Citric acid production from cellulose hydrolysate by a 2-deoxyglucose- resistant mutant strain of Aspergillus niger

T. Watanabe, A. Suzuki, H. Nakagawa, Kotaro Kirimura, S. Usami

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Citric acid production from cellulose hydrolysate was done by a semi- solid culture of Aspergillus niger Yang no. 2, a parental strain, and C192, which is a 2-deoxy-D-glucose-resistant mutant strain. Cellulose powder was hydrolyzed to mainly glucose and a low concentration of cellobiose by a commercial cellulase in acetate or citrate buffer. The hydrolysate prepared in acetate buffer was concentrated to contain 150 g/l of reducing sugars and used as a medium for fermentation. Yang no. 2 and C192 produced 77.9 g/l and 98.7 g/l of citric acid in 9 days from the concentrated hydrolysate, respectively. Acetate buffer delayed the fermentation. When the hydrolysate was prepared in citrate buffer and used as a medium, Yang no. 2 and C192 produced 92.2 g/l and 102.3 g/l of citric acid within 3 days from the concentrated hydrolysate containing 150 g/l of reducing sugars, respectively. It is concluded that citric acid production from cellulose hydrolysate with high conversion yield, maximally 68.2% based on the reducing sugars supplied, was achieved first by C192.

Original languageEnglish
Pages (from-to)271-274
Number of pages4
JournalBioresource Technology
Volume66
Issue number3
DOIs
Publication statusPublished - 1998 Dec
Externally publishedYes

Fingerprint

2-deoxyglucose
Aspergillus niger
Aspergillus
citric acid
Citric acid
Deoxyglucose
hydrolysates
Citric Acid
Cellulose
cellulose
Sugars
sugar
acetate
mutants
Buffers
buffers
reducing sugars
Fermentation
fermentation
Glucose

Keywords

  • Aspergillus niger
  • Cellulase
  • Cellulose hydrolysate
  • Citric acid
  • Deoxyglucose-resistant mutant
  • Semisolid culture

ASJC Scopus subject areas

  • Agronomy and Crop Science
  • Food Science
  • Process Chemistry and Technology
  • Applied Microbiology and Biotechnology

Cite this

Citric acid production from cellulose hydrolysate by a 2-deoxyglucose- resistant mutant strain of Aspergillus niger. / Watanabe, T.; Suzuki, A.; Nakagawa, H.; Kirimura, Kotaro; Usami, S.

In: Bioresource Technology, Vol. 66, No. 3, 12.1998, p. 271-274.

Research output: Contribution to journalArticle

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