comparative analysis with K-12 MG1655 revealed the acquisition of a large amount of foreign DNAs. / Ohnishi, Makoto; Tanaka, Chiho; Kuhara, Satoru; Ishii, Kazuo; Hattori, Masahira; Kurokawa, Ken; Yasunaga, Teruo; Makino, Kozo; Shinagawa, Hideo; Murata, Takahiro; Nakayama, Keisuke; Terawaki, Yoshiro; Hayashi, Tetsuya.

In: DNA Research, Vol. 6, No. 6, 1999, p. 361-368.

Research output: Contribution to journalArticle

Ohnishi, M, Tanaka, C, Kuhara, S, Ishii, K, Hattori, M, Kurokawa, K, Yasunaga, T, Makino, K, Shinagawa, H, Murata, T, Nakayama, K, Terawaki, Y & Hayashi, T 1999, 'Chromosome of the enterohemorrhagic Escherichia coli O157: H7; Comparative analysis with K-12 MG1655 revealed the acquisition of a large amount of foreign DNAs', DNA Research, vol. 6, no. 6, pp. 361-368.
Ohnishi, Makoto ; Tanaka, Chiho ; Kuhara, Satoru ; Ishii, Kazuo ; Hattori, Masahira ; Kurokawa, Ken ; Yasunaga, Teruo ; Makino, Kozo ; Shinagawa, Hideo ; Murata, Takahiro ; Nakayama, Keisuke ; Terawaki, Yoshiro ; Hayashi, Tetsuya. / Chromosome of the enterohemorrhagic Escherichia coli O157 : H7; Comparative analysis with K-12 MG1655 revealed the acquisition of a large amount of foreign DNAs. In: DNA Research. 1999 ; Vol. 6, No. 6. pp. 361-368.
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