Cis and trans reactions of hairpin ribozymes derived from the negative strand of arabis mosaic virus satellite RNA.

R. Tomita, Y. Morikawa, S. Hisamatsu, K. Suzuki-Fujita, T. Tanaka, S. Sonoki, Yo Kikuchi

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Abstract

We have previously shown that the hairpin ribozyme-like structure of the negative strand of the satellite RNA of arabis mosaic virus [(- )sArMV] has indeed ribozyme activity. However, some mutagenesis analyses revealed surprisingly that the wild type ribozyme was less active than almost all the other mutant ribozymes tested. These results were derived from a trans-acting system. Here we tested this ribozyme activity in a cis-acting system. We show that the (-)s ArMV hairpin ribozyme has different target-site specificities between cis and trans cleavages. The wild type ribozyme has the highest self-cleaving activity among the ribozyme variants tested.

Original languageEnglish
Pages (from-to)117-118
Number of pages2
JournalNucleic acids symposium series
Issue number34
Publication statusPublished - 1995
Externally publishedYes

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Tomita, R., Morikawa, Y., Hisamatsu, S., Suzuki-Fujita, K., Tanaka, T., Sonoki, S., & Kikuchi, Y. (1995). Cis and trans reactions of hairpin ribozymes derived from the negative strand of arabis mosaic virus satellite RNA. Nucleic acids symposium series, (34), 117-118.