Circadian clock-dependent increase in salivary IgA secretion modulated by sympathetic receptor activation in mice

Misaki Wada, Kanami Orihara, Mayo Kamagata, Koki Hama, Hiroyuki Sasaki, Atsushi Haraguchi, Hiroki Miyakawa, Atsuhito Nakao, Shigenobu Shibata

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

The salivary gland is rhythmically controlled by sympathetic nerve activation from the suprachiasmatic nucleus (SCN), which functions as the main oscillator of circadian rhythms. In humans, salivary IgA concentrations reflect circadian rhythmicity, which peak during sleep. However, the mechanisms controlling this rhythmicity are not well understood. Therefore, we examined whether the timing of parasympathetic (pilocarpine) or sympathetic (norepinephrine; NE) activation affects IgA secretion in the saliva. The concentrations of saliva IgA modulated by pilocarpine activation or by a combination of pilocarpine and NE activation were the highest in the middle of the light period, independent of saliva flow rate. The circadian rhythm of IgA secretion was weakened by an SCN lesion and Clock gene mutation, suggesting the importance of the SCN and Clock gene on this rhythm. Adrenoceptor antagonists blocked both NE- and pilocarpine-induced basal secretion of IgA. Dimeric IgA binds to the polymeric immunoglobulin receptor (pIgR) on the basolateral surface of epithelial cells and forms the IgA-pIgR complex. The circadian rhythm of Pigr abundance peaked during the light period, suggesting pIgR expression upon rhythmic secretion of IgA. We speculate that activation of sympathetic nerves during sleep may protect from bacterial access to the epithelial surface through enhanced secretion of IgA.

Original languageEnglish
Article number8802
JournalScientific Reports
Volume7
Issue number1
DOIs
Publication statusPublished - 2017 Dec 1

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Circadian Clocks
Immunoglobulin A
Pilocarpine
Polymeric Immunoglobulin Receptors
Suprachiasmatic Nucleus
Circadian Rhythm
Saliva
Periodicity
Sleep
Light
Salivary Glands
Adrenergic Receptors
Genes
Norepinephrine
Epithelial Cells
Mutation

ASJC Scopus subject areas

  • General

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Circadian clock-dependent increase in salivary IgA secretion modulated by sympathetic receptor activation in mice. / Wada, Misaki; Orihara, Kanami; Kamagata, Mayo; Hama, Koki; Sasaki, Hiroyuki; Haraguchi, Atsushi; Miyakawa, Hiroki; Nakao, Atsuhito; Shibata, Shigenobu.

In: Scientific Reports, Vol. 7, No. 1, 8802, 01.12.2017.

Research output: Contribution to journalArticle

Wada, Misaki ; Orihara, Kanami ; Kamagata, Mayo ; Hama, Koki ; Sasaki, Hiroyuki ; Haraguchi, Atsushi ; Miyakawa, Hiroki ; Nakao, Atsuhito ; Shibata, Shigenobu. / Circadian clock-dependent increase in salivary IgA secretion modulated by sympathetic receptor activation in mice. In: Scientific Reports. 2017 ; Vol. 7, No. 1.
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