Circadian clock gene Period2 regulates a time-of-day-dependent variation in cutaneous anaphylactic reaction

Yuki Nakamura, Daisuke Harama, Naomi Shimokawa, Mutsuko Hara, Ryuyo Suzuki, Yu Tahara, Kayoko Ishimaru, Ryohei Katoh, Ko Okumura, Hideoki Ogawa, Shigenobu Shibata, Atsuhito Nakao

Research output: Contribution to journalArticle

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Abstract

Background: IgE-mediated immediate-type skin reaction shows a diurnal rhythm, although the precise mechanisms remain uncertain. Period2 (Per2) is a key circadian gene that is essential for endogenous clockworks in mammals. Objective: This study investigated whether Per2 regulates a time-of-day- dependent variation in IgE-mediated immediate-type skin reaction. Methods: The kinetics of a passive cutaneous anaphylactic reaction were compared between wild-type mice and mice with a loss-of-function mutation of Per2 (mPer2 m/m mice). The effects of adrenalectomy, aging, and dexamethasone on the kinetics of a passive cutaneous anaphylactic reaction were also examined. In addition, the extent of IgE-mediated degranulation in bone marrow-derived mast cells (BMMCs) was compared between wild-type and mPer2m/m mice. Results: A time-of-day-dependent variation in a passive cutaneous anaphylactic reaction observed in wild-type mice was absent in mPer2m/m mice and in adrenalectomized and aged mice associated with the loss of rhythmic secretion of corticosterone. In addition, mPer2m/m mice showed decreased sensitivity to the inhibitory effects of dexamethasone on the passive cutaneous anaphylactic reactions. IgE-mediated degranulation in BMMCs was comparable between wild-type and mPer2m/m mice, but Per2 mutation decreased sensitivity to the inhibitory effects of dexamethasone on IgE-mediated degranulation in BMMCs. Conclusion: A circadian oscillator, Per2, regulates a time-of-day-dependent variation in a passive cutaneous anaphylactic reaction in mice. Per2 may do so by controlling the rhythmic secretion of glucocorticoid from adrenal glands and/or by gating the glucocorticoid responses of mast cells to certain times of the day (possibly when Per2 levels are high in mast cells).

Original languageEnglish
JournalJournal of Allergy and Clinical Immunology
Volume127
Issue number4
DOIs
Publication statusPublished - 2011 Apr

Fingerprint

Circadian Clocks
Anaphylaxis
Skin
Mast Cells
Immunoglobulin E
Genes
Dexamethasone
Bone Marrow
Glucocorticoids
Mutation
Essential Genes
Adrenalectomy
Adrenal Glands
Corticosterone
Circadian Rhythm
Mammals

Keywords

  • anaphylactic reaction
  • Circadian rhythm
  • clock
  • IgE
  • mast cell
  • mouse
  • Period2

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

Cite this

Circadian clock gene Period2 regulates a time-of-day-dependent variation in cutaneous anaphylactic reaction. / Nakamura, Yuki; Harama, Daisuke; Shimokawa, Naomi; Hara, Mutsuko; Suzuki, Ryuyo; Tahara, Yu; Ishimaru, Kayoko; Katoh, Ryohei; Okumura, Ko; Ogawa, Hideoki; Shibata, Shigenobu; Nakao, Atsuhito.

In: Journal of Allergy and Clinical Immunology, Vol. 127, No. 4, 04.2011.

Research output: Contribution to journalArticle

Nakamura, Yuki ; Harama, Daisuke ; Shimokawa, Naomi ; Hara, Mutsuko ; Suzuki, Ryuyo ; Tahara, Yu ; Ishimaru, Kayoko ; Katoh, Ryohei ; Okumura, Ko ; Ogawa, Hideoki ; Shibata, Shigenobu ; Nakao, Atsuhito. / Circadian clock gene Period2 regulates a time-of-day-dependent variation in cutaneous anaphylactic reaction. In: Journal of Allergy and Clinical Immunology. 2011 ; Vol. 127, No. 4.
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abstract = "Background: IgE-mediated immediate-type skin reaction shows a diurnal rhythm, although the precise mechanisms remain uncertain. Period2 (Per2) is a key circadian gene that is essential for endogenous clockworks in mammals. Objective: This study investigated whether Per2 regulates a time-of-day- dependent variation in IgE-mediated immediate-type skin reaction. Methods: The kinetics of a passive cutaneous anaphylactic reaction were compared between wild-type mice and mice with a loss-of-function mutation of Per2 (mPer2 m/m mice). The effects of adrenalectomy, aging, and dexamethasone on the kinetics of a passive cutaneous anaphylactic reaction were also examined. In addition, the extent of IgE-mediated degranulation in bone marrow-derived mast cells (BMMCs) was compared between wild-type and mPer2m/m mice. Results: A time-of-day-dependent variation in a passive cutaneous anaphylactic reaction observed in wild-type mice was absent in mPer2m/m mice and in adrenalectomized and aged mice associated with the loss of rhythmic secretion of corticosterone. In addition, mPer2m/m mice showed decreased sensitivity to the inhibitory effects of dexamethasone on the passive cutaneous anaphylactic reactions. IgE-mediated degranulation in BMMCs was comparable between wild-type and mPer2m/m mice, but Per2 mutation decreased sensitivity to the inhibitory effects of dexamethasone on IgE-mediated degranulation in BMMCs. Conclusion: A circadian oscillator, Per2, regulates a time-of-day-dependent variation in a passive cutaneous anaphylactic reaction in mice. Per2 may do so by controlling the rhythmic secretion of glucocorticoid from adrenal glands and/or by gating the glucocorticoid responses of mast cells to certain times of the day (possibly when Per2 levels are high in mast cells).",
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AU - Nakamura, Yuki

AU - Harama, Daisuke

AU - Shimokawa, Naomi

AU - Hara, Mutsuko

AU - Suzuki, Ryuyo

AU - Tahara, Yu

AU - Ishimaru, Kayoko

AU - Katoh, Ryohei

AU - Okumura, Ko

AU - Ogawa, Hideoki

AU - Shibata, Shigenobu

AU - Nakao, Atsuhito

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N2 - Background: IgE-mediated immediate-type skin reaction shows a diurnal rhythm, although the precise mechanisms remain uncertain. Period2 (Per2) is a key circadian gene that is essential for endogenous clockworks in mammals. Objective: This study investigated whether Per2 regulates a time-of-day- dependent variation in IgE-mediated immediate-type skin reaction. Methods: The kinetics of a passive cutaneous anaphylactic reaction were compared between wild-type mice and mice with a loss-of-function mutation of Per2 (mPer2 m/m mice). The effects of adrenalectomy, aging, and dexamethasone on the kinetics of a passive cutaneous anaphylactic reaction were also examined. In addition, the extent of IgE-mediated degranulation in bone marrow-derived mast cells (BMMCs) was compared between wild-type and mPer2m/m mice. Results: A time-of-day-dependent variation in a passive cutaneous anaphylactic reaction observed in wild-type mice was absent in mPer2m/m mice and in adrenalectomized and aged mice associated with the loss of rhythmic secretion of corticosterone. In addition, mPer2m/m mice showed decreased sensitivity to the inhibitory effects of dexamethasone on the passive cutaneous anaphylactic reactions. IgE-mediated degranulation in BMMCs was comparable between wild-type and mPer2m/m mice, but Per2 mutation decreased sensitivity to the inhibitory effects of dexamethasone on IgE-mediated degranulation in BMMCs. Conclusion: A circadian oscillator, Per2, regulates a time-of-day-dependent variation in a passive cutaneous anaphylactic reaction in mice. Per2 may do so by controlling the rhythmic secretion of glucocorticoid from adrenal glands and/or by gating the glucocorticoid responses of mast cells to certain times of the day (possibly when Per2 levels are high in mast cells).

AB - Background: IgE-mediated immediate-type skin reaction shows a diurnal rhythm, although the precise mechanisms remain uncertain. Period2 (Per2) is a key circadian gene that is essential for endogenous clockworks in mammals. Objective: This study investigated whether Per2 regulates a time-of-day- dependent variation in IgE-mediated immediate-type skin reaction. Methods: The kinetics of a passive cutaneous anaphylactic reaction were compared between wild-type mice and mice with a loss-of-function mutation of Per2 (mPer2 m/m mice). The effects of adrenalectomy, aging, and dexamethasone on the kinetics of a passive cutaneous anaphylactic reaction were also examined. In addition, the extent of IgE-mediated degranulation in bone marrow-derived mast cells (BMMCs) was compared between wild-type and mPer2m/m mice. Results: A time-of-day-dependent variation in a passive cutaneous anaphylactic reaction observed in wild-type mice was absent in mPer2m/m mice and in adrenalectomized and aged mice associated with the loss of rhythmic secretion of corticosterone. In addition, mPer2m/m mice showed decreased sensitivity to the inhibitory effects of dexamethasone on the passive cutaneous anaphylactic reactions. IgE-mediated degranulation in BMMCs was comparable between wild-type and mPer2m/m mice, but Per2 mutation decreased sensitivity to the inhibitory effects of dexamethasone on IgE-mediated degranulation in BMMCs. Conclusion: A circadian oscillator, Per2, regulates a time-of-day-dependent variation in a passive cutaneous anaphylactic reaction in mice. Per2 may do so by controlling the rhythmic secretion of glucocorticoid from adrenal glands and/or by gating the glucocorticoid responses of mast cells to certain times of the day (possibly when Per2 levels are high in mast cells).

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KW - mast cell

KW - mouse

KW - Period2

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