Circularization of linear viroid RNA via 2′-phosphomonoester, 3′, 5′-phosphodiester bonds by a novel type of RNA ligase from wheat germ and Chlamydomonas

Yo Kikuchi, Kazimierz Tyc, Witold Filipowicz, Heinz L. Sänger, Hans J. Gross

Research output: Contribution to journalArticle

51 Citations (Scopus)

Abstract

A novel type of RNA ligase activity in extracts of wheat germ or Chlamydomonas requires 2′,3′-cyclic phosphate and 5′-phosphate ends for ligation to form a 2′-phosphomonoester, 3′,5′-phosphodiester bond. Using 5′-32P-labeled linear PSTV, we demonstrate that RNase T1-nicked viroid predominantly forms -G3′-P2′-P-5′U-bonds. Natural linear PSTV, however, forms main--G3′-P2′-P-5′A-bonds upon enzymatic circularization. We show that natural linear PSTV RNA has nicks between C181 and A182, or between C348 and A349, and that consequently C181 and C348 carry 2′,3′-cyclophosphate termini.

Original languageEnglish
Pages (from-to)7521-7529
Number of pages9
JournalNucleic Acids Research
Volume10
Issue number23
DOIs
Publication statusPublished - 1982 Dec 11
Externally publishedYes

Fingerprint

Viroids
Chlamydomonas
Wheat
Ligases
RNA
Triticum
Phosphates
Ribonuclease T1
Phosphate
Ligation
Demonstrate

ASJC Scopus subject areas

  • Statistics, Probability and Uncertainty
  • Applied Mathematics
  • Health, Toxicology and Mutagenesis
  • Toxicology
  • Genetics(clinical)
  • Genetics

Cite this

Circularization of linear viroid RNA via 2′-phosphomonoester, 3′, 5′-phosphodiester bonds by a novel type of RNA ligase from wheat germ and Chlamydomonas. / Kikuchi, Yo; Tyc, Kazimierz; Filipowicz, Witold; Sänger, Heinz L.; Gross, Hans J.

In: Nucleic Acids Research, Vol. 10, No. 23, 11.12.1982, p. 7521-7529.

Research output: Contribution to journalArticle

Kikuchi, Yo ; Tyc, Kazimierz ; Filipowicz, Witold ; Sänger, Heinz L. ; Gross, Hans J. / Circularization of linear viroid RNA via 2′-phosphomonoester, 3′, 5′-phosphodiester bonds by a novel type of RNA ligase from wheat germ and Chlamydomonas. In: Nucleic Acids Research. 1982 ; Vol. 10, No. 23. pp. 7521-7529.
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