Circadian clock gene Period2 regulates a time-of-day-dependent variation in cutaneous anaphylactic reaction

Yuki Nakamura, Daisuke Harama, Naomi Shimokawa, Mutsuko Hara, Ryuyo Suzuki, Yu Tahara, Kayoko Ishimaru, Ryohei Katoh, Ko Okumura, Hideoki Ogawa, Shigenobu Shibata, Atsuhito Nakao

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Abstract

Background: IgE-mediated immediate-type skin reaction shows a diurnal rhythm, although the precise mechanisms remain uncertain. Period2 (Per2) is a key circadian gene that is essential for endogenous clockworks in mammals. Objective: This study investigated whether Per2 regulates a time-of-day- dependent variation in IgE-mediated immediate-type skin reaction. Methods: The kinetics of a passive cutaneous anaphylactic reaction were compared between wild-type mice and mice with a loss-of-function mutation of Per2 (mPer2 m/m mice). The effects of adrenalectomy, aging, and dexamethasone on the kinetics of a passive cutaneous anaphylactic reaction were also examined. In addition, the extent of IgE-mediated degranulation in bone marrow-derived mast cells (BMMCs) was compared between wild-type and mPer2m/m mice. Results: A time-of-day-dependent variation in a passive cutaneous anaphylactic reaction observed in wild-type mice was absent in mPer2m/m mice and in adrenalectomized and aged mice associated with the loss of rhythmic secretion of corticosterone. In addition, mPer2m/m mice showed decreased sensitivity to the inhibitory effects of dexamethasone on the passive cutaneous anaphylactic reactions. IgE-mediated degranulation in BMMCs was comparable between wild-type and mPer2m/m mice, but Per2 mutation decreased sensitivity to the inhibitory effects of dexamethasone on IgE-mediated degranulation in BMMCs. Conclusion: A circadian oscillator, Per2, regulates a time-of-day-dependent variation in a passive cutaneous anaphylactic reaction in mice. Per2 may do so by controlling the rhythmic secretion of glucocorticoid from adrenal glands and/or by gating the glucocorticoid responses of mast cells to certain times of the day (possibly when Per2 levels are high in mast cells).

Original languageEnglish
Pages (from-to)1038-1045.e3
JournalJournal of Allergy and Clinical Immunology
Volume127
Issue number4
DOIs
Publication statusPublished - 2011 Apr

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Keywords

  • Circadian rhythm
  • IgE
  • Period2
  • anaphylactic reaction
  • clock
  • mast cell
  • mouse

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

Cite this

Nakamura, Y., Harama, D., Shimokawa, N., Hara, M., Suzuki, R., Tahara, Y., Ishimaru, K., Katoh, R., Okumura, K., Ogawa, H., Shibata, S., & Nakao, A. (2011). Circadian clock gene Period2 regulates a time-of-day-dependent variation in cutaneous anaphylactic reaction. Journal of Allergy and Clinical Immunology, 127(4), 1038-1045.e3. https://doi.org/10.1016/j.jaci.2011.02.006