Circadian rhythms in the synthesis and degradation of a master clock protein KaiC in cyanobacteria

Keiko Imai, Taeko Nishiwaki, Takao Kondo, Hideo Iwasaki

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

A circadian rhythm in the accumulation of the core clock protein KaiC has been proposed to be important for proper circadian timing in the cyanobacterium Synechococcus elongatus PCC 7942 under continuous light conditions. Cycling in the abundance of the KaiC protein is delayed to the rhythm of its mRNA by ∼8 h, consistent with the proposed function of KaiC as a negative feedback regulator of kaiBC transcription. Here, we present temporal profiles of the synthesis and degradation of KaiC protein that determine the rhythm of its accumulation. The rate of KaiC synthesis shows a robust circadian oscillation, which is delayed to the mRNA rhythm slightly and advances the rhythm of KaiC accumulation by ∼6 h. The stability of KaiC protein also shows circadian fluctuations, such that KaiC degradation is suppressed during the mid-subjective night. These results suggest that transcriptional, translational, and posttranslational processes are important for the proper circadian changes in KaiC accumulation. Moreover, the turnovers of the phosphorylated and non-phosphorylated forms of KaiC show robust circadian rhythms with an anti-phase relationship to each other. Interestingly, when translation was inhibited, KaiC degradation and phosphorylation proceeded within at least 4 h in a circadian phase-dependent manner. Thus, the circadian timing seems flexible even when any perturbation in protein synthesis occurs.

Original languageEnglish
Pages (from-to)36534-36539
Number of pages6
JournalJournal of Biological Chemistry
Volume279
Issue number35
DOIs
Publication statusPublished - 2004 Aug 27
Externally publishedYes

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Circadian Rhythm
Clocks
Degradation
Synechococcus
Messenger RNA
Proteins
Protein Stability
Cyanobacteria
Proteolysis
Phosphorylation
Light
Transcription
Cyanobacteria KaiC protein
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ASJC Scopus subject areas

  • Biochemistry

Cite this

Circadian rhythms in the synthesis and degradation of a master clock protein KaiC in cyanobacteria. / Imai, Keiko; Nishiwaki, Taeko; Kondo, Takao; Iwasaki, Hideo.

In: Journal of Biological Chemistry, Vol. 279, No. 35, 27.08.2004, p. 36534-36539.

Research output: Contribution to journalArticle

Imai, Keiko ; Nishiwaki, Taeko ; Kondo, Takao ; Iwasaki, Hideo. / Circadian rhythms in the synthesis and degradation of a master clock protein KaiC in cyanobacteria. In: Journal of Biological Chemistry. 2004 ; Vol. 279, No. 35. pp. 36534-36539.
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