Circadian regulation of allergic reactions by the mast cell clock in mice

Yuki Nakamura, Nobuhiro Nakano, Kayoko Ishimaru, Mutsuko Hara, Takako Ikegami, Yu Tahara, Ryohei Katoh, Hideoki Ogawa, Ko Okumura, Shigenobu Shibata, Chiharu Nishiyama, Atsuhito Nakao

Research output: Contribution to journalArticle

38 Citations (Scopus)

Abstract

Background It remains elusive how allergic symptoms exhibit prominent 24-hour variations. In mammals the circadian clocks present in nearly all cells, including mast cells, drive the daily rhythms of physiology. Recently, we have shown that the circadian clocks drive the daily rhythms in IgE/mast cell-mediated allergic reactions. However, the precise mechanisms, particularly the specific roles of the mast cell-intrinsic clockwork in temporal regulation, remain unclear. Objective We determined whether the mast cell clockwork contributes to the temporal regulation of IgE/mast cell-mediated allergic reaction. Methods The kinetics of a time of day-dependent variation in passive cutaneous anaphylactic reactions were compared between mast cell-deficient mice reconstituted with bone marrow-derived cultured mast cells generated from mice with a wild-type allele and a dominant negative type mutation of the key clock gene Clock. We also examined the temporal responses of wild-type and Clock-mutated bone marrow-derived cultured mast cells to IgE stimulation in vitro. Furthermore, factors influencing the mast cell clockwork were determined by using in vivo imaging. Results The Clock mutation in mast cells resulted in the absence of temporal variations in IgE-mediated degranulation in mast cells both in vivo and in vitro associated with the loss of temporal regulation of FcεRI expression and signaling. Additionally, adrenalectomy abolished the mast cell clockwork in vivo. Conclusion The mast cell-intrinsic clockwork, entrained by humoral factors from the adrenal gland, primarily contributes to the temporal regulation of IgE/mast cell-mediated allergic reactions. Our results reveal a novel regulatory mechanism for IgE-mediated mast cell responses that might underlie the circadian pathophysiology in patients with allergic diseases.

Original languageEnglish
JournalJournal of Allergy and Clinical Immunology
Volume133
Issue number2
DOIs
Publication statusPublished - 2014 Feb

Fingerprint

Mast Cells
Hypersensitivity
Immunoglobulin E
Circadian Clocks
Cultured Cells
Bone Marrow
Mutation
Adrenalectomy
Anaphylaxis
Adrenal Glands
Mammals
Alleles

Keywords

  • allergy
  • IgE
  • mast cells
  • mouse
  • The circadian clock

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

Cite this

Circadian regulation of allergic reactions by the mast cell clock in mice. / Nakamura, Yuki; Nakano, Nobuhiro; Ishimaru, Kayoko; Hara, Mutsuko; Ikegami, Takako; Tahara, Yu; Katoh, Ryohei; Ogawa, Hideoki; Okumura, Ko; Shibata, Shigenobu; Nishiyama, Chiharu; Nakao, Atsuhito.

In: Journal of Allergy and Clinical Immunology, Vol. 133, No. 2, 02.2014.

Research output: Contribution to journalArticle

Nakamura, Y, Nakano, N, Ishimaru, K, Hara, M, Ikegami, T, Tahara, Y, Katoh, R, Ogawa, H, Okumura, K, Shibata, S, Nishiyama, C & Nakao, A 2014, 'Circadian regulation of allergic reactions by the mast cell clock in mice', Journal of Allergy and Clinical Immunology, vol. 133, no. 2. https://doi.org/10.1016/j.jaci.2013.07.040
Nakamura, Yuki ; Nakano, Nobuhiro ; Ishimaru, Kayoko ; Hara, Mutsuko ; Ikegami, Takako ; Tahara, Yu ; Katoh, Ryohei ; Ogawa, Hideoki ; Okumura, Ko ; Shibata, Shigenobu ; Nishiyama, Chiharu ; Nakao, Atsuhito. / Circadian regulation of allergic reactions by the mast cell clock in mice. In: Journal of Allergy and Clinical Immunology. 2014 ; Vol. 133, No. 2.
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