Circulatory half-life time of artificial red blood cell (hemoglobin-vesicles) and their distribution to metabolic organs

Keitaro Sou, Beth Goins, William T. Phillips, Hiromi Sakai, Shinji Takeoka, Eishun Tsuchida

    Research output: Chapter in Book/Report/Conference proceedingConference contribution

    Abstract

    Phospholipid vesicles encapsulating concentrated human hemoglobin (Hb-vesicles: HbV) have a membrane structure similar to that of red blood cells (RBC). We report the pharmacokinetics of HbV and empty vesicles (EV) that do not contain Hb, in rats and rabbits to evaluate the potential of HbV as a RBC substitute. The samples were labeled with technetium-99m (99mTc), and then intravenously infused into animals at 14 mL/kg to measure the kinetics of HbV elimination from blood and distribution to the organs. The circulation half-life times were 34.8 and 62.6 h for HbV, and 29.3 and 57.3 h for EV in rats and rabbits, respectively. At 48 h after infusion, the liver and spleen of both rats and rabbits had significant concentrations of HbV and EV. These organs would be reasonable to metabolize HbV because they have mononuclear phagocyte systems to introduce the red blood cells into metabolic process.

    Original languageEnglish
    Title of host publicationPolymer Preprints, Japan
    Pages2271
    Number of pages1
    Volume54
    Edition1
    Publication statusPublished - 2005
    Event54th SPSJ Annual Meeting 2005 - Yokohama
    Duration: 2005 May 252005 May 27

    Other

    Other54th SPSJ Annual Meeting 2005
    CityYokohama
    Period05/5/2505/5/27

    Fingerprint

    Hemoglobin
    Blood
    Cells
    Rats
    Technetium
    Membrane structures
    Pharmacokinetics
    Phospholipids
    Liver
    Animals
    Kinetics

    Keywords

    • Circulatory half-life time
    • Hemoglobin
    • Organ distribution
    • Pharmacokinetics
    • Phospholipid vesicles
    • Red blood cell substitute

    ASJC Scopus subject areas

    • Engineering(all)

    Cite this

    Sou, K., Goins, B., Phillips, W. T., Sakai, H., Takeoka, S., & Tsuchida, E. (2005). Circulatory half-life time of artificial red blood cell (hemoglobin-vesicles) and their distribution to metabolic organs. In Polymer Preprints, Japan (1 ed., Vol. 54, pp. 2271)

    Circulatory half-life time of artificial red blood cell (hemoglobin-vesicles) and their distribution to metabolic organs. / Sou, Keitaro; Goins, Beth; Phillips, William T.; Sakai, Hiromi; Takeoka, Shinji; Tsuchida, Eishun.

    Polymer Preprints, Japan. Vol. 54 1. ed. 2005. p. 2271.

    Research output: Chapter in Book/Report/Conference proceedingConference contribution

    Sou, K, Goins, B, Phillips, WT, Sakai, H, Takeoka, S & Tsuchida, E 2005, Circulatory half-life time of artificial red blood cell (hemoglobin-vesicles) and their distribution to metabolic organs. in Polymer Preprints, Japan. 1 edn, vol. 54, pp. 2271, 54th SPSJ Annual Meeting 2005, Yokohama, 05/5/25.
    Sou K, Goins B, Phillips WT, Sakai H, Takeoka S, Tsuchida E. Circulatory half-life time of artificial red blood cell (hemoglobin-vesicles) and their distribution to metabolic organs. In Polymer Preprints, Japan. 1 ed. Vol. 54. 2005. p. 2271
    Sou, Keitaro ; Goins, Beth ; Phillips, William T. ; Sakai, Hiromi ; Takeoka, Shinji ; Tsuchida, Eishun. / Circulatory half-life time of artificial red blood cell (hemoglobin-vesicles) and their distribution to metabolic organs. Polymer Preprints, Japan. Vol. 54 1. ed. 2005. pp. 2271
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    abstract = "Phospholipid vesicles encapsulating concentrated human hemoglobin (Hb-vesicles: HbV) have a membrane structure similar to that of red blood cells (RBC). We report the pharmacokinetics of HbV and empty vesicles (EV) that do not contain Hb, in rats and rabbits to evaluate the potential of HbV as a RBC substitute. The samples were labeled with technetium-99m (99mTc), and then intravenously infused into animals at 14 mL/kg to measure the kinetics of HbV elimination from blood and distribution to the organs. The circulation half-life times were 34.8 and 62.6 h for HbV, and 29.3 and 57.3 h for EV in rats and rabbits, respectively. At 48 h after infusion, the liver and spleen of both rats and rabbits had significant concentrations of HbV and EV. These organs would be reasonable to metabolize HbV because they have mononuclear phagocyte systems to introduce the red blood cells into metabolic process.",
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    AU - Takeoka, Shinji

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