Abstract
Post-transplant microbial diversity in the gastrointestinal tract is closely associated with clinical outcomes following allogeneic hematopoietic stem cell transplantation (allo-HSCT). However, little is known about the impact of the fecal microbiota before allo-HSCT. We analyzed fecal samples approximately 2 weeks before conditioning among 107 allo-HSCT recipients between 2013 and 2015. Microbial analysis was performed using 16S rRNA gene sequencing. Operational taxonomic unit-based microbial diversity was estimated by calculating the Shannon index. Patients were classified into three groups based on the diversity index: low (<2), intermediate (2, 3), and high (>3) diversity (18 (16.8%), 48 (44.9%), and 41 (38.3%) patients, respectively). There were no significant differences in the 20-month overall survival, cumulative incidence of relapse, and non-relapse mortality among three groups. The cumulative incidence of grade II to IV acute graft-versus-host disease (aGVHD) was similar among the three groups (low 55.6%; intermediate 35.4%; high 48.8%, p = 0.339, at day 100). Furthermore, we found no differences in the cumulative incidence of grade II to IV acute gastrointestinal GVHD among the three groups (low 38.9%; intermediate 21.3%; high 24.4%, p = 0.778, at day 100). Regarding the composition of microbiota before allo-HSCT, aGVHD patients showed a significantly higher abundance of phylum Firmicutes (p < 0.01) and a lower tendency for Bacteroidetes (p = 0.106) than non-aGVHD patients. Maintenance of Bacteroidetes throughout allo-HSCT may be a strategy to prevent aGVHD.
| Original language | English |
|---|---|
| Pages (from-to) | 1517-1523 |
| Number of pages | 7 |
| Journal | Annals of Hematology |
| Volume | 96 |
| Issue number | 9 |
| DOIs | |
| Publication status | Published - 2017 Sep 1 |
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Keywords
- Acute gastrointestinal graft-versus-host disease (GVHD)
- Allogeneic hematopoietic stem cell transplantation (allo-HSCT)
- Intestinal microbiota
- Microbial diversity
ASJC Scopus subject areas
- Hematology
Cite this
Clinical impact of pre-transplant gut microbial diversity on outcomes of allogeneic hematopoietic stem cell transplantation. / Doki, Noriko; Suyama, Masahiro; Sasajima, Satoshi; Ota, Junko; Igarashi, Aiko; Mimura, Iyo; Morita, Hidetoshi; Fujioka, Yuki; Sugiyama, Daisuke; Nishikawa, Hiroyoshi; Shimazu, Yutaka; Suda, Wataru; Takeshita, Kozue; Atarashi, Koji; Hattori, Masahira; Sato, Eiichi; Watakabe-Inamoto, Kyoko; Yoshioka, Kosuke; Najima, Yuho; Kobayashi, Takeshi; Kakihana, Kazuhiko; Takahashi, Naoto; Sakamaki, Hisashi; Honda, Kenya; Ohashi, Kazuteru.
In: Annals of Hematology, Vol. 96, No. 9, 01.09.2017, p. 1517-1523.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Clinical impact of pre-transplant gut microbial diversity on outcomes of allogeneic hematopoietic stem cell transplantation
AU - Doki, Noriko
AU - Suyama, Masahiro
AU - Sasajima, Satoshi
AU - Ota, Junko
AU - Igarashi, Aiko
AU - Mimura, Iyo
AU - Morita, Hidetoshi
AU - Fujioka, Yuki
AU - Sugiyama, Daisuke
AU - Nishikawa, Hiroyoshi
AU - Shimazu, Yutaka
AU - Suda, Wataru
AU - Takeshita, Kozue
AU - Atarashi, Koji
AU - Hattori, Masahira
AU - Sato, Eiichi
AU - Watakabe-Inamoto, Kyoko
AU - Yoshioka, Kosuke
AU - Najima, Yuho
AU - Kobayashi, Takeshi
AU - Kakihana, Kazuhiko
AU - Takahashi, Naoto
AU - Sakamaki, Hisashi
AU - Honda, Kenya
AU - Ohashi, Kazuteru
PY - 2017/9/1
Y1 - 2017/9/1
N2 - Post-transplant microbial diversity in the gastrointestinal tract is closely associated with clinical outcomes following allogeneic hematopoietic stem cell transplantation (allo-HSCT). However, little is known about the impact of the fecal microbiota before allo-HSCT. We analyzed fecal samples approximately 2 weeks before conditioning among 107 allo-HSCT recipients between 2013 and 2015. Microbial analysis was performed using 16S rRNA gene sequencing. Operational taxonomic unit-based microbial diversity was estimated by calculating the Shannon index. Patients were classified into three groups based on the diversity index: low (<2), intermediate (2, 3), and high (>3) diversity (18 (16.8%), 48 (44.9%), and 41 (38.3%) patients, respectively). There were no significant differences in the 20-month overall survival, cumulative incidence of relapse, and non-relapse mortality among three groups. The cumulative incidence of grade II to IV acute graft-versus-host disease (aGVHD) was similar among the three groups (low 55.6%; intermediate 35.4%; high 48.8%, p = 0.339, at day 100). Furthermore, we found no differences in the cumulative incidence of grade II to IV acute gastrointestinal GVHD among the three groups (low 38.9%; intermediate 21.3%; high 24.4%, p = 0.778, at day 100). Regarding the composition of microbiota before allo-HSCT, aGVHD patients showed a significantly higher abundance of phylum Firmicutes (p < 0.01) and a lower tendency for Bacteroidetes (p = 0.106) than non-aGVHD patients. Maintenance of Bacteroidetes throughout allo-HSCT may be a strategy to prevent aGVHD.
AB - Post-transplant microbial diversity in the gastrointestinal tract is closely associated with clinical outcomes following allogeneic hematopoietic stem cell transplantation (allo-HSCT). However, little is known about the impact of the fecal microbiota before allo-HSCT. We analyzed fecal samples approximately 2 weeks before conditioning among 107 allo-HSCT recipients between 2013 and 2015. Microbial analysis was performed using 16S rRNA gene sequencing. Operational taxonomic unit-based microbial diversity was estimated by calculating the Shannon index. Patients were classified into three groups based on the diversity index: low (<2), intermediate (2, 3), and high (>3) diversity (18 (16.8%), 48 (44.9%), and 41 (38.3%) patients, respectively). There were no significant differences in the 20-month overall survival, cumulative incidence of relapse, and non-relapse mortality among three groups. The cumulative incidence of grade II to IV acute graft-versus-host disease (aGVHD) was similar among the three groups (low 55.6%; intermediate 35.4%; high 48.8%, p = 0.339, at day 100). Furthermore, we found no differences in the cumulative incidence of grade II to IV acute gastrointestinal GVHD among the three groups (low 38.9%; intermediate 21.3%; high 24.4%, p = 0.778, at day 100). Regarding the composition of microbiota before allo-HSCT, aGVHD patients showed a significantly higher abundance of phylum Firmicutes (p < 0.01) and a lower tendency for Bacteroidetes (p = 0.106) than non-aGVHD patients. Maintenance of Bacteroidetes throughout allo-HSCT may be a strategy to prevent aGVHD.
KW - Acute gastrointestinal graft-versus-host disease (GVHD)
KW - Allogeneic hematopoietic stem cell transplantation (allo-HSCT)
KW - Intestinal microbiota
KW - Microbial diversity
UR - http://www.scopus.com/inward/record.url?scp=85025454802&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85025454802&partnerID=8YFLogxK
U2 - 10.1007/s00277-017-3069-8
DO - 10.1007/s00277-017-3069-8
M3 - Article
VL - 96
SP - 1517
EP - 1523
JO - Annals of Hematology
JF - Annals of Hematology
SN - 0939-5555
IS - 9
ER -