Circadian Clock mutation in dams disrupts nursing behavior and growth of pups

Kyoko Hoshino, Yukako Wakatsuki, Masayuki Iigo, Shigenobu Shibata

    Research output: Contribution to journalArticle

    37 Citations (Scopus)

    Abstract

    To understand the role of the circadian molecular clock in mouse reproduction, we investigated the daily rhythms associated with nursing and pup growth in Clock-mutant mice maintained under light-dark housing conditions. The daily rhythm associated with the maternal behavior of crouching had a strong diurnal peak and two weak nocturnal peaks in wild-type dams, whereas homozygotes (Clock/Clock) exhibited no significant peaks in activity. Wild-type, but not Clock-mutant, dams showed high, rhythmic levels of prolactin content in serum that corresponded with crouching. Pup body weight increased at a significantly slower rate in Clock-mutant dams compared with wild-type dams under all experimental conditions when the pups ranged from 10-15 in number. Heterozygote dams equally bred wild-type, heterozygote, or homozygote pups. The amount of milk secreted from dams, as calculated by the increase in pup body weight through suckling, was lower in Clock-mutant mothers vs. wild-type mice. When Clock-mutant dams gave birth to more than 10 pups, survival was poor for offspring until the time of weaning. The present results demonstrate that Clock mutation disrupts daily maternal behavior and the growth and survival rate of pups, especially with the breeding of more than 10 pups.

    Original languageEnglish
    Pages (from-to)1916-1923
    Number of pages8
    JournalEndocrinology
    Volume147
    Issue number4
    DOIs
    Publication statusPublished - 2006 Apr

    Fingerprint

    Circadian Clocks
    Maternal Behavior
    Nursing
    Homozygote
    Heterozygote
    Mutation
    Growth
    Body Weight
    Weaning
    Prolactin
    Breeding
    Reproduction
    Milk
    Survival Rate
    Mothers
    Parturition
    Light
    Serum

    ASJC Scopus subject areas

    • Endocrinology
    • Endocrinology, Diabetes and Metabolism

    Cite this

    Circadian Clock mutation in dams disrupts nursing behavior and growth of pups. / Hoshino, Kyoko; Wakatsuki, Yukako; Iigo, Masayuki; Shibata, Shigenobu.

    In: Endocrinology, Vol. 147, No. 4, 04.2006, p. 1916-1923.

    Research output: Contribution to journalArticle

    Hoshino, Kyoko ; Wakatsuki, Yukako ; Iigo, Masayuki ; Shibata, Shigenobu. / Circadian Clock mutation in dams disrupts nursing behavior and growth of pups. In: Endocrinology. 2006 ; Vol. 147, No. 4. pp. 1916-1923.
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