Chronological analysis of caloric restriction-induced alteration of fatty acid biosynthesis in white adipose tissue of rats

Naoyuki Okita, Takuro Tsuchiya, Mayumi Fukushima, Kaho Itakura, Keiko Yuguchi, Takumi Narita, Yukari Hashizume, Yuka Sudo, Takuya Chiba, Isao Shimokawa, Yoshikazu Higami

    Research output: Contribution to journalArticle

    6 Citations (Scopus)

    Abstract

    The beneficial actions of caloric restriction (CR) could be mediated in part by metabolic remodeling of white adipose tissue (WAT). Recently, we suggested that CR for 6. months increased the expressions of proteins involved in de novo fatty acid (FA) biosynthesis in WAT of 9-month-old rats. Herein, we compared the CR-induced chronological alterations of the expression of mRNAs and/or proteins involved in FA biosynthesis in the WAT and liver of rats subjected to CR starting from 3. months of age and their age-matched controls fed ad libitum. The findings suggested that CR was more effective on FA biosynthesis in WAT than in liver. In WAT, CR markedly increased the expressions of mRNAs and/or proteins involved in FA biosynthesis, including sterol regulatory element-binding protein 1c (SREBP1c), a master transcriptional regulator of FA biosynthesis, throughout the experimental period. Interestingly, the CR-enhanced upregulation was temporally attenuated at 5. months of age. CR markedly increased the nuclear phosphorylated form of Akt only at 3.5. months of age. In contrast, CR significantly reduced the expression of leptin at 9. months of age. The CR-induced upregulation was not observed in obese fa/fa Zucker rats homozygous for nonfunctional leptin receptor. Collectively, these data indicate that the V-shaped chronological alterations in WAT are regulated via SREBP1c, which is probably activated by CR duration-dependent modulation of both insulin and leptin signaling.

    Original languageEnglish
    Pages (from-to)59-66
    Number of pages8
    JournalExperimental Gerontology
    Volume63
    DOIs
    Publication statusPublished - 2015 Mar 1

    Fingerprint

    Caloric Restriction
    White Adipose Tissue
    Biosynthesis
    Rats
    Fatty Acids
    Tissue
    Sterol Regulatory Element Binding Protein 1
    Leptin
    Liver
    Up-Regulation
    Zucker Rats
    Leptin Receptors
    Messenger RNA
    Proteins
    Modulation
    Insulin

    Keywords

    • Adipose tissue
    • Caloric restriction
    • Fatty acid biosynthesis
    • SREBP1c

    ASJC Scopus subject areas

    • Ageing
    • Biochemistry
    • Cell Biology
    • Endocrinology
    • Genetics
    • Molecular Biology

    Cite this

    Chronological analysis of caloric restriction-induced alteration of fatty acid biosynthesis in white adipose tissue of rats. / Okita, Naoyuki; Tsuchiya, Takuro; Fukushima, Mayumi; Itakura, Kaho; Yuguchi, Keiko; Narita, Takumi; Hashizume, Yukari; Sudo, Yuka; Chiba, Takuya; Shimokawa, Isao; Higami, Yoshikazu.

    In: Experimental Gerontology, Vol. 63, 01.03.2015, p. 59-66.

    Research output: Contribution to journalArticle

    Okita, N, Tsuchiya, T, Fukushima, M, Itakura, K, Yuguchi, K, Narita, T, Hashizume, Y, Sudo, Y, Chiba, T, Shimokawa, I & Higami, Y 2015, 'Chronological analysis of caloric restriction-induced alteration of fatty acid biosynthesis in white adipose tissue of rats', Experimental Gerontology, vol. 63, pp. 59-66. https://doi.org/10.1016/j.exger.2015.01.043
    Okita, Naoyuki ; Tsuchiya, Takuro ; Fukushima, Mayumi ; Itakura, Kaho ; Yuguchi, Keiko ; Narita, Takumi ; Hashizume, Yukari ; Sudo, Yuka ; Chiba, Takuya ; Shimokawa, Isao ; Higami, Yoshikazu. / Chronological analysis of caloric restriction-induced alteration of fatty acid biosynthesis in white adipose tissue of rats. In: Experimental Gerontology. 2015 ; Vol. 63. pp. 59-66.
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