Chromosome position at the spindle equator is regulated by chromokinesin and a bipolar microtubule array

Jun Takagi, Takeshi Itabashi, Kazuya Suzuki, Shin'Ichi Ishiwata

    Research output: Contribution to journalArticle

    4 Citations (Scopus)

    Abstract

    The chromosome alignment is mediated by polar ejection and poleward forces acting on the chromosome arm and kinetochores, respectively. Although components of the motile machinery such as chromokinesin have been characterized, their dynamics within the spindle is poorly understood. Here we show that a quantum dot (Qdot) binding up to four Xenopus chromokinesin (Xkid) molecules behaved like a nanosize chromosome arm in the meiotic spindle, which is self-organized in cytoplasmic egg extracts. Xkid-Qdots travelled long distances along microtubules by changing several tracks, resulting in their accumulation toward and distribution around the metaphase plate. The analysis indicated that the direction of motion and velocity depend on the distribution of microtubule polarity within the spindle. Thus, this mechanism is governed by chromokinesin motors, which is dependent on symmetrical microtubule orientation that may allow chromosomes to maintain their position around the spindle equator until correct microtubule-kinetochore attachment is established.

    Original languageEnglish
    Article number2808
    JournalScientific Reports
    Volume3
    DOIs
    Publication statusPublished - 2013

    Fingerprint

    Microtubules
    Chromosomes
    Kinetochores
    Quantum Dots
    Spindle Apparatus
    Metaphase
    Xenopus
    Ovum
    chromokinesin

    ASJC Scopus subject areas

    • General

    Cite this

    Chromosome position at the spindle equator is regulated by chromokinesin and a bipolar microtubule array. / Takagi, Jun; Itabashi, Takeshi; Suzuki, Kazuya; Ishiwata, Shin'Ichi.

    In: Scientific Reports, Vol. 3, 2808, 2013.

    Research output: Contribution to journalArticle

    Takagi, Jun ; Itabashi, Takeshi ; Suzuki, Kazuya ; Ishiwata, Shin'Ichi. / Chromosome position at the spindle equator is regulated by chromokinesin and a bipolar microtubule array. In: Scientific Reports. 2013 ; Vol. 3.
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