Circadian profile of Per gene mRNA expression in the suprachiasmatic nucleus, paraventricular nucleus, and pineal body of aged rats

Makoto Asai, Yuko Yoshinobu, Satoshi Kaneko, Akiko Mori, Takato Nikaido, Takahiro Moriya, Masashi Akiyama, Shigenobu Shibata

    Research output: Contribution to journalArticle

    106 Citations (Scopus)

    Abstract

    Aging alters circadian components such as the free-running period, the day-to-night activity ratio and photic entrainment in behavioral rhythms, and 2-deoxyglucose uptakes and neuronal firing in the suprachiasmatic nucleus (SCN). A core clock mechanism in the mouse SCN appears to involve a transcriptional feedback loop in which Period (Per) and Cryptochrome (Cry) genes play a role in negative feedback. The circadian rhythm systems include photic entrainment, clock oscillation, and outputs of clock information such as melatonin production. In this experiment, we examined clock gene expression to determine whether circadian input, oscillation, and output are disrupted with aging. Circadian expression profiles of rPer1, rPer2, or rCry1 mRNA were very similar in the SCN, the paraventricular nucleus of the hypothalamus (PVN), and the pineal body of young and aged (22-26 months) rats. On the other hand, the photic stimulation-induced rapid expression of Per1 and Per2 in the SCN was reduced with aging. The present results suggest that the molecular mechanism of clock oscillation in the SCN, PVN, and pineal body is preserved against aging, whereas the impairment of Per1 induction in the SCN after light stimulation may result in impaired behavioral photic entrainment in aged rats.

    Original languageEnglish
    Pages (from-to)1133-1139
    Number of pages7
    JournalJournal of Neuroscience Research
    Volume66
    Issue number6
    DOIs
    Publication statusPublished - 2001 Dec 15

    Fingerprint

    Suprachiasmatic Nucleus
    Pineal Gland
    Paraventricular Hypothalamic Nucleus
    Gene Expression
    Messenger RNA
    Hypothalamus
    Cryptochromes
    Photic Stimulation
    Deoxyglucose
    Melatonin
    Circadian Rhythm
    Light
    Genes

    Keywords

    • Aging
    • Circadian rhythm
    • Clock gene
    • Pineal body
    • Suprachiasmatic nucleus

    ASJC Scopus subject areas

    • Neuroscience(all)

    Cite this

    Circadian profile of Per gene mRNA expression in the suprachiasmatic nucleus, paraventricular nucleus, and pineal body of aged rats. / Asai, Makoto; Yoshinobu, Yuko; Kaneko, Satoshi; Mori, Akiko; Nikaido, Takato; Moriya, Takahiro; Akiyama, Masashi; Shibata, Shigenobu.

    In: Journal of Neuroscience Research, Vol. 66, No. 6, 15.12.2001, p. 1133-1139.

    Research output: Contribution to journalArticle

    Asai, Makoto ; Yoshinobu, Yuko ; Kaneko, Satoshi ; Mori, Akiko ; Nikaido, Takato ; Moriya, Takahiro ; Akiyama, Masashi ; Shibata, Shigenobu. / Circadian profile of Per gene mRNA expression in the suprachiasmatic nucleus, paraventricular nucleus, and pineal body of aged rats. In: Journal of Neuroscience Research. 2001 ; Vol. 66, No. 6. pp. 1133-1139.
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    abstract = "Aging alters circadian components such as the free-running period, the day-to-night activity ratio and photic entrainment in behavioral rhythms, and 2-deoxyglucose uptakes and neuronal firing in the suprachiasmatic nucleus (SCN). A core clock mechanism in the mouse SCN appears to involve a transcriptional feedback loop in which Period (Per) and Cryptochrome (Cry) genes play a role in negative feedback. The circadian rhythm systems include photic entrainment, clock oscillation, and outputs of clock information such as melatonin production. In this experiment, we examined clock gene expression to determine whether circadian input, oscillation, and output are disrupted with aging. Circadian expression profiles of rPer1, rPer2, or rCry1 mRNA were very similar in the SCN, the paraventricular nucleus of the hypothalamus (PVN), and the pineal body of young and aged (22-26 months) rats. On the other hand, the photic stimulation-induced rapid expression of Per1 and Per2 in the SCN was reduced with aging. The present results suggest that the molecular mechanism of clock oscillation in the SCN, PVN, and pineal body is preserved against aging, whereas the impairment of Per1 induction in the SCN after light stimulation may result in impaired behavioral photic entrainment in aged rats.",
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