Circulatory half-life time of artificial red blood cell (hemoglobin-vesicles) and their distribution to metabolic organs

Keitaro Sou; Beth Goins; William T. Phillips; Hiromi Sakai; Shinji Takeoka; Eishun Tsuchida

Circulatory half-life time of artificial red blood cell (hemoglobin-vesicles) and their distribution to metabolic organs

Keitaro Sou^*, Beth Goins, William T. Phillips, Hiromi Sakai, Shinji Takeoka, Eishun Tsuchida

^*Corresponding author for this work

School of Advanced Science and Engineering

Research output: Contribution to conference › Paper › peer-review

Abstract

Phospholipid vesicles encapsulating concentrated human hemoglobin (Hb-vesicles: HbV) have a membrane structure similar to that of red blood cells (RBC). We report the pharmacokinetics of HbV and empty vesicles (EV) that do not contain Hb, in rats and rabbits to evaluate the potential of HbV as a RBC substitute. The samples were labeled with technetium-99m (^99mTc), and then intravenously infused into animals at 14 mL/kg to measure the kinetics of HbV elimination from blood and distribution to the organs. The circulation half-life times were 34.8 and 62.6 h for HbV, and 29.3 and 57.3 h for EV in rats and rabbits, respectively. At 48 h after infusion, the liver and spleen of both rats and rabbits had significant concentrations of HbV and EV. These organs would be reasonable to metabolize HbV because they have mononuclear phagocyte systems to introduce the red blood cells into metabolic process.

Original language	English
Number of pages	1
Publication status	Published - 2005 Dec 1
Event	54th SPSJ Annual Meeting 2005 - Yokohama, Japan Duration: 2005 May 25 → 2005 May 27

Conference

Conference	54th SPSJ Annual Meeting 2005
Country/Territory	Japan
City	Yokohama
Period	05/5/25 → 05/5/27

Keywords

Circulatory half-life time
Hemoglobin
Organ distribution
Pharmacokinetics
Phospholipid vesicles
Red blood cell substitute

ASJC Scopus subject areas

Engineering(all)

Cite this

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title = "Circulatory half-life time of artificial red blood cell (hemoglobin-vesicles) and their distribution to metabolic organs",

abstract = "Phospholipid vesicles encapsulating concentrated human hemoglobin (Hb-vesicles: HbV) have a membrane structure similar to that of red blood cells (RBC). We report the pharmacokinetics of HbV and empty vesicles (EV) that do not contain Hb, in rats and rabbits to evaluate the potential of HbV as a RBC substitute. The samples were labeled with technetium-99m (99mTc), and then intravenously infused into animals at 14 mL/kg to measure the kinetics of HbV elimination from blood and distribution to the organs. The circulation half-life times were 34.8 and 62.6 h for HbV, and 29.3 and 57.3 h for EV in rats and rabbits, respectively. At 48 h after infusion, the liver and spleen of both rats and rabbits had significant concentrations of HbV and EV. These organs would be reasonable to metabolize HbV because they have mononuclear phagocyte systems to introduce the red blood cells into metabolic process.",

keywords = "Circulatory half-life time, Hemoglobin, Organ distribution, Pharmacokinetics, Phospholipid vesicles, Red blood cell substitute",

author = "Keitaro Sou and Beth Goins and Phillips, {William T.} and Hiromi Sakai and Shinji Takeoka and Eishun Tsuchida",

year = "2005",

month = dec,

day = "1",

language = "English",

note = "54th SPSJ Annual Meeting 2005 ; Conference date: 25-05-2005 Through 27-05-2005",

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T1 - Circulatory half-life time of artificial red blood cell (hemoglobin-vesicles) and their distribution to metabolic organs

AU - Sou, Keitaro

AU - Goins, Beth

AU - Phillips, William T.

AU - Sakai, Hiromi

AU - Takeoka, Shinji

AU - Tsuchida, Eishun

PY - 2005/12/1

Y1 - 2005/12/1

N2 - Phospholipid vesicles encapsulating concentrated human hemoglobin (Hb-vesicles: HbV) have a membrane structure similar to that of red blood cells (RBC). We report the pharmacokinetics of HbV and empty vesicles (EV) that do not contain Hb, in rats and rabbits to evaluate the potential of HbV as a RBC substitute. The samples were labeled with technetium-99m (99mTc), and then intravenously infused into animals at 14 mL/kg to measure the kinetics of HbV elimination from blood and distribution to the organs. The circulation half-life times were 34.8 and 62.6 h for HbV, and 29.3 and 57.3 h for EV in rats and rabbits, respectively. At 48 h after infusion, the liver and spleen of both rats and rabbits had significant concentrations of HbV and EV. These organs would be reasonable to metabolize HbV because they have mononuclear phagocyte systems to introduce the red blood cells into metabolic process.

AB - Phospholipid vesicles encapsulating concentrated human hemoglobin (Hb-vesicles: HbV) have a membrane structure similar to that of red blood cells (RBC). We report the pharmacokinetics of HbV and empty vesicles (EV) that do not contain Hb, in rats and rabbits to evaluate the potential of HbV as a RBC substitute. The samples were labeled with technetium-99m (99mTc), and then intravenously infused into animals at 14 mL/kg to measure the kinetics of HbV elimination from blood and distribution to the organs. The circulation half-life times were 34.8 and 62.6 h for HbV, and 29.3 and 57.3 h for EV in rats and rabbits, respectively. At 48 h after infusion, the liver and spleen of both rats and rabbits had significant concentrations of HbV and EV. These organs would be reasonable to metabolize HbV because they have mononuclear phagocyte systems to introduce the red blood cells into metabolic process.

KW - Circulatory half-life time

KW - Hemoglobin

KW - Organ distribution

KW - Pharmacokinetics

KW - Phospholipid vesicles

KW - Red blood cell substitute

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M3 - Paper

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T2 - 54th SPSJ Annual Meeting 2005

Y2 - 25 May 2005 through 27 May 2005

ER -

Circulatory half-life time of artificial red blood cell (hemoglobin-vesicles) and their distribution to metabolic organs

Abstract

Conference

Keywords

ASJC Scopus subject areas

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