Cis and trans reactions of hairpin ribozymes derived from the negative strand of arabis mosaic virus satellite RNA.

R. Tomita, Y. Morikawa, S. Hisamatsu, K. Suzuki-Fujita, T. Tanaka, S. Sonoki, Yo Kikuchi

Research output: Contribution to journalArticle

Abstract

We have previously shown that the hairpin ribozyme-like structure of the negative strand of the satellite RNA of arabis mosaic virus [(- )sArMV] has indeed ribozyme activity. However, some mutagenesis analyses revealed surprisingly that the wild type ribozyme was less active than almost all the other mutant ribozymes tested. These results were derived from a trans-acting system. Here we tested this ribozyme activity in a cis-acting system. We show that the (-)s ArMV hairpin ribozyme has different target-site specificities between cis and trans cleavages. The wild type ribozyme has the highest self-cleaving activity among the ribozyme variants tested.

Original languageEnglish
Pages (from-to)117-118
Number of pages2
JournalNucleic acids symposium series
Issue number34
Publication statusPublished - 1995
Externally publishedYes

Fingerprint

Arabis
Satellite RNAs
Mosaic Viruses
Catalytic RNA
Mutagenesis
hairpin ribozyme

Cite this

Tomita, R., Morikawa, Y., Hisamatsu, S., Suzuki-Fujita, K., Tanaka, T., Sonoki, S., & Kikuchi, Y. (1995). Cis and trans reactions of hairpin ribozymes derived from the negative strand of arabis mosaic virus satellite RNA. Nucleic acids symposium series, (34), 117-118.

Cis and trans reactions of hairpin ribozymes derived from the negative strand of arabis mosaic virus satellite RNA. / Tomita, R.; Morikawa, Y.; Hisamatsu, S.; Suzuki-Fujita, K.; Tanaka, T.; Sonoki, S.; Kikuchi, Yo.

In: Nucleic acids symposium series, No. 34, 1995, p. 117-118.

Research output: Contribution to journalArticle

Tomita, R, Morikawa, Y, Hisamatsu, S, Suzuki-Fujita, K, Tanaka, T, Sonoki, S & Kikuchi, Y 1995, 'Cis and trans reactions of hairpin ribozymes derived from the negative strand of arabis mosaic virus satellite RNA.', Nucleic acids symposium series, no. 34, pp. 117-118.
Tomita R, Morikawa Y, Hisamatsu S, Suzuki-Fujita K, Tanaka T, Sonoki S et al. Cis and trans reactions of hairpin ribozymes derived from the negative strand of arabis mosaic virus satellite RNA. Nucleic acids symposium series. 1995;(34):117-118.
Tomita, R. ; Morikawa, Y. ; Hisamatsu, S. ; Suzuki-Fujita, K. ; Tanaka, T. ; Sonoki, S. ; Kikuchi, Yo. / Cis and trans reactions of hairpin ribozymes derived from the negative strand of arabis mosaic virus satellite RNA. In: Nucleic acids symposium series. 1995 ; No. 34. pp. 117-118.
@article{2f6fd439125f4ce6a16f998cdc248133,
title = "Cis and trans reactions of hairpin ribozymes derived from the negative strand of arabis mosaic virus satellite RNA.",
abstract = "We have previously shown that the hairpin ribozyme-like structure of the negative strand of the satellite RNA of arabis mosaic virus [(- )sArMV] has indeed ribozyme activity. However, some mutagenesis analyses revealed surprisingly that the wild type ribozyme was less active than almost all the other mutant ribozymes tested. These results were derived from a trans-acting system. Here we tested this ribozyme activity in a cis-acting system. We show that the (-)s ArMV hairpin ribozyme has different target-site specificities between cis and trans cleavages. The wild type ribozyme has the highest self-cleaving activity among the ribozyme variants tested.",
author = "R. Tomita and Y. Morikawa and S. Hisamatsu and K. Suzuki-Fujita and T. Tanaka and S. Sonoki and Yo Kikuchi",
year = "1995",
language = "English",
pages = "117--118",
journal = "Nucleic acids symposium series",
issn = "0261-3166",
publisher = "Information Retrieval",
number = "34",

}

TY - JOUR

T1 - Cis and trans reactions of hairpin ribozymes derived from the negative strand of arabis mosaic virus satellite RNA.

AU - Tomita, R.

AU - Morikawa, Y.

AU - Hisamatsu, S.

AU - Suzuki-Fujita, K.

AU - Tanaka, T.

AU - Sonoki, S.

AU - Kikuchi, Yo

PY - 1995

Y1 - 1995

N2 - We have previously shown that the hairpin ribozyme-like structure of the negative strand of the satellite RNA of arabis mosaic virus [(- )sArMV] has indeed ribozyme activity. However, some mutagenesis analyses revealed surprisingly that the wild type ribozyme was less active than almost all the other mutant ribozymes tested. These results were derived from a trans-acting system. Here we tested this ribozyme activity in a cis-acting system. We show that the (-)s ArMV hairpin ribozyme has different target-site specificities between cis and trans cleavages. The wild type ribozyme has the highest self-cleaving activity among the ribozyme variants tested.

AB - We have previously shown that the hairpin ribozyme-like structure of the negative strand of the satellite RNA of arabis mosaic virus [(- )sArMV] has indeed ribozyme activity. However, some mutagenesis analyses revealed surprisingly that the wild type ribozyme was less active than almost all the other mutant ribozymes tested. These results were derived from a trans-acting system. Here we tested this ribozyme activity in a cis-acting system. We show that the (-)s ArMV hairpin ribozyme has different target-site specificities between cis and trans cleavages. The wild type ribozyme has the highest self-cleaving activity among the ribozyme variants tested.

UR - http://www.scopus.com/inward/record.url?scp=0029432390&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0029432390&partnerID=8YFLogxK

M3 - Article

SP - 117

EP - 118

JO - Nucleic acids symposium series

JF - Nucleic acids symposium series

SN - 0261-3166

IS - 34

ER -